RU2203542C2 - Method for detecting toxicity of media at vermicultivation - Google Patents

Method for detecting toxicity of media at vermicultivation Download PDF

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RU2203542C2
RU2203542C2 RU98117931/13A RU98117931A RU2203542C2 RU 2203542 C2 RU2203542 C2 RU 2203542C2 RU 98117931/13 A RU98117931/13 A RU 98117931/13A RU 98117931 A RU98117931 A RU 98117931A RU 2203542 C2 RU2203542 C2 RU 2203542C2
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media
vermicultivation
experiments
detecting toxicity
present innovation
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RU98117931/13A
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Russian (ru)
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RU98117931A (en
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Д.С. Потапов
Д.И. Стом
Е.В. Антонова
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Иркутский государственный университет
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Abstract

FIELD: biotechnology. SUBSTANCE: the present innovation deals with methods for evaluating fodder media usefulness for vermiculture and optimization of their composition. At detecting toxicity of media in case of vermicultivation one should apply a test-object as Enchytraeus albidus worm. The present innovation enables to use small volumes of substrates, carry out large number of experiments and conducts experiments under field conditions. EFFECT: higher efficiency of detection. 1 tbl

Description

Изобретение относится к области биотехнологии, а именно к способам оценки пригодности кормовых сред для вермикультуры и оптимизации их состава. The invention relates to the field of biotechnology, and in particular to methods for assessing the suitability of feed media for vermiculture and optimizing their composition.

Известен способ определения токсичности и пригодности новых субстратов для корма червей в лабораторных или полупромышленных условиях [1]. Опыты проводят в садках, куда помещают испытуемые субстраты и червей. Тестом служит выживаемость, интенсивность размножение животных, увеличение численности червей и коконов. Во всех рекомендациях по вермикультивированию дождевых червей особое место уделяется осторожности при внесении новых добавок в корм животных, чтобы исключить гибель червей и не снизить их физиологическую активность, вместе с тем избежать токсического действия субстратов не всегда удается. В качестве тест-организмов используют Eisenia foetida или красного калифорнийского червя. Недостатком способа является медленное размножение дождевых червей, потребность большого объема субстрата для выращивания и тестирования, частая гибель животных, длительный срок их созревания. Все это приводит к удорожанию процесса. A known method for determining the toxicity and suitability of new substrates for feed worms in laboratory or semi-industrial conditions [1]. The experiments are carried out in cages, where the test substrates and worms are placed. The test is survival, the reproduction rate of animals, the increase in the number of worms and cocoons. In all recommendations for vermicultivation of earthworms, special attention is paid to caution when introducing new additives into animal feed in order to exclude the death of worms and not reduce their physiological activity, however, it is not always possible to avoid the toxic effect of substrates. As test organisms, Eisenia foetida or California red worm is used. The disadvantage of this method is the slow propagation of earthworms, the need for a large volume of substrate for growing and testing, the frequent death of animals, the long term of their maturation. All this leads to a rise in price of the process.

Для устранения указанных недостатков предлагается использовать горшечного червя (Enchytraeus albidus) в качестве тест-объекта при биотестировании. To eliminate these drawbacks, it is proposed to use a potted worm (Enchytraeus albidus) as a test object during biotesting.

Основываются на таких его физиологических особенностях, как небольшие его размеры (длина тела 20-35 мм, толщина 2-3 мм), что дает возможность разводить и использовать при биотестировании в небольших объемах субстрата, высокая скорость размножения и плотность, до 100-150 тысяч на 1 м2, что также очень важно при проведении большого количества экспериментов, способность переносить температуры ниже 0oС, что позволяет проводить опыты в полевых условиях.They are based on its physiological features, such as its small size (body length 20-35 mm, thickness 2-3 mm), which makes it possible to breed and use it during biotesting in small volumes of substrate, high reproduction rate and density, up to 100-150 thousand per 1 m 2 , which is also very important when conducting a large number of experiments, the ability to tolerate temperatures below 0 o C, which allows experiments in the field.

Из приведенного примера видно, что горшечный червь обнаруживает по сравнению с Eisenia foetida и красным калифорнийским червем более высокую чувствительность к большинству токсических соединений. From the above example, it can be seen that, in comparison with Eisenia foetida and the California red worm, the potato worm is more sensitive to most toxic compounds.

Источники информации
1. Методика формирования лож и техника закладки маточного поголовья в субстрат. Условия содержания червей в ложах. - В кн.: Биоконверсия органических отходов в биодинамическом хозяйстве /Н.М. Городний, И.А. Мельник. М.Ф. Повхан и др. - Киев: Урожай, 1990. - С. 121-126.Sources of information
1. The method of forming the boxes and the technique of laying the brood stock in the substrate. The conditions of the worms in the boxes. - In the book: Bioconversion of organic waste in a biodynamic economy / N.M. Gorodny, I.A. Miller. M.F. Povhan et al. - Kiev: Harvest, 1990 .-- S. 121-126.

Claims (1)

Способ определения токсичности сред при вермикультивировании с использованием тест-объекта, отличающийся тем, что в качестве тест-объекта используют горшечного червя (Enchytraeus albidus). A method for determining the toxicity of media during vermicultivation using a test object, characterized in that a potato worm (Enchytraeus albidus) is used as a test object.
RU98117931/13A 1998-10-01 1998-10-01 Method for detecting toxicity of media at vermicultivation RU2203542C2 (en)

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RU2203542C2 true RU2203542C2 (en) 2003-05-10

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104770340A (en) * 2015-03-27 2015-07-15 华南农业大学 Method for determining stomach toxicity of oriental fruit flies by utilizing simple device

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Н.М. ГОРОДНИЙ И ДР. Биоконверсия органических отходов в биодинамическом хозяйстве. - Киев: Урожай, 1990, с.121-126. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104770340A (en) * 2015-03-27 2015-07-15 华南农业大学 Method for determining stomach toxicity of oriental fruit flies by utilizing simple device

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