RU2250635C2 - Method for obtaining combined fodder premix - Google Patents

Abstract

FIELD: agriculture, combined fodder production.

SUBSTANCE: on should supplement grain filler being the constituent of target additives with a preparation obtained out of Mortierella gracile micromycete biomass according to the preset technology. The method enables to increase stability of combined fodder premix during storage period.

EFFECT: higher efficiency.

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Description

The invention relates to the feed industry.

A known method for the production of animal feed premix, comprising grinding the filler containing grain or products of its processing, and introducing into the filler the target additives in the form of biologically active substances and an antioxidant, the composition of which uses a lipid extract from biomass of micromycete Pythium gracile (RU 2138179 C1, 09/27/1999 )

The disadvantage of this method is to obtain the target product having insufficiently high storage stability.

The technical result of the invention is to increase the resistance of the target product to storage.

This result is achieved by the fact that in the method of manufacturing a feed premix comprising grinding a filler containing grain or products of its processing and introducing target additives into the filler, the composition of which uses a preparation from biomass of micromycete Pythium gracile, according to the invention to obtain a preparation from biomass of micromycete Pythium gracile it is subjected to sequential extraction with a non-polar extractant in a supercritical state, water, alkali, water, acid, water, alkali and water, and the first extra CT combine with solid residue.

The method is implemented as follows.

The dry biomass of Pythium gracile micromycete is extracted with a non-polar extractant, for example carbon dioxide or hexane, in a supercritical state. At this stage, the first extract is separated, which is used later in the preparation. Next, the biomass is sequentially extracted with water, alkali, water, acid, water, alkali and water. The solid residue obtained after completion of all the extraction steps listed above is combined with the first extract. Thus obtained drug is introduced into the crushed grain filler together with other target additives, in particular with biologically active substances and, if necessary, an antioxidant.

Pythium gracile micromycete biomass preparation consists mainly of higher polyunsaturated fatty acids with vitamin and antioxidant activity, as well as chitosan. Fatty acids contain significant amounts of arachidonic and eicosapentaenoic acids, which, in combination with chitosan, have especially high immunostimulating activity on grain. The composition of the drug does not include anti-nutritional, mutagenic, carcinogenic and toxic substances, which makes it possible to use it as part of compound feeds, and also does not include substances that can inhibit the immunostimulating activity of a mixture of chitosan with arachidonic and eicosapentaenoic acids, which increases the stability of the resulting premix during storage.

It should be noted that the treatment of grain with a preparation from the biomass of Pythium gracile micromycete obtained by the technology described above is effective at a drug consumption starting from 0.5 mg / t. At the same time, the amount of antioxidant in the premix should be about 0.1%. That is, as part of the premix, the preparation from the biomass of micromycete Pythium gracile can be used as a biologically active additive and antioxidant both independently and in combination with other components.

Example 1

The premix is prepared on the basis of corn grain, in which, as part of other components, a lipid extract from the biomass of micromycete Pythium gracile or a preparation from the same biomass, obtained according to the above technology using carbon dioxide as alkali, caustic soda and hydrochloric acid are added , in the amount of 1 kg / t. After 1 month of storage, the premix samples of the reference and experimental batches are contaminated with Penicillium funiculosum mold spores in an amount of 2 × 10 ¹² spores / g and incubated at 36 ° C for 10 days. Qualitatively, on the samples of the reference premix, mold growth was detected; on the samples of the experimental premix, it was not detected.

Example 2

The premix is prepared using rice flour and crushed oat grains as a filler, into which a mixture of butyloxytoluene with a lipid extract or Pythium gracile micromycete biomass prepared using hexane as a non-polar extractant, as an alkali of ammonium hydroxide and propionic acid, is introduced , at the expense of the last 1 mg / t. Check for mushroom resistance is carried out according to example 1, but using Aspergillus niger mold spores. The result coincides with example 1.

Thus, the proposed method allows to obtain the target product with higher stability during storage.

Claims (1)

A method of manufacturing a feed premix comprising crushing a filler containing grain or products of its processing and introducing target additives into the filler, which use a preparation from biomass of micromycete Pythium gracile, characterized in that it is subjected to sequential extraction with non-polar to produce a preparation from biomass of micromycete Pythium gracile supercritical extractant, water, alkali. water, acid, water, alkali and water, and the first extract is combined with a solid residue.