RU2324715C1 - Method of concentrating anthocyan dye out of water solutions - Google Patents

Abstract

FIELD: technological processes.

SUBSTANCE: technical task of invention is reduction of concentrate preparation time. Task solution is achieved since in order to concentrate anthocyan dye out of water solutions, organic reagent extraction is applied, and organic reagent is water soluble polymer poly-N-vinylpyrrolidone, which is added in the amount of 0.1-0.5% from the initial solution mass. At that extraction is carried out in the presence of salting-out agent - ammonium sulfate in the amount of 40-43% from the initial solution mass.

EFFECT: increases extraction of anthocyan dye out of water solution during single extraction and reduces time of concentrate preparation to 3-5 minutes.

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8916969

Description

The invention relates to biotechnology for the production of food colors and can be recommended for use in the food, perfume, cosmetics and pharmaceutical industries.

The closest in technical essence and the achieved effect is a method of concentrating food dye, based on three times the extraction of the dye from plant materials with an organic reagent (96% ethanol) at 40-60 ° C, keeping the solution for 8-12 hours and subsequent distillation of ethanol [Patent 2220172 RU, MKI 7 С09В 61/00. A method of obtaining anthocyanin dye from flower raw materials. Odin A.P., Khayrutdinova A.D., Bolotov V.M. - VGTA. No. 2002119475/13; Declared July 17, 2002, publ. 12/27. 2003, bull. No. 36, part II].

The disadvantage of this method is the duration of the concentrate.

An object of the invention is to reduce the time to obtain an anthocyanin dye concentrate from aqueous solutions.

The stated technical problem is achieved by the fact that in the method of concentrating anthocyanin dye from aqueous solutions, which involves the extraction of anthocyanin dye from aqueous solutions with an organic reagent, it is new that a water-soluble polymer poly-N-vinylpyrrolidone is added in an amount of 0.1 -0.5% by weight of the initial solution, while extraction is carried out in the presence of an ammonium sulfate salting out agent in an amount of 40-43% by weight of the initial solution.

The technical result consists in the intensification of the process of obtaining concentrate.

The proposed method for concentrating anthocyanin dye from aqueous solutions is as follows.

13.3-15.1 g of crystalline ammonium sulfate (40-43 wt.%) Is added to 20 cm ^{3 of an} aqueous solution of anthocyanin dye, the optical density of the aqueous dye solution (A) is measured, 0.02-0.1 g is introduced into the solution poly-N-vinylpyrrolidone (0.1-0.5 wt.%) and extracted. In the presence of ammonium sulfate, poly-N-vinylpyrrolidone (PVP) forms an independent phase, which extracts the dye. Interfacial equilibrium is achieved within 3-5 minutes. The concentrate is separated by filtration, and the optical density of the equilibrium aqueous solution (A _p ) is measured. The content of anthocyanin dye in the equilibrium aqueous phase is determined spectrophotometrically by its own light absorption (KFK-2MP, λ = 490 nm). Calculate the degree of extraction (R,%) of the anthocyanin dye by the equation:

R = (A-A _p ) 100% / A.

98-99% of anthocyanin dye passes into the organic phase. The concentration method is illustrated by the following examples.

Example 1. To 20 cm ^{3 of an} aqueous solution of anthocyanin dye add 13.3 g of crystalline ammonium sulfate (get a solution with a mass fraction of 40%), measure the optical density of the aqueous solution (A), enter 0.04 g of poly-N-vinylpyrrolidone ( PVP content in an aqueous solution of 0.2 wt.%) and extracted for 4 minutes The concentrate is separated by filtration, the optical density of the equilibrium aqueous solution (A _p ) is measured, the degree of extraction (R,%) of the anthocyanin dye is calculated by the equation:

R = (A-A _p ) 100% / A.

98% of anthocyanin dye passes into the organic phase.

Example 2. To 20 cm ^{3 of an} aqueous solution of anthocyanin dye add 15.1 g of crystalline ammonium sulfate (get a solution with a mass fraction of 43%), enter 0.04 g of PVP and extract for 4 minutes Further analysis is performed analogously to example 1.

98% of anthocyanin dye passes into the organic phase.

Extraction with a more diluted solution of PVP (<0.1%) and (NH ₄ ) ₂ SO ₄ (<40%) does not completely remove the dye, and an increase in the concentration of the PVP solution (> 0.5%) is not economically feasible.

Comparative characteristics of the known and proposed methods for the extraction and concentration of anthocyanin dye from aqueous solutions are given in the table.

Table Indicators Known method The proposed method The degree of extraction of anthocyanin dye,% - 98-99 Concentrate production time 12 h 3-5 min The number of operations required to obtain the concentrate 5 2

The proposed method of concentration allows to intensify the process of obtaining a concentrate of anthocyanin dye due to a single extraction with poly-N-vinylpyrrolidone.

Claims (1)

A method for concentrating anthocyanin dye from aqueous solutions, involving the extraction of anthocyanin dye from aqueous solutions with an organic reagent, is new in that a water-soluble poly-N-vinylpyrrolidone polymer is added as an organic reagent, added in an amount of 0.1-0.5% by weight of the starting material solution, while the extraction is carried out in the presence of a salting out agent of ammonium sulfate in an amount of 40-43% by weight of the initial solution.