RU22478U1 - CUVETS FOR GROWING MICRO-ORGANISMS - Google Patents

Abstract

A cuvette for growing microorganisms made of glass, containing a sealed cover into which two tubes for aeration of the medium are mounted, characterized in that the cuvette has double walls for the circulation of warm liquid between them and a supply and outlet tube for the liquid to enter and exit the cuvette her.

Description

CUVETS FOR GROWING MICRO-ORGANISMS

The utility model relates to the design of laboratory devices used in microbiology and biochemistry for the cultivation of microorganisms in liquid nutrient media and photometric control.

Famous fermenter (AS SSSS No. 1650692, C 12 M, priority 10.09.87, publ. 05.23.91 ,, BI No. 19) used to control the concentration of microorganisms suspended in a liquid medium containing gas, where the optical density culture fluid is measured in a flow cell through which light passes from the light source to the receiver.

The disadvantages of this fermenter is the adsorption of microorganisms on the supply tubes, the phenomenon of "cold shock, which consists in changing the dynamics of growth of the culture with a sharp change in temperature at the time of passage of the liquid nutrient medium through the outlet tubes, which distorts the experimental results.

The closest in essence to the proposed cell is a laboratory fermenter (AS No. 1731805, C 12 M, priority 03.05.90, publ. 05/05/92, BI. No. 17) containing a glass beaker with a lid, a light source installed in the lid, a photoresistor located under the bottom of the glass, and a magnetic stirrer, where the light source is enclosed in a vertical tube that serves to supply purified air, the lower output section of which is located at the bottom of the glass and has a side cutout for uniform exit of air bubbles.

The disadvantages of the fermenter is the inability to maintain a constant temperature regime - a necessary condition for the growth of microorganisms.

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IPC: C 12 M

The purpose of the utility model is to create optimal conditions for the growth of microorganisms in a small amount of nutrient medium.

This goal is achieved by the fact that the inventive cell for growing microorganisms in a small amount of liquid nutrient medium and measuring the optical density of the culture fluid, made of glass, containing a sealed lid in which two tubes for aeration of the medium are mounted, has double walls for circulation between them warm liquid, and bringing, uyu and outlet tubes for the entry of this liquid into the cell and exit from it.

The utility model is illustrated by graphic material, where:

Figure 1 shows a general view of the cuvette in section AA, where the cuvette contains a glass case 1, a sealed cover 2, in which two tubes 3, 4 for aeration are mounted; The utility model case has double walls: outer 5 and inner 6, in the space between which the liquid circulates, it enters; through the drive; through its tube 7 and through; through the outlet tube 8.

The utility model works as follows: in a cuvette in a small amount of nutrient medium (5-6 ml) microorganisms grow. The temperature regime is maintained due to the circulation of warm liquid between the double walls of the cell. Aeration of the medium is carried out through the inlet tube with various gases (air, oxygen, ozone - depending on the tasks). Gases exit through the exhaust pipe. The cuvette is placed in a spectrophotometer, where the optical density of the culture fluid is measured, which allows us to judge the phases of culture growth, the intensity of culture propagation under the influence of various gases in dynamics.

We conducted a series of experiments on the cultivation of E. coli strain HB-101 using the proposed cell: in liquid

culture medium was introduced into the LB nutrient medium, after which, under conditions of aeration with atmospheric air, the growth dynamics of the culture was studied on a spectrophotometer at a wavelength of A, 600 for several hours. As a result of processing the obtained data, a microorganism growth curve is constructed, on which the latent growth phase and the logarithmic growth phase of microorganisms are clearly visible. The measurement results at a wavelength of A, 600 nm are presented in the graph - figure 2.

The advantage of this utility model is the ability to maintain a constant temperature during the experiment, which allows you to create optimal conditions for the growth and reproduction of microorganisms, as well as long and continuous measurements of optical density, which extends the capabilities of the experiment.

Claims (1)

A cuvette for growing microorganisms made of glass, containing a sealed cover into which two tubes for aeration of the medium are mounted, characterized in that the cuvette has double walls for the circulation of warm liquid between them and a supply and outlet tube for the liquid to enter and exit the cuvette her.