RU2275201C2 - Haemostatic agent - Google Patents

Abstract

FIELD: pharmaceutical industry, in particular haemostatic agent.

SUBSTANCE: claimed agent represents aqueous solution, containing 2-150 g of zinc polyacrylate, 10-100 g sodium alginate and 1-30 of acetic acid in 1 l of distilled water. Agent of present invention being in contact with blood and tissues provides film formation 30 s.

EFFECT: haemostatic agent of improves quality.

Description

The invention relates to medicine and can be used in surgical practice.

Fibrin glue (V. Tilsver, N. Heimburgen, U. Becker et. Al Gerinnungs diagnostik fur Diagnose und Therapie, Behringwerke AG 1986, Marburg / Fankfurt, is known and widely used in the USA and Europe, has the ability to stop bleeding and glue tissues). / M). It is a system consisting of two vials, one of them includes thrombin (400-600 ME) and calcium chloride (40 mmol / l), the second - fibrinogen (65-115 mg), factor X111 (40-80 units ), aproginin (1000 KIE). If bleeding occurs, the surface is drained and solutions from both bottles are applied to the wound. As a result, thrombin and calcium facilitate the conversion of fibrinogen to fibrin, factor XIII (activated by calcium) strengthens the fibrin filaments, and aproginin protects the resulting fibrin clot from fibrinolysis.

Fibrin glue has found widespread use not only for stopping bleeding, but also for gluing tissues (along with suturing) for skin grafting, tearing of the liver, spleen, pancreas, bonding of connective tissue during cataract surgery, timpatoplasty, closing defects after tumor resection bonding osteochondral fragments, etc.

However, fibrin glue has a number of disadvantages, it consists of expensive proteins, the shelf life of which is only 2 years. It stops bleeding and causes tissue adhesion only after preliminary drainage of the surface. It is a favorable factor for the growth of microorganisms due to the fact that the glue contains proteins. It can be the cause of the development of allergic reactions. The time to stop bleeding with fibrin glue is 150 seconds. However, it does not provide strong gluing of damaged tissues to each other; blood leakage significantly interferes with gluing.

In connection with the foregoing, we proposed a new original hemostatic adhesive, which is an aqueous solution containing 2-15% zinc polyacrylate, 1-10% sodium alginate and 0.1-3% acetic acid. For its preparation, 10-100 g of sodium alginag, 20-150 g of zinc polyacrylate and 1-30 ml of acetic acid are dissolved in 1 liter of distilled species.

The proposed hemostatic glue stops bleeding, forming a film in contact with blood and tissues in 30 seconds.

Glue is applied to the wound from one syringe, uniformly filling the wound surface, without draining the latter.

Unlike expensive fibrin glue, it consists of industrially produced ingredients. It can be stored at room temperature for 5 years. Thus, it is convenient to use it in the field, in the practice of the Ministry of Emergencies. It passes well through the probes and therefore can be used in endoscopic practice.

Example 1

Experiments to study the effect of hemostatic glue on the function of hemostasis were carried out in laboratory conditions on chinchilla rabbits of both sexes weighing 2000-4000 g. Experiments to evaluate the hemostatic effect of various drugs were performed under thiopental anesthesia (2-4 ml of a 0.1% solution intravenously, and then up to 6-10 ml of a 0.1% solution intraperitoneally). A laparotomy was performed using a longitudinal section along the white line of the abdomen. Intestine was taken out into the wound, limiting it with napkins moistened with warm saline and the front surface of the liver. With the help of a special limiting device, a superficial razor of the liver was applied with a sharp razor about 1.5 cm square and about 0.1 cm deep. The development of capillary-parenchymal bleeding was stopped by uniformly applying 1 ml of hemostatic glue containing 2% zinc polyacrylate 1% sodium alginag and 0.1% acetic acid, after 32 seconds a dense film of glue is formed, which is not washed off by the blood stream. It adheres well to the wound surface. When removing the film, bleeding does not resume. As a control, we stopped the bleeding by tightly pressing a tampon from a gauze napkin to the wound surface. In this case, the bleeding stopped in 300 seconds.

Example 2

Experiments to study the effect of hemostatic glue on the function of hemostasis were carried out in laboratory conditions on chinchilla rabbits of both sexes weighing 2000-4000 g. Experiments to evaluate the hemostatic effect of various drugs were performed under thiopental anesthesia (2-4 ml of a 0.1% solution intravenously, and then up to 6-10 ml of a 0.1% solution intraperitoneally). Laparotomy was performed using a longitudinal section along the white line of the abdomen. Intestine was taken out into the wound, limiting it with napkins moistened with warm saline solution, an incision was made on two segments of the colon and anastomosis was applied side to side. 1 ml of hemosomatic glue containing 15% zinc polyacrylate, 10% sodium alginate and 3% acetic acid was evenly applied to the seam. After 20 seconds, a dense film was formed, which was not washed off by blood flow. It adheres well to the wound surface. When removing the film, bleeding does not resume. As a control, we stopped the bleeding by tightly pressing a tampon from a gauze napkin to the wound surface. Bleeding stopped in 240 seconds.

Example 3

The experiments on the effect of hemostatic glue on the function of hemostasis were carried out under laboratory conditions on chinchilla rabbits of both sexes weighing 2000-4000 g. The experiments on assessing the hemostatic effect of various drugs were performed under thiopental anesthesia (2-4 ml of a 0.1% solution intravenously, and then up to 6-10 ml of a 0.1% solution intraperitoneally). Laparotomy was performed using a longitudinal section along the white line of the abdomen. The gall bladder was removed into the wound and a cholecystectomy was performed from the bottom. For final hemostasis in the gallbladder bed, 1 ml of hemostatic glue containing 8% zinc polyacrylate, 5% sodium alginate and 1.5% acetic acid was uniformly applied. After 29 seconds, a dense film forms, which is not washed off by the blood stream. It adheres well to the wound surface. When removing the film, bleeding does not resume. As a control, we stopped the bleeding by tightly pressing a tampon from a gauze napkin to the wound surface. Bleeding stopped in 350 seconds.

Claims (1)

A hemostatic agent based on alginate, characterized in that it is a solution containing sodium alginate 10-100 g, 20-150 g of zinc polyacrylate and 1-30 ml of acetic acid in 1 liter of distilled water.