RU2209433C2 - Method for determining indications to intra-articular administration of chondroprotector agents based on cartilage glycosoamines hydrolysate - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: method involves adding chondroprotector agent based on cartilage glycosoamine hydrolysate to Bacto-Agar Typ USA solution and mixing. The solution is poured over glass plate and gel layer is produced. Synovial fluid is dropped into holes after having perforated the gel layer and hold in crystallizer. Coloring taking place, precipitate rings are identified. Rings availability indicates contraindications to intra-articular administration of chondroprotector agents based on cartilage glycosoamine hydrolysate. EFFECT: high accuracy in predicting in vitro autoimmune process development; avoided hypersensitivity phenomena. 1 dwg

Description

 The invention relates to medicine, in particular to laboratory diagnostics of autoimmune processes in joints during osteoarthritis and can be used to determine indications for intra-articular administration of chondroprotectors based on glycosaminoglycans of articular cartilage hydrolysates.

 It is known that as a result of degenerative changes in the cartilage, the destruction of the main substance of the connective tissue develops, it becomes possible to release its various components (proteoglycans, collagen, etc.) [1, 2]. Released proteoglycans, decay products of chondrocytes, collagen, acting as autoantigens, are involved in the immunopathological process and contribute to its further development. Since chondroprotectors containing glycosaminoglycans are used as the basic therapy for the treatment of osteoarthritis, an artificial increase in the concentration of glycosaminoglycans in the human body provokes the development of immunological reactions to them, and, consequently, the activation of the process. The heterogeneity of the immunopathological reactions that underlie the genesis of the disease can explain the need for an individual approach to the treatment of patients with osteoarthritis by the method of intra-articular administration of chondroprotectors based on glycosaminoglycans of articular cartilage hydrolysates. The indirect version of the solid-phase enzyme-linked immunosorbent assay adopted as an analogue, described in various literary sources, is highly sensitive and specific.

 However, it is used to determine the level of antibodies to cartilage glycosaminoglycans in blood serum, which does not allow to determine the exact localization of the autoimmune process.

 In addition, this method requires good technical equipment of a medical institution, training of high-class specialists.

 At the same time, a unified laboratory test has not been developed to determine indications for intra-articular use of chondroprotectors based on cartilage glycosaminoglycan hydrolysates.

 The objective of the present invention is to develop a method for determining indications for intra-articular administration of chondroprotectors based on cartilage of glycosaminoglycan glycosaminoglycans in vitro, which allows in vitro prediction of the development of an autoimmune process in the joint and prevents the occurrence of hypersensitivity reactions during intra-articular administration of chondroprotective preparations based on hydrolyzate of glycosaminoglycans x.

 The problem is solved in that in the method for determining the indications for intra-articular administration of chondroprotectors based on cartilage glycosaminoglycan hydrolyzate into the prepared Bacto-Agar Tour USA solution, the chondroprotector based on cartilage glycosaminoglycan hydrolyzate is mixed, then the resulting solution is poured onto a glass plate and a gel layer is obtained, and a gel layer is obtained, and synovial fluid is instilled into its perforations, kept in a crystallizer and, when stained, the formation of precipitate rings, the presence of which rykh determines contraindications for intraarticular use of chondroprotectors based on cartilage glycosaminoglycan hydrolyzate.

 The patented invention is illustrated by a detailed description of the method and is illustrated by an example of determining indications for intra-articular administration of chondroprotectors.

 The method is as follows.

 Receives punctate synovial fluid. A Bacto-Agar Tour USA solution is prepared. A hydrolyzate of glycosaminoglycans or a chondroprotector are introduced into it. The resulting solution is poured onto glass 90x120 mm and left to solidify the gel. After that, perforation of the gel layer is performed and synovial fluid is introduced into the obtained wells. Glasses with gel are left in a humid chamber for 24 hours. To visually assess the results, stain the gel with Amido Schwartz. The presence of precipitation rings in the gel is a contraindication for intraarticular administration of chondroprotectors.

 The proposed method is easy to use, for its implementation does not require the use of expensive research equipment. It can be used in the laboratory of any technical equipment.

 In addition, the use for diagnostic purposes of the synovial fluid of a particular patient allows you to accurately determine the localization area of the autoimmune process.

Sources of information
1. Kovalenko EA The relationship of the level of antibodies to cartilage glycosaminoglycans in patients with osteoarthritis with the effectiveness of treatment with chondroprotectors. Abstract of diss, for competition. student Art. Ph.D., Volgograd, 1997, 23 pp.

 2. Babaeva A.R. The clinical and pathogenetic significance of the study of serum glycosaminoglycans and antibodies to them in diseases of extraarticular soft tissues. Abstract of diss. for a job. part MD, Volgograd, 1997, 45 p.

Claims (1)

 A method for determining indications for intra-articular administration of chondroprotectors based on cartilage glycosaminoglycan hydrolyzate, in which a chondroprotector based on cartilage glycosaminoglycan hydrolyzate is mixed into the prepared Bacto-Agar Tour USA solution, then the resulting solution is poured onto a glass plate and a gel layer is obtained after it is perforated into holes the synovial fluid is instilled, kept in the mold and, when stained, the formation of precipitate rings is identified, the presence of which determines the contraindication to intraarticular use of chondroprotectors based on cartilage glycosaminoglycan hydrolyzate.