RU2250257C1 - Microorganisms trapping device - Google Patents

Abstract

FIELD: hygiene and sanitation; devices for trapping of microorganisms.

SUBSTANCE: the invention is dealt with the field of hygiene and a sanitation and intended for quantification of microorganisms in the air of premises. The microorganisms trapping device contains a conical container, in the lower part of which a trapping liquid is poured. In the upper part of the container under a grid there is a filter mounted with the help of an elastic tightening ring, which is pressed by a cover of the container. In the middle part of the conical container an aperture of a small diameter is made at an acute angle, for example 45°, to its vertical axis. To the conical container in its middle part opposite to the indicated aperture there is a detachable head piece attached to if with the help of a threading and supplied with an auxiliary filter, that is fixed in it with the help of the elastic tightening ring and the cover. The invention ensures an increased quantity of microorganisms singled out from air in premises and so the degree of their air purification.

EFFECT: the invention ensures an increased quantity of microorganisms singled out from air in premises and an increased degree of their air purification.

2 dwg

Description

FIELD OF THE INVENTION

The invention relates to hygiene and sanitation, is intended to determine the number of microorganisms in indoor air and can mainly be used in animal husbandry in order to timely detect pathogens and conduct a complex of anti-epizootic measures.

State of the art

A known device for trapping microorganisms from the air, in which a vacuum chamber is installed in the form of a cylinder, turning into a cone. The inner cone of the cylinder corresponds to the hollow truncated cone included in it, the diameter of the base of which is equal to the inner diameter, and the diameter of the truncation holes is smaller than the diameter of the cylinder. The cameras communicate through the holes. The cylinder of the vacuum chamber is rigidly connected to the cover, and reflectors in the form of equal convexities are located opposite to each other in different cutting planes on the inner surface of the cylinder. This embodiment of the device increases the intensive mixing of air with liquid and its throughput (see AS No. 341835, M. class. With 12 k 1/00, 1972).

The known device, however, does not provide the necessary accuracy of the studies, since the possibility of removing microorganisms not retained in the liquid from the vacuum chamber is not excluded. In addition, it is possible for microorganisms to directly enter the liquid absorber through the inlet of the suction chamber after taking an air sample, which does not provide reliable data on the advisability of preventive measures.

Also known is a device for trapping microorganisms, including a container for trapping liquid, a nozzle and a lid with a hole (V. Kiktenko and others. Bacterial aerosols and methods for their research in sanitary microbiology. M., 1968, pp. 140-153).

However, this device is difficult to operate.

The closest in technical essence and the achieved positive effect is the trap of microorganisms from air, adopted by the authors as a prototype, which consists of a container having a conical shape, a trapping liquid is poured into the lower part, and a filter is installed in the upper part under the mesh and with an elastic insert pressed by a lid with a hole. In the middle part of the cyclone, at the level of the surface of the trapping liquid, there is a jet located at an angle to the liquid. This embodiment of the device ensures the separation and retention of microorganisms in the trapping liquid, on the walls of the cyclone and on the filter (see AS No. 941422, class C 12 N 1/00).

However, the known device does not have a high level of release of microorganisms from the air.

Disclosure of invention

The technical result, which can be obtained using the present invention, is to increase the number of selected microorganisms from the air and the degree of its purification.

The technical result is achieved using a device for trapping microorganisms, consisting of a cone-shaped container with a lid, while a filter is installed in the upper part of the container, and a small diameter hole is made in the middle of the conical container at an acute angle to the vertical axis of the conical container, while it is equipped with a removable nozzle with a cover and an auxiliary filter, the latter is made with the possibility of fixation with the help of a sealing ring and cover.

Brief Description of the Drawings

Figure 1 shows a longitudinal section of a device for trapping microorganisms.

Figure 2 shows a sketch of the grids 3.

The implementation of the invention

The device for trapping microorganisms consists of a cone-shaped container 1, in the lower part of which a trapping liquid 2 is poured, and a working filter 4 is installed in the upper part under the mesh 3, and using an elastic sealing ring 5, which is pressed by the cover 6. The upper part of the cover 6 is made in fitting form. In the middle part of the cone-shaped container 1, a hole of small diameter 7 is made at an acute angle, for example, 45 ° to the vertical axis of the cone-shaped container 1. To the conical container 1 in its middle part opposite the hole 7, a removable nozzle 8 is equipped with a threaded connection, equipped with an auxiliary filter 9, which is fixed in an additional removable nozzle 8 using an elastic sealing ring 10, and the cover 11.

The device for trapping microorganisms works as follows: for research, the cone-shaped container 1 is filled with the trapping liquid 2 to the level of the hole 7. The filter 4 is installed in the upper part of the container 1 using a mesh 3, a rubber sealing ring 5, which is pressed against the container body 1 by a cover 6. The electric aspirator is connected to the cover 6 using a flexible rubber hose (not shown). Turn on the electric aspirator (not shown) in 3 l / min mode. creates an air pressure in the tank 1, which ensures the flow of test air into the cone-shaped tank 1 through the hole 7. Due to the small diameter of the hole 7, the air flow rate in accordance with the Bernoulli equation for continuity of the medium increases significantly compared to the speed of air movement other parts of the air trajectory. The increased air flow velocity and the direction of this flow, determined by the position of the axis of the hole 7, create conditions under which aerosol particles are separated from the surface of the trapping liquid, to which dust parts and microorganisms in the intake air adhere. Microorganisms that have not separated from the air are deposited on the walls of the cone-shaped container 1 and on the lower surface of the working filter 4.

After passing a certain amount of air through the device, which corresponds to the specified operating time of the electric aspirator, a removable nozzle 8, equipped with an auxiliary filter 9, is fixed to the cone-shaped container 1, which is fixed in the removable nozzle 8 using the rubber sealing ring 10 and cover 11. The device turn over, as a result of which the inner surface of the cone-shaped container 1 is washed by the catching liquid 2. After that, the filtering liquid is filtered 2 hours es working filter 4 through the capture liquid pumping elektroaspiratorom 2. As a result of filtration of the trapping liquid 2, the microflora is concentrated on the surface of the filter 4. Due to the presence of a removable nozzle 8 equipped with an auxiliary filter 9, it becomes possible to perform the processes of filtering the trapping liquid 2 and concentrating microorganisms on the surface of the working filter 4 without using a sterile box in non-laboratory conditions, since the auxiliary filter 9 serves as an obstacle for microorganisms contained in the air entering the cone-shaped container 1 in those ix since the filtering process of the capture liquid 2 through the working filter 4. The cap 6 is unscrewed, the working filter 4 is aseptically removed from the apparatus and colored. Further counting of microorganisms is performed using a microscope and an ocular mesh (not shown) under off-laboratory conditions.

The invention in comparison with the prototype and other known technical solutions has the following advantages:

- this device allows you to conduct research without the use of a sterile box in off-laboratory conditions;

- the device has a high efficiency of trapping microorganisms, as it allows for dual air filtration: at the inlet through the liquid and at the outlet through the filter;

- the proposed catcher is easily sterilized by all conventional methods;

- this device is cheap and easy to manufacture and operate.

Claims (1)

A device for trapping microorganisms, containing a cone-shaped container with a lid, a filter installed in the upper part of the container and a small diameter air inlet opening at an acute angle to the vertical axis of the container in its middle part, characterized in that it is equipped with a removable nozzle with a lid and an auxiliary filter installed in it with an o-ring and cover.

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