RU2203067C1 - "rejuvenating" homeopathic antioxidant preparation - Google Patents

Abstract

FIELD: medicine, homeopathy. SUBSTANCE: preparation could be applied as curative-prophylactic remedy at diseases accompanied by activation of lipid peroxidation (LP) processes and accumulation of LP products in organs and tissues. It is suggested a homeopathic antioxidant preparation containing Ferrum iodatum, Carbo vegetabilis, Lachesis taken in equal ratios at C6 dilution, moreover, it is designed in the form of sugar granules. The present preparation has no side effects, contraindications and allergic reactions and could be applied in clinical and geriatric practice. EFFECT: improved results of rejuvenation. 1 cl, 3 ex

Description

 The invention relates to medicine, namely to homeopathy, and can be used as a therapeutic and prophylactic agent for diseases associated with activation of lipid peroxidation (LPO) processes and the accumulation of lipid peroxidation products in organs and tissues.

 Currently, free radicals and reactions with their participation are considered the cause of diseases such as atherosclerosis, cerebral, coronary and peripheral circulation disorders, diabetes mellitus and related complications, bronchopulmonary diseases, various intoxications, aging processes, etc. In this regard, increased interest in antioxidants, since it is known that the use of antioxidants can inhibit the activation of free radical reactions. Therefore, the challenge remains to develop new, effective, affordable therapeutic and prophylactic agents with antioxidant activity.

 The aim of the invention is the creation of a homeopathic remedy with antioxidant activity.

 This goal is achieved in that the product contains Ferrum iodatum, Carbo vegetabilis, Lachesis. All medicinal components are taken in equal proportions and when diluting C6.

 What is new in achieving the technical result is that a homeopathic remedy having a pronounced antioxidant activity is proposed. The tool helps to normalize free radical oxidative processes, which allows it to be used in practical medicine in the treatment of a wide range of diseases, the pathogenetic treatment of which requires the use of drugs that have antioxidant effects, as well as a prophylactic. The remedy can be prepared in homeopathic pharmacies.

 Thus, the proposed technical solution meets the criteria of the invention of "novelty", "inventive step", "industrial applicability".

 The proposed tool contains medicinal substances prepared by the method of homeopathy in potentiated doses and a neutral carrier. As medicinal substances, the product contains Ferrum iodatum, Carbo vegetabilis, Lachesis. Milk sugar is used as a neutral carrier. The tool is made in the form of granules. All medicinal components are taken in equal proportions and when diluting C6.

 Take the drug in 5-6 granules under the tongue (dissolve) 20 minutes before meals 3-5 times a day for 1-3 months.

 To prove the antioxidant effect, it was tested in model systems commonly used for this purpose.

 Example 1

 The effect of the antioxidant complex on the chemiluminescence of blood plasma was studied.

 It is known that the level of chemiluminescence of biological tissues and fluids is a reflection of mobile equilibrium, an objective integral indicator of the ratio of the intensity of lipid peroxidation processes and the activity of biological antioxidant systems of the body. Thus, the registration of chemiluminescence of biological tissues and fluids can be considered as a source of information about the biochemical processes taking place in them.

In the model system based on H ₂ O ₂ -stimulated chemiluminescence of blood plasma, the antioxidant activity of the proposed agent was determined.

 For analysis, the blood plasma of 10 healthy and 10 patients with bronchial asthma was used.

 To measure chemiluminescence, 0.05 ml of plasma was added to the luminometer cuvette and the volume of the reaction medium was adjusted to 1 ml with phosphate buffer with a pH of 6.5–9.0. The cuvette was placed in the luminometer chamber and 0.2 ml of a 0.1 M hydrogen peroxide solution was added, and then the maximum signal value was recorded (maximum glow - peak and time to peak).

The maximum value of chemiluminescence in the blood plasma of healthy people accounted for 10-15 seconds since the introduction of H ₂ O ₂ and amounted to 5.6 ± 0.5 srvc. units , while in the group of patients with bronchial asthma, these values were equal to 150.0 ± 18.2 srvc. units and accounted for 30-40 seconds from the start of the reaction. This indicates a high intensity of lipid peroxidation processes and reduced activity of bioantioxidant systems regulating this oxidation in these patients.

The introduction of the proposed agent into the model system from the plasma of patients with asthma in the entire range of used concentrations (1.2 • 10 ^-14 -1.2 mg / ml) led to a change in its chemiluminescence parameters - a decrease in luminous intensity by 45%.

 These results indicate that this tool is able to effectively intercept the active radicals present in the system.

 Example 2

 The antioxidant activity of the drug was determined using the Fenton reaction.

 Evaluated the antioxidant activity of the proposed drug by changing the intensity of the chemiluminescence of the Fenton reaction.

The complex under study was dissolved in distilled water, and immediately before the measurement, hydrogen peroxide was added at a final concentration of 0.4 mM. The Fenton reaction was started by the introduction of 10 μM Fe ²⁺ . To test the drug, 5 μg of the substance was taken per 1 ml of hydrogen peroxide solution. To the resulting solution, 1 ml of iron sulfate solution was added, and the maximum chemiluminescence amplitude of the Fenton reaction (A +) was measured. Then, the maximum glow amplitude of the same reaction was determined in the absence of the test substance (A ₀ ). The antioxidant activity of the drug was judged by parameter A, which was calculated by the formula
A = (A ₀ -A +) / A ₀ ,
where A ₀ - the maximum amplitude of the chemiluminescence of the Fenton reaction in the absence of the test substance,
A + is the maximum amplitude of chemiluminescence in the presence of the test substance.

 It is known that the larger the parameter A, the more pronounced the antioxidant properties of the test substance (for antioxidants A> 0.3).

The following data were obtained:
A + = 18.5 srvc units
And ₀ = 50 srvc. units
A = 0.63 conv. units

 Thus, the proposed homeopathic complex has pronounced antioxidant properties.

 Example 3

The proposed complex was used in the treatment of the following groups of patients:
Group 1 - 19 people with various forms of coronary heart disease (stable angina pectoris I - III functional class, post-infarction cardiosclerosis, heart failure),
2 group - 9 people with chronic liver diseases (chronic hepatitis),
Group 3 - 13 people with chronic obstructive pulmonary disease.

 Evaluation of the antioxidant activity of the drug was carried out on the basis of a comparative analysis of the dynamics of the LPO parameters: the content of diene conjugates (DC) and malondialdehyde (MDA) in blood plasma before and after treatment with the agent. The data obtained from 15 healthy donors are taken as the norm.

 Prior to treatment with the proposed drug, all patients received standard comprehensive treatment. Against the background of conventional allopathic therapy, patients were prescribed taking the proposed homeopathic remedy in the appropriate dosage for 1 month. Before the start of taking the drug and after the end of the course of treatment, the content of DC and MDA in the blood plasma was determined. Prior to taking the proposed drug, there was an increase in the content of MDA in patients of all groups (by 93.3%, 108.2% and 134.1%, respectively) and DC by 67.8%, 79.4% and 99.2%, respectively compared to control. One month after administration, favorable LPO dynamics was noted: in patients of the 1st group, the content of MDA decreased by 37.5%, DC by 19.9%, in patients of the 2nd group MDA by 41.8% and DC by 21.4%, In the 3rd group, the content of MDA decreased by 50.7%, and the content of DC by 33.8%.

 Changes in the biochemical parameters of lipid peroxidation correlated with a clinical improvement in the condition of patients, which allowed a significant reduction in the dose of allopathic drugs, and in some patients it was completely canceled (for example, in patients with stable angina pectoris I-II functional class).

 Thus, the homeopathic Rejuvenating Agent has a pronounced therapeutic effect in diseases accompanied by an increased content of free radicals and activation of lipid peroxidation of cell membranes. The tool can be used both monotherapy and in the complex treatment of diseases. In addition, the proposed drug can be used as a prophylactic to reduce the harmful effects of unfavorable environmental factors on the body, to slow down the aging process.

 The tool has no side effects, contraindications, does not give allergic reactions, can be used in general clinical and geriatric practice.

Claims (2)

 1. Homeopathic antioxidant product, characterized in that it contains Ferrum iodatum, Carbo vegetabilis, Lachesis, taken in equal proportions and when diluted C6.  2. Homeopathic remedy according to claim 1, characterized in that it is made in the form of sugar granules.