RU2276785C1 - Method for studying biological activity of water - Google Patents

Abstract

FIELD: ecology.

SUBSTANCE: the present innovation deals with testing biological activity of water, preliminary treatment of water, division into control and tested portions, ionization of tested portion with silver ions, detection of the quantity of sprouted wheat grains per time unit pre-impregnated in both mentioned portions and calculation of relative alteration for the value of biological activity of water according to the following ratio:

where d - relative alteration for the value of biological activity of water, %; N_tested - the quantity of sprouted grains per time unit in tested portion of water, pcs.; N_control - the quantity of sprouted grains per time unit in control portion of water, pcs. Moreover, relative alteration for the value of biological activity of water being above 0 means increased biological activity of water, relative alteration for the value of biological activity of water being below 0 means its decrease, and equation of the mentioned relative alteration to 0 means intact nature of biological activity of water being different by the fact that pre-treatment of water should be carried out due to precipitation for 23-24 h at 23-26 C.

EFFECT: higher efficiency of investigation.

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Description

The invention relates to ecology and can be used to study the biological activity of water and aqueous solutions under informational influences.

There is a method of researching the biological activity of water (BAS) by dividing water into the control and test parts, providing informational impact on the test part and BAS research on determining the number of sprouted wheat grains per unit time in the control and test parts (Goskov P.I. Information transfer by water . // Bioinformatics and bioenergy information technologies (BEIT-2000): Reports of the 3rd International Congress, T.1. - Barnaul: AltSTU Publishing House, 2000. S.11-14).

The disadvantage of the described method is the low accuracy due to the increase in the random component of the error and the lack of repeatability of the research results due to the large spread of these results, due to the instability of the structure of the water that has not undergone preliminary processing.

Closest to the proposed invention in terms of technical nature and the achieved result (prototype) is a method for studying the biological activity of water by pretreating the water, separating it into the control and test parts, providing informational impact on the test part and BAS studies to determine the number of sprouted wheat grains per unit time in the control and investigated parts. In this case, the pretreatment is carried out by heating water to 100 ° C, and the information impact is exerted by ionization of water by silver ions (Bobrov A.V. Study of factors determining the biological activity of water. / Field information interactions // Proceedings. - Orel: Orel State Technical University, 2003, p. 378-430).

The disadvantage of the described method is the low accuracy due to the increase in the random component of the error and the presence of a large variation in the results of the study, due to the restoration of the water structure to its original state after preliminary treatment by heating it to 100 ° C.

The proposed invention solves the problem of increasing the accuracy of the method of research of biologically active substances.

To achieve this technical result in the proposed method for studying the biological activity of water by pretreating water, separating it into the control and test parts, ionizing the test part with silver ions, determining the number of sprouted wheat grains per unit time, soaked in both of these parts, and calculating the relative change indicator of biological activity of water from the ratio

where d is the relative change in the index of biological activity of water,%;

N _studied - the number of sprouted grains per unit time in the studied part of the water, pcs .;

N _control - the number of sprouted grains per unit time in the control part of the water, pcs.,

the relative change in the indicator of biological activity of water greater than zero means an increase in biological activity, the relative change in the indicator of biological activity of water less than zero means its decrease, and the equality of the indicated relative change to zero means the invariability of the biological activity of water, pretreatment of water is carried out by settling for 23-24 hours at a temperature of 23-26 ° C.

Improving the accuracy of the method for studying biologically active substances is provided by reducing the random component of the error due to an increase in the sensitivity of the method due to the fact that after the preliminary treatment of the water with sedimentation for 23-24 hours at 23-26 ° C, the water structure remains stable.

The pretreatment of water by settling for 23-24 hours is optimal, since settling water for less than 23 hours leads to an increase in the random component of the measurement error due to the fact that the water structure has not reached stability, and more than 24 hours is impractical, as it leads to increase the duration of the study. The dependence of the random measurement error in the study of biologically active substances on the type of pretreatment of water is given in table 1.

Water sedimentation at a temperature of 23-26 ° C is optimal due to the creation of the best temperature conditions for the germination of wheat grains, since at a temperature of less than 23 ° C the biological activity of water decreases, and at a temperature of more than 26 ° C there is a rapid germination of wheat grains, which leads to decrease in sensitivity.

A method for studying the biological activity of water is as follows.

Pre-treatment of water is carried out by settling for 23-24 hours at a temperature of 23-26 ° C.

After that, the water is divided into control and test parts. The control part preserves the initial water structure.

They have an effect on the studied part by ionization of water with silver ions.

To determine the number of sprouted wheat grains per unit time, a certain number of batches containing, for example, 100 wheat grains, are counted in the control and test sections. Each batch of grains is placed in a separate gauze napkin. At least five batches are laid out in a plastic container for soaking in the control and test portions of water. Thus, five batches in one plastic container make up one experimental point. As an indicator of the biological activity of water, grains of higher grades of durum wheat are used. The prepared batches are poured with the control and test portions of water so that the gauze napkins with grain are completely covered with water. After that, water is removed from plastic containers. Each experimental point is labeled and placed in a separate plastic bag. The laying time is considered the moment of soaking wheat grains. Control the germination of grains after 24, 48 and 72 hours from the moment of soaking. In each batch of 100 grains, the number of sprouted grains in the control and studied parts is determined. Their mean value and standard deviation for each experimental point are calculated with a probability of at least 0.95. For the study of biologically active substances, the relative change in the biologically active index is calculated (A. Bobrov, Investigation of the factors determining the biological activity of water. / Field informational interactions // Proceedings. - Orel: Orel State Technical University, 2003. p.378-430) by the formula:

where d is the relative change in the index of biologically active substances,%;

N _studied - the number of sprouted grains per unit time in the studied part of the water, pcs .;

N _control - the number of sprouted grains per unit time in the control part of the water, pcs.

If the relative change in the biologically active index is greater than zero, then the biologically active substance increases with the informational impact. If the relative change in the biologically active index is less than zero, then the biologically active substance decreases by the informational impact. If the relative change in the index of biologically active substances is zero, then the biological activity as a result of information exposure does not change.

Thus, the use of the proposed method improves the accuracy of the measurement of biologically active substances.

Table 1
The dependence of the random measurement error in the study of biologically active substances on the type of pre-treatment of water Water pretreatment Water heating to 100 ° C (according to the prototype) Water sedimentation according to the claimed method 10 h 24 h 48 h σ,% twenty 10 5 5

Claims (1)

A method for studying the biological activity of water by pretreating water, separating the control and test parts, ionizing the test part with silver ions, determining the number of sprouted wheat grains per unit time previously soaked in both of these parts, and calculating the relative change in the index of biological activity of water from the ratio

where d is the relative change in the index of biological activity of water,%; N _studied - the number of sprouted grains per unit time in the studied part of the water, pcs .; N _control - the number of sprouted grains per unit time in the control part of the water, pcs., wherein the relative change in the indicator of biological activity of water greater than zero means an increase in biological activity, the relative change in the indicator of biological activity of water less than zero means its decrease, and the equality of the indicated relative change to zero means the invariability of the biological activity of water, characterized in that the pretreatment of water is carried out by settling for 23-24 hours at a temperature of 23-26 ° C.