RU2297762C1 - Method for increasing of poultry pullorosis resistance - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: method involves pre-incubation processing of incubation eggs using helium-neon laser light having wavelength of 632.8 nm, optical flux intensity of 50 mW/cm²·s; combining pre-incubation processing of eggs by helium-neon laser light with treatment of incubation eggs before incubation on 6, 12 and 18 incubation days by gas-discharge lamp light having wavelength of 630-650 nm, with mean dose of 23.1 erg on egg surface, mercury-quartz lamp light having wavelength of 400/185 nm, with mean dose of 20 mer/hour on egg surface, and two bactericidal lamps having wavelength of 254/800 nm and nominal power of 15 W on egg surface with exposure time of 3 min. Method allows resistance of poultry to pullorosis-5, pullorum pathogen to be increased.

EFFECT: improved specific viability of poultry and increased disease resistance.

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Description

The invention relates to poultry farming and can be, first of all, applied to increase the safety and productivity of poultry.

There is a method of increasing the viability of chickens by pre-incubation processing of eggs with radiation from four OKG-12 helium-neon lasers with a wavelength of 632.8 nm, an output power of 15 mW / cm ² , in which growth, development, and indicators of natural resistance of the offspring are stimulated / Petrov EB The use of helium-neon laser rays to stimulate the embryogenesis of chickens and increase the viability of chickens - abstract of diss. Cand. Agricultural Sciences M .: MVA - 1982. - 20 /.

There is a method for the prevention of bird pullorosis by pre-incubation treatment of eggs with radiation of a LGN-104 helium-neon laser, a wavelength of 632.8 nm, a power density on the surface of eggs of 50 mW / cm ² · s (Mamukaev M.N. Patent No. 2193843, 2002 prototype).

The disadvantages of the known methods is that they do not reveal the specific resistance of the bird to pullorosis during the complex pre-incubation treatment of eggs with the radiation of a LGN-104 helium-neon laser, a DnESG-500 gas-discharge lamp, ultraviolet lamps DRT-400 and BUV-15, the activity of poultry blood serum is not determined obtained from irradiated eggs relative to the causative agent of pulmonosis Salmonella pullorum, microbiological and serological studies have not been carried out with a comprehensive pre-incubation and incubation treatment of bird embryos.

The purpose of the invention is to increase the specific viability of the bird to the effects of S. pullorum pullorosis pathogen under conditions of dysfunctional poultry farming. This goal is achieved by the fact that large embryos before incubation, embryos on the 6th, 12th and 18th days of development are heated with the light of a DnESG-500 gas-discharge lamp with a wavelength of 630-650 nm, an average dose on the egg surface of 23.1 erg, and then with a helium-neon laser LGN-104, a wavelength of 632.8 nm, a power density on the egg surface of 50 mW / cm ² · s, a DRT-400 mercury-quartz lamp, a wavelength of 400/185 nm, an average dose on the egg surface of 20 mer / h and two BUV-15 bactericidal lamps with a wavelength of 254/800 nm, rated power of 15 W in expositions of 3 minutes.

The treatment of incubation eggs with radiant energy was carried out in an experimental setup for light-laser activation / Mamukaev M.N. Auth. testimonial. No. 1748768, 1989 /.

Studies of the influence of light energy on the blood serum activity of experimental broiler chickens showed that the light-laser treatment of eggs and developing embryos in optimal doses significantly contributes to an increase in the bactericidal and bacteriostatic activity of blood serum relative to the pathogen Salmonella pullorum pullorosis (Table 1).

At 0.75 hours the reaction of the blood serum of daily broilers and S.pullorum, relative to the results of the control group,% lysis in the 2nd group was significantly higher by 2.09%, in 3 - by 0.50, in 4 - by 1.60 and in group 5 - by 2.75% lysis, respectively, after 8 hours of reaction - by 2.57; 0.78; 2.52; and 3.45%, 16 hours - 2.27; 0.93; 2.10 and 3.13% and after 24 hours of reaction - by 2.30; 1.20; 0.80 and 3.09% lysis. Differences in control and 4 groups of birds at 24 hours of the reaction were unreliable (P> 0.05).

Thus, the bactericidal activity of the blood serum of experimental chickens relative to S.pullorum shows that a stable increase in this indicator is observed in the groups of laser, red and ultraviolet rays in the complex, as well as when using laser beams. In the ultraviolet group, after 16 hours of incubation of the ingredients, a rapid decline in the bactericidal activity of blood serum is observed and reaches the control level.

As a result, a natural increase in the bactericidal activity of blood serum is observed up to 8 hours of incubation and a decrease in results after 8 hours, reaching minimal differences with the control. An exception is the group of complex irradiation of embryos, where the results are higher and at the end of the experiments.

In monthly broilers, the activity of blood serum in all experimental groups was higher than in the control. The exception is group 4, where the difference with the control group at 24 h of studies was minimal and amounted to 0.55% lysis at P> 0.05.

Table 1 Bactericidal and bacteriostatic activity of broiler blood serum relative to S.pullorum, (X ± m _x ),% lysis, n = 10 Group Bird age, days one thirty Reaction time, h 0.75 8 16 24 0.75 8 16 24 1 control 4.73 ± 0.18 6.16 ± 90.24 5.11 ± 0.22 1.09 ± 0.18 4.87 ± 0.24 6.18 ± 0.13 4.13 ± 0.30 2.12 ± 0.19 2 experienced 0.82 ± 0.19 8.73 ± 0.21 7.38 ± 0.21 3.39 ± 0.15 6.71 ± 0.19 8.94 ± 0.21 6.55 ± 0.22 4.46 ± 3.21 3 experienced 5.23 ± 0.14 6.94 ± 0.19 6.04 ± 0.21 2.29 ± 0.20 5.49 ± 0.20 7.73 ± 0.21 5.18 ± 0.20 3.11 ± 0.24 4 experienced 6.33 ± 0.20 8.68 ± 0.23 7.21 ± 0.14 1.89 ± 0.18 6.85 ± 0.21 8.91 ± 0.24 6.11 ± 0.24 2.67 ± 0.22 5 experienced 7.48 ± 0.15 9.61 ± 0.23 8.24 ± 0.20 4.00 ± 0.27 7.41 ± 0.23 9.77 ± 0.20 7.09 ± 0.31 5.34 ± 0.20

Summarizing the results of the studies, it should be noted that the determination of the bactericidal and bacteriostatic activity of blood serum relative to S.pullorum can be recommended as a test to determine the resistance of birds with typhoid pallorosis. Our studies indicate a higher degree of resistance of the bird organism obtained from eggs and embryos after light treatment before laying for incubation and during embryonic development. The highest results were recorded in the group of young animals obtained from eggs after complex treatment with laser, red and ultraviolet light. When comparing the use of laser light and ultraviolet radiation up to 16 hours of incubation of the ingredients, no significantly distinguishable results were found, however, after 16 hours in the ultraviolet group, the serum activity decreases more sharply and reaches the control values in day old chickens. Regarding the application group of monochromatic red light, laser light is more active in stimulating the bactericidal and bacteriostatic properties of blood serum.

Studies of the viability, microbiological and serological parameters of experimental broiler chickens obtained by light-laser activation of embryogenesis (Table 2) give reason to draw the following conclusions:

- the safety of broilers obtained from eggs and embryos after complex treatment with laser, red and ultraviolet light amounted to 98.5% by 30 days of cultivation, which is higher than the results of the control group by 4.0%, the use of laser light - 0.5%, red and ultraviolet light - by 1.5%;

- in the control group of 11 chickens the causative agent of pullorosis S. pullorum was isolated in 5, respectively, in the group using laser light 4 and 3, red light 6 and 3, ultraviolet light 6 and 2, complex exposure 3 and 1;

table 2 Microbiological and serological studies of monthly broilers with light laser activation, n = 200 Group Sources Processing object Saved Goal It’s fallen, a goal. Highlighted S.pullorum, samples Reacted positively for PA samples Had contact with S.pullorum, goal. 1 - control - - 189 eleven 5 25 thirty 2 - experienced LGN-104 Embryo 196 four 3 25 29th 3 - experienced DNESG-500 - 194 6 3 27 thirty 4 - experienced DRT-400 - 194 6 2 25 27 5 - experienced LGN-104 - 197 3 one 23 24 DNESG-500 - DRT-400 BUV-15

- positively responded by RA to the pullorose antigen in the control group of 25 samples, in the group of application of laser light 26, red 27, ultraviolet 25 and with a combined exposure to 23 samples;

- according to the results of microbiological and serological studies of broilers up to one month old, 30 chickens were in contact with S. pullorum pullorosis pathogen in the control group, 5 of them fell, mortality was 16.7%, respectively, when exposed to laser light - 29; 3 and 10.3%, red light - 30; 3 and 10.0%, ultraviolet light - 27; 2 and 7.4% and complex exposure - 24; 1 and 4.2%;

Thus, indicators of overall viability, poultry survival under conditions of poor pallorosis economy, bactericidal and bacteriostatic activity of blood serum relative to the causative agent of S. pullorum pullorosis, serological and microbiological studies show that the use of the proposed method for pre-incubation and incubation complex treatment of chicken embryos with LHN laser radiation 104, lamps DNESG-500, DRT-400 and BUV-15 in expositions of 3 minutes each, increase the bird's resistance to the effects of the pathogen pu llorose - typhoid bird S.pullorum

Claims (1)

A method of increasing bird viability for pullorosis, including pre-incubation treatment of hatching eggs with helium-neon laser light with a wavelength of 632.8 nm, an optical flux power on the egg surface of 50 mW / (cm ² · s), characterized in that the pre-incubation treatment of eggs with helium light -neon laser was combined with the treatment of hatching eggs before incubation on the 6th, 12th and 18th days of incubation with light of a DNESG-500 gas-discharge lamp with a wavelength of 630-650 nm, an average dose on the egg surface of 23.1 erg, and a DRT-400 mercury-quartz lamp with wavelength 400/185 nm, the average dose on the surface of the eggs is 20 mer / h and two bactericidal lamps BUV-15 with a wavelength of 254/800 nm, the rated power on the surface of the eggs is 15 W in exposures of 3 min.