RU2237718C1 - Method for producing alcohol from waste - Google Patents

Abstract

FIELD: alcoholic industry.

SUBSTANCE: invention relates to producing alcohol. Method involves preparing raw, its digestion, cooling of digested mass, saccharifying mass with α-amylase, glucoamylase, xylanase, cellulase, cooling the prepared must, fermentation of must and isolation of alcohol. Glucoamylase, α-amylase, xylanase and cellulase are added as the complex enzyme preparation with the following activity of components, U/g, not less: amylolytic, 250; glucoamylase, 500; xylanase, 600; cellulase, 300. Mass is saccharified at temperature 60^oC for 2 h and fermentation of must is carried out at temperature 35^oC for 72 h.

EFFECT: increased yield of alcohol, simplified method.

4 cl, 1 tbl, 1 ex

Description

The invention relates to the production of alcohol from waste, in particular from waste of plant origin, including food production.

A known method for the production of alcohol from plant materials, providing for the processing of raw materials, in particular grain, with protosubtiline at the rate of 14 units / g of protein of grain, followed by saccharification, fermentation of the obtained saccharified wort, distillation and rectification of alcohol (RU 2143001 C1, C 12 P 7/06, published on December 20, 1999).

The disadvantage of the described method is that in plant materials along with protein and starchy carbohydrate contains a significant amount of non-starchy carbohydrates - hemicelluloses and cellulose.

The use of the above enzymes in the described method does not allow the use of non-starchy carbohydrates, which reduces the efficiency of the fermentation process and ultimately affects the yield of the finished product - alcohol.

The closest technical solution to the proposed method is a method for the production of alcohol, which involves heating the raw material to 50 ° C, hydrolyzing it with amylase at the rate of 3-4 units. AC / g conditional starch, heating the mixture to 100 ° C, cooling to 55 ° C, keeping the mixture at this temperature for 1 hour while simultaneously feeding at this stage a complex enzyme preparation containing glucoamylase and bacterial amylase and a complex enzyme preparation containing cellulase, xylanase and pentosanase. The mixture is aged until a wort with a solids concentration of 25% is obtained. Then the wort is cooled to 24 ° C, fermented, and alcohol is isolated (RU 2127760 C1, C 12 P 7/06).

The disadvantage of this method is its complexity, since enzymes are added at different stages of alcohol production, and natural products are used as raw materials.

The objective of the proposed technical solution is the disposal of waste of plant origin, including food production.

The problem is solved due to the fact that in the method for the production of alcohol, which involves preparing raw materials, boiling, cooling the boiled mass, saccharifying the mass with amylase, glucoamylase, xylanase and cellulase, cooling the wort, fermenting the wort and isolating alcohol, according to the invention, amylase, glucoamylase, xylanase and cellulase is administered in the form of a complex enzyme preparation having activity of at least units / g: amylolytic - 250, glucoamylase - 500, xylanase - 600, cellulase - 300, while saccharified e mass is carried out at a temperature of 60 ° C for 2 hours and the fermentation of the wort is carried out at a temperature of 35 ° C for 72 hours. In addition, plant-based food waste is used as raw material; from waste of plant origin, waste from the processing of fruits, berries, vegetables, corn cobs or mixtures thereof is used. In addition, waste from bakery, flour milling, wastes of catering enterprises (in particular, McDonald enterprises) are used as raw materials.

The invention is illustrated by an example.

Example

Ethyl alcohol is prepared in a batch process. The technological scheme of its production is presented in the drawing.

When preparing a batch, the ratio of raw materials to water varies depending on the type of raw materials used. The amount of water is selected taking into account the necessary consistency of the environment in a production environment.

The ratio of raw materials and water is presented in the table.

As a complex enzyme preparation, a preparation having activity, units / g is used:

Amylase 250

Glucoamylase 600

Xylanase 300

Cellulase 500

in the quantities indicated in the table.

The effectiveness of the process is judged by the values of the amount of dry soluble substances of the wort, titratable acidity of the mash and the content of ethyl alcohol in the mature mash.

The alcohol content in the mash was determined by distillation, followed by its establishment in the distillate by the aerometric method.

The results are presented in the table.

It can be seen from the table that the use of the developed complex enzyme preparation in the production of ethyl alcohol from waste of plant origin, including food waste and their mixtures, under certain conditions, allows to increase the yield of alcohol in comparison with the production of alcohol by classical technology.

Important advantages of the proposed method for the production of alcohol in comparison with the known ones is the disposal of food waste and the use of a single-stage saccharification of a complex enzyme preparation consisting of amylase, glucoamylase, xylanase and cellulase.

Claims (4)

1. The method of production of alcohol, including the preparation of raw materials, boiling, cooling the boiled mass, saccharification of the mass of α-amylase, glucoamylase, xylanase, cellulase, cooling the resulting wort, fermentation of the wort, the allocation of alcohol, characterized in that α-amylase, glucoamylase, xylanase and cellulase is administered in the form of a complex enzyme preparation having activities of at least units / g: amylolytic 250, glucoamylase 500, xylanase 600, cellulase 300, while saccharification of the mass is carried out at a temperature of 60 ° C for 2 hours, and sat wort cultivation is carried out at a temperature of 35 ° C for 72 hours 2. The method according to claim 1, characterized in that the food waste of plant origin is used as raw material. 3. The method according to claim 2, characterized in that the waste from vegetable origin uses waste from the processing of fruits, berries, vegetables, corn cobs or mixtures thereof. 4. The method according to claim 1, characterized in that the raw materials used are waste from baking, flour milling, waste from catering establishments or mixtures thereof.