RU2344171C1 - Method of manufacturing proteolytic enzyme preparation from fish visceral organs - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: method implies raw material washing, crushing, preserving, autolysating, centrifuging to separate liquid enzyme preparation, packing and storing. Raw material is rinsed with the anolyte of electrochemically activated water after washing. Sodium chloride in the amount of 3-4% to the raw material weight is added when preserving. Autolysis is carried out using electrochemically activated water catholyte in the amount of 30% to the raw material weight for 2 hours at longest under the temperature of 40°C.

EFFECT: reducing autolysis time and decreasing amount of the preserving agent added.

1 ex

Description

The invention relates to the fishing industry and is intended for the production of a proteolytic enzyme preparation used for the production of food products from low-ripening fish.

A known method of obtaining the enzyme preparation "Ocean" from the insides of mackerel, horse mackerel and sardinella. The method is carried out due to the autolysis of the insides of these fish, while the insides are used entirely, but provided that the mass of fats and gonads does not exceed 20% of the mass of raw materials. Raw materials are preserved by adding 10% table salt to its mass (see Golenkova V.V., Nekrasova G.T. Technology of the enzyme preparation "Ocean" and its modifications // Sat. Scientific Research Papers AtlantNIRO. Progressive production technology preserves, salted and smoked fish products, 1988, p. 67-90).

The disadvantages of this method are the presence of fats and gonads in the feed, which increase the duration of hydrolysis of the feed and reduce the enzymatic activity of the resulting enzyme preparation, as well as preservation of sodium chloride in the amount of 10% by weight of the feed, which reduces the activity of enzymes having a protein nature.

A known method of producing a proteolytic enzyme preparation from internal organs of fish (see RF patent No. 2288951, 2006), which includes grinding the raw material, its autolysis in the presence of water at a water module of 1: 0.5 at a pH of 6.2 ± 0.2 V within 1-5 hours, separation of the liquid autolysate by centrifugation, its purification with isopropyl alcohol at a hydromodule 3: 1 for 30-40 minutes, drying at a temperature of 150-180 ° C for 5-8 s.

The disadvantages of this method are:

- raw materials are not preserved, which can lead to the development of microflora and spoilage during the process of autolysis;

- Isopropyl alcohol, which is used to clean the enzyme preparation, is a toxic substance and traces of it must be removed.

Closest to the proposed method is a method for producing an enzyme preparation of proteolytic action from the entrails of fresh and frozen fish (see RF patent No. 13399917, 1994), including grinding the entrails without reproductive products of fresh or frozen fish (herring, ivasi, Pacific herring, mackerel, salmon, capelin, rasp and other ripening fish), preserving them with sodium chloride in an amount of 6-8% by weight of raw materials, keeping at a natural physiological pH value at a temperature of 0-25 ° C for 2-3 hours, centrifuges ery for separation of the liquid fraction - the enzyme preparation, packaging of the enzyme preparation and its storage at a temperature of from 5 to -5 ° C.

The disadvantages of this method are:

- preservation of the viscera is carried out with sodium chloride in an amount of 6-8% by weight of the raw material, which leads to a decrease in the activity of the enzyme system;

- the hydrolysis process is carried out without the participation of water, only due to moisture contained in the raw material, which reduces the yield of the enzyme preparation.

The technical task of the invention is a method for producing an enzyme preparation of proteolytic action from the internal organs of fish, involving the use of anolyte ECA-water for rinsing raw materials and catholyte for the process of autolysis.

The technical result of the proposed method is to improve the method: reducing the amount of preservative (sodium chloride) introduced, reagentless creation of a pH of the medium, reducing the duration of the autolysis process, increasing the sanitary culture of production.

It is achieved by the fact that after washing, the internal organs of fish without genital products are rinsed with anolyte of electrochemically activated (ECA) water, and the autolysis process is carried out using catholyte in an amount of 30% by weight of the raw material for no more than 2 hours at a temperature of 40 ° C with addition of 3 -4% to the mass of raw materials of sodium chloride for canning.

The method is as follows.

Example 1

The internal organs of fish without sexual products are washed, rinsed with an anolyte of ECA water to reduce bacterial contamination, crushed, preserved by adding sodium chloride in an amount of 3-4% to the mass of raw materials, such a small amount of which is due to the presence of catholyte ECA water with a bacteriostatic effect which is used in autolysis. The insides are autolysed for no more than 2 hours at a temperature of 40 ° C in the presence of ECA water catholyte, which allows creating an pH of 7.0-8.0, which is optimal for activating neutral-alkaline proteases of internal organs, in addition, the catholyte has bacteriostatic action, which allows the process to be carried out without preservatives, by centrifugation for 20-30 minutes at a rotational speed of the centrifuge of 3000-3500 rpm, a liquid autolysate containing proteolytic enzymes is separated. The resulting enzyme preparation is packaged and stored at a temperature of from 0 to -5 ° C.

A positive effect of the invention is a decrease in the amount of added preservative (sodium chloride), reagent-free creation of a pH of the medium, a decrease in the duration of the autolysis process, and an increase in the sanitary culture of production.

Information sources

1. Golenkova V.V., Nekrasova G.T. Technology of the enzyme preparation "Ocean" and its modifications // Sat. scientific tr AtlantNIRO. Progressive technology for the production of preserves, salted and smoked fish products, 1988, p. 67-90.

2. RF patent No. 2288951, 2006

3. RF patent No. 1339917, 1994 (prototype).

Claims (1)

A method of obtaining a proteolytic enzyme preparation from the internal organs of fish without reproductive products, comprising washing the raw material, crushing, preserving, autolysis, centrifuging to separate the liquid enzyme preparation, its packaging and storage, characterized in that after washing the raw material is rinsed with anolyte of electrochemically activated water , during preservation, sodium chloride is added in an amount of 3-4% to the mass of raw materials, and autolysis is carried out using catholyte electrochemically activated water in an amount of 30% by weight of the raw material for no more than 2 hours at a temperature of 40 ° C.