RU2214595C2 - Method for noninvasive detecting the presence of body xenobiotics and device for its implementation - Google Patents

Abstract

FIELD: medicine, medicinal technique. SUBSTANCE: the present innovation deals with bioinformation-type molecular- clinical diagnostics to detect the presence of body xenobiotics in homeopathic concentration (potency) considered to be noninvasive and pain-free for a patient, moreover, it is easily performed and reproduced for researcher. The method is carried out by conducting comparative measurements of electric skin conductivity in biologically active point with the help of device based upon conducting autonomic resonance test. Block-scheme is suggested to implement this method. This innovation enables to increase sensitivity, accuracy and selectivity in detecting harmful agents in human and animal bodies at the smallest concentrations (homeopathic level). EFFECT: higher efficiency. 5 cl, 1 dwg, 3 ex

Description

 The invention relates to the field of medicine and medical technology, in particular to bioinformatic model-clinical diagnosis, and is intended to detect the presence of xenobiotics in the body.

 Xenobiotic (KB) (from the Greek "xenos" - alien, "bios" - life) - a foreign substance, a foreign compound is a substance that this body can not use to produce energy, nor to build the components of a living cell. Environmental pollutants: industrial emissions, automobile exhaust, livestock waste, various aerosols, fertilizers, pesticides, detergents, food preservatives and dyes, as well as some types of medicines, drugs, nicotine, etc. into the human body, not only because they are associated with their direct use, but also with food, water and air [1]. The detection of xenobiotics in the body in most cases is laborious and invasive for the patient.

 There are various methods for detecting KB substances in the human body, consisting in instrumental chemical analysis (chromatography, spectroscopy, etc.) of blood, urine or saliva samples [2]. However, the performance of such analyzes is associated with great complexity and considerable time costs, including obtaining and preparing samples, as well as the need to collect them for analysis either immediately after the xenobiotic enters the body, or not later than a few hours or, in extreme cases, a day. In addition, chemical analysis methods do not allow the retrospective detection of the presence of xenobiotics in the body, for example, after several weeks or months, and the measuring equipment does not differ in portability and speed.

 At present, bioinformation methods of examining a person are becoming more widespread in order to determine his state of health, to identify various pathological conditions in him, without resorting to taking biological fluids and tissues, which is a safer and more reliable diagnostic measure for the patient [3].

 The study of patent information showed that the diagnostic examination of patients using special automatic (in particular, bioresonance) devices in recent years has become very relevant (patents of the Russian Federation 2070405, 1996, 2070406, 1996). Various electrophysiological devices are also known for studying the state of an organism by the electrical conductivity of the skin in the area of acupuncture points (see, for example, Pat. RF 2137458, 1999).

 Currently, using automatic devices connected to a computer, it is even possible to remotely control the dose of an injected drug from a syringe (see US Pat. No. 5,792,117, 1998), as well as remotely monitor the patient's condition by determining the necessary marker indicators in the body (US Pat. 6270455, 08/07/2001).

 However, the known adaptive bioinformation methods and devices do not allow to determine the presence in the body of a foreign substance (xenobiotic), for example, such as a drug, nicotine, pesticide, herbicide, anabolic drug, semi-synthetic antibiotic (e.g. cephaloridin), etc., which is in the body in a very small dilution (homeopathic level).

 The closest to the claimed can be considered the development, which is disclosed in Russian patent 1745100, 1987, which describes the operation of the device for diagnosing the state of the body by studying the state of biologically active points. The invention describes the detection of a foreign agent (in particular, a toxic dipteroid) by measuring the electrical conductivity of the skin at biologically active points (BAP). In the presence of a foreign agent, the electrical conductivity of the skin in the BAP area changes.

 However, this method does not allow to determine in vivo the presence within the body of a foreign agent in a very low concentration.

 The technical task of the present invention is to provide a method for detecting the presence of xenobiotics in the body in trace (informational) concentration, the method should be non-invasive, painless for the patient, fairly easy to implement and reproducible for the researcher.

 The problem is achieved in that, first of all, for carrying out such a method it is necessary to identify a special BAT, the so-called reproducible point, the electrical conductivity of which determines the presence of a xenobiotic. In addition, additional information about the determined xenobiotics in the form of their spectral characteristics corresponding to various dilutions of the homeopathic level, the so-called test pointers, is added to the measuring circuit of the bioresonance device. When the wave spectra of the “external xenobiotic”, taken from test pointers, interact with the body present (desired), a resonance phenomenon occurs, which is reflected in the change in the measured parameters of electromagnetic signals emitted by the body (for example, ECG, EEG, etc.). We found that the most convenient way is to measure the dynamics of changes in the electrical conductivity of the skin at acupuncture points or BAP of the skin. For measurements, both absolute and relative indicators can be used, consisting in a change in the conductivity of the skin in a certain direction (its decrease or increase). Such measurements make it possible to record the presence of xenobiotics in the body. If it is present in the body, the electrical parameters of the skin, measured in the BAP before and after comparing them with the KB marker from the test pointers located in the external measuring circuit, are different. Otherwise, these changes are absent. Measurements are taken at any BAP or acupuncture point that are associated with an organ that does not have pathological disorders, i.e. reproducible measurement point, which is detected by the so-called "pumping" method.

 The method according to the invention is as follows.

 The method is based on a vegetative resonance test (ART). First, the patient selects the BAP or acupuncture point, which is designated as the measurement point (TI), choosing among them the reproducible TI. The reproducible point is a TI, during repeated measurement of which the same measured values are obtained by the "pumping" method.

 If a return to the primary result does not occur with subsequent pressures on the TI, then this indicates a violation in the organs or systems associated with the point, and it cannot be used for measurements of ART. For example, in a patient suffering from allergies, the 1st meridian point of the allergy described by R. Voll will be irreproducible, since when measuring conductivity repeatedly at this point, pumping shows a decrease in the values on the scale of the device relative to the initial level.

The method of "inflation" is performed as follows:
- pressure is applied to the selected TI using a special probe equipped with an elastic plate;
- the inflation process should not exceed 3-5 seconds;
- the probe pressure per point should not exceed 100-200 grams (1-2 newtons);
- after reaching the maximum value of the measured value in the TI, for example, 40 arbitrary units (cu) of the scale, the probe pressure decreases without tearing off the tip of the probe of the active electrode from the point (at this time, the values of the indicators on the scale of the device decrease). Then the probe pressure is gradually increased again (inflation). If the initial value is 40 cu the scale after this pumping is again achieved, then TI is considered reproducible, if not, then this indicates the presence of pathology in the organs and systems associated with this TI. Such a point is considered irreproducible and cannot be used for measurements;
- pumping in time should be smooth (without jerking and spasmodic changes in pressure). If too much pressure is applied to the TI, then it may become unsuitable for measurement.

 Next, adjust the sensitivity of the device.

 To increase the resolution of the method on equipment that implements the invention, the scale of the measuring scale is expanded to 80 cu regardless of the baseline level of reproducible TI found for the study.

 After finding the reproducible TI and expanding the scale of the device to 80 o. E. the test drug is introduced into the measuring circuit, which marks the presence or absence of a xenobiotic substance in the patient’s body, and then the measurement is repeated by pumping. If the value obtained on the scale of the device is less than 80 cu, then the test is considered positive (yes, there is a CB in the body), if it returns to the initial level, it is negative (no, there was no fact of a KB substance entering the body) .

 The invention in terms of the method is illustrated by the following examples.

 EXAMPLE 1. 20 of the most commonly used substances with narcotic effects were selected: opium, heroin, morphine, promedol, narcotine, codeine, papaverine, thebaine, amphetamine, methamphetamine, MDMA, ephedrine, ephedrone, chlorpromazine, diazepam, ketamine, tradol, clonidine, cocaine and amitriptyline, which are represented in homeopathic potencies - D3, D4, D5, D6, D8, D10, D12, D15, D30, D60, D100 and D200 as test pointers. It was diagnosed 670 people who used drugs at various times until the moment of measurement, which made it possible to establish the accuracy of determining the presence of narcotic substances in the body of 97.5% compared with a group of intact subjects (230 persons).

 In the study of the presence of various narcotic substances in the body, it was found that using the method and device developed by us, it is also possible to determine the approximate dates of a person's use of a drug.

 The results of the resulting pattern are presented below.

Homeopathic Potency of a Narcotic Substance - Estimated Duration of Substance Use
D3 - 1 day
D4 - 2, 3 days
D5 - 4, 5, 6 days
D6 - 7, 8, 9, 10 days
D8 - 11, 12, 13, 14, 15, 16 days
D10 - 21 days
D12 - 30 days
D15 - 3 months
D30 - 6 months
D69 - 9 months
D100, D200 - More than 1 year
EXAMPLE 2. Using the proposed method, where the anabolic drug methandrostenolone was used as test pointers for a number of homeopathic dilutions (D3 ... D200), a survey was conducted of 12 athletes who took it at different times before measurements. As a control group, 10 athletes who did not use anabolic drugs were used. The measurement accuracy in this case was 98.8%, and based on the value of homeopathic dilution, it was possible to determine the timing of the use of anabolics in the examined athletes.

 EXAMPLE 3. A survey was conducted of the contingent of people in the Krasnodar Territory working with plant protection products used in agriculture (pesticides and herbicides) used to spray rice and wheat fields, as well as orchards and vegetable gardens. As a result of measurements in 130 people who had contact with pesticides and herbicides, their presence was found to be 99.3% compared with a group of control persons that were not related to these chemicals by their nature of activity.

 Another aspect of the present invention is to provide a device for implementing a method for detecting KB.

 In particular, a device for electro-acupuncture diagnostics is known, with the help of which the state of an organism is detected by a change in the BAT electrical conductivity (e.g. WO 92/19155, 1992). This development is taken by us as a prototype, a device is described here, which is a design for diagnostics, comprising a block of conductivity measurement, consisting of a block of electrodes connected to an analog conductivity meter, which is connected to an analog-to-digital converter, the output of which is connected to a measurement and registration unit, including read-only memory and random-access memory connected to the processor via a data bus that is connected to the control bus, a pool t Control and display device, as well as block microresonant circuits and a control unit. This device is equipped with a calibration system to check its hardware.

 However, the known device does not allow to detect in vivo the presence in the body of foreign agents, the content of which in biological tissues and fluids is extremely low (concentration of the homeopathic level).

 The purpose of this development is to create such a device that allows you to determine the presence in the body of low, even trace, concentrations of KB substances such as drugs, pesticides, anabolics, cephaloridins, salts of heavy metals, etc.

 The technical result consists in the fact that using this device it is possible to increase the sensitivity, accuracy and selectivity of in vivo detection of harmful agents contained in humans and animals in extremely low concentrations (homeopathic level) using a non-invasive method.

 The drawing shows a functional diagram of a device containing an electrode unit 1, a switching unit 2, an amplification unit 3, an analog-to-digital converter 4, a control unit 5, and a measurement and recording unit 6 including a random access memory (RAM) 7, a parameter comparison unit 8, pressure adjuster 9, information storage unit 10, in which the wave characteristics of various xenobiotics (test pointers), processor 11, timer 12, data bus 13, input / output interface 14, control panel 15, display device 16, potassium block 17 are recorded edges, read-only memory device 18, buffer register 19, digital-to-analog converter 20 and bus 21 for communication with an external drive and a personal computer.

 The operation of the device is as follows.

 Before the start of the examination, the device is turned on and its functioning is monitored and the unit 6 is self-tested in accordance with the algorithm incorporated in it when using the calibration unit 17.

 Calibration is carried out according to standard methods.

 After calibrating the device, the doctor switches to the diagnostic mode, for which measurements of the electrical conductivity of the skin in the BAP area are performed. To do this, programmatically, from the control panel 15, the selection of the required pairs of electrodes (passive and active) is carried out. The doctor gives the patient an indifferent (passive) electrode in the patient’s hand, and the active electrode leads to the acupuncture point to obtain an analog measured signal. The active electrode is made in the form of a probe, which is an elastic plate on which a strain gauge is glued. When pressing the probe on the skin surface, the force value is fixed by a strain gauge and can be measured in the form of an electrical signal.

 Since it is necessary to provide a certain pressure in the measured BAT with a probe (force 1-2 N, referred to the area of the probe end face), the doctor from the control panel gives a command to connect the pressure switch 9 and timer 12, which is set for a certain period of time (3-5 s) . When pressure is reached by the probe on the acupuncture point corresponding to the set within a certain period of time, the unit 8 comparing the parameters disables the pressure setter and timer. The received analog signal after amplification in block 3 and its digital conversion in the analog-to-digital converter 4 through the input-output interface 14 and the data bus 13 is recorded in RAM 7 and fed to the processor 11 for processing. In accordance with the program laid down, the signal is processed and appears in the form of a graphic image on the display device 16.

 Upon reaching a stable state of this image (i.e., when the readings of the electrical conductivity values are repeatedly repeated, which indicates the reproducibility of this acupuncture point), the doctor, using the control panel 15, starts a program, according to which a command is issued, which is received via the input-output interface 14 to connect the corresponding test pointer from the information storage unit 10, in which the wave characteristics of a specific xenobiotic are recorded in a specific homeopathic medicine enii to block one of the electrodes (via the input-output interface output 14, buffer register 19 and digital to analog converter 20).

 If after exposure to the acupuncture point of the test pointer connected to it on the display device 16, the readings do not change, this indicates the absence of patient response to this xenobiotic, i.e. this KB in the patient’s body is absent. The doctor gives a command to connect the next test pointer, etc.

 Enumeration of connections of test pointers is carried out before resonance, the reaction to which is manifested in a change in the conductivity index. This indicates that this xenobiotic is present in the patient's body.

 Since the readings of RAM 7 are overwritten into read-only memory 18, all data in the device is stored. On the bus 21 of the I / O interface 14, they can be transferred to an external network or to a personal computer. Through the same bus, information about test pointers can be added and edited.

 Thus, the present invention allows to determine the presence in the body of foreign substances (xenobiotics) located in tissues in negligible (up to trace and informational) concentrations using a non-invasive, very fast and reproducible method. The invention will allow to further control the state of human health, as well as the level of environmental pollution.

LIST OF REFERENCES
1. Zakharov V.M. Environmental health: concept. - M., 2000.

 2. Reference biochemist. - M.: Mir, 1991, 544 p.

 3. Theoretical and clinical aspects of bioresonance and multiresonant therapy. - M .: Imedis, 1996-2001.

Claims (5)

 1. A method for non-invasively detecting the presence of a xenobiotic in the body by conducting comparative measurements of the electrical conductivity of the skin at a biologically active point using a device based on a vegetative resonance test, characterized in that the comparison unit about xenobiotics is introduced into the measuring unit in the form of its spectral characteristics, corresponding to various homeopathic dilutions (test pointers), before conducting the initial measurement of electrical conductivity (EP), choose the biologically active measuring point (TI) is performed by pressing the probe equipped with an elastic plate until the desired pressure is fixed, the EP measurements of the test point with variable pressure are repeatedly repeated (the "pumping" method), and if the point is saved during repeated measurement, the point is considered reproducible, Then, skin EPs in the region of the selected TI are compared before and after the introduction of information on the xenobiotic test-pointer, and when the obtained value of the EP deviates after repeated measurement, the presence of xenobiotic, and while maintaining the initial value of EP after repeated measurement - the absence in the body of the desired xenobiotic.  2. The method according to claim 1, characterized in that the test pointers contain spectral wave characteristics of xenobiotics such as drugs, herbicides, pesticides, anabolic drugs, heavy metal salts.  3. The method according to PP. 1 and 2, characterized in that the detection of the presence of xenobiotics in the body in a specific homeopathic dilution allows us to judge the limitation periods of its ingestion.  4. A device for non-invasively detecting the presence of a xenobiotic in the body, containing a block of electrodes, an analog-to-digital converter connected to the input of the measuring and recording unit, including a processor, operational and permanent memory devices, the inputs and outputs of which are connected to the data bus, the input-output interface , an information storage unit, a calibration unit, a control panel and a display device, as well as a control unit, characterized in that a buffer register and a digital-to-analog converter are entered into it, bl approx. electrodes are connected to the analog-to-digital converter via switching and signal amplification units, the active electrodes in the electrode block are made in the form of elastic plates with strain gauges, and an additional parameter comparison unit, a pressure regulator and a timer are added to the measurement and registration unit, while the outputs of the control unit connected respectively to the control inputs of the switching unit, analog-to-digital converter, buffer register, digital-to-analog converter and input-output interface, information input it is connected to the output of the analog-to-digital converter, the output is connected through the buffer register and the digital-to-analog converter to the electrode block, and the inputs / outputs - to the inputs / outputs of the data bus, to which the inputs and outputs of the parameter comparison unit, the information storage unit and the calibration unit are connected, as well as a timer and pressure setter, the output of which is connected to the input of the parameter comparison unit, and the control panel and display device are connected to the input-output interface.  5. The device according to claim 4, characterized in that the input-output interface has a communication bus with an external information storage device or personal computer.