RU2173159C2 - Method of production of biologically active food addition - Google Patents

Abstract

FIELD: biotechnology, animal husbandry. SUBSTANCE: method involves culturing microorganisms in nutrient medium, separation of bacterial mass from cultural medium, its mixing with protective medium and drying. Microflora composition has bifidobacterium of two species, namely, acidophilic bacillus and propionic acid bacterium. For this purpose fermentation is carried out with pure cultures of Bifidobacterium longum T- 89, Bifidobacterium thermophilum TP-8, Lactobacillus acidophilus 97 and Propionibacterium shermanii P- 1 which are added in the amount 8-10% of medium volume and ratio of cultures is corrected to 2.5:2.5:1:4, respectively. EFFECT: significantly enhanced curative-prophylactic properties of biologically active addition as result of simultaneous effect of microorganism cultures used. _

Description

 The invention relates to biotechnology and can be used in animal husbandry.

 In the context of industrial livestock farming, the prevention of gastrointestinal diseases of young animals caused by opportunistic and pathogenic microflora should be aimed at increasing the level of natural resistance of animals. This can be achieved by accelerating the creation of a normal intestinal biocenosis using biologically active agents containing symbiotic intestinal microflora as bifidobacteria. It is advisable to introduce bifidobacteria as natural factors of nonspecific protection of a macroorganism into young diets during the entire growth period: to accelerate the colonization of intestinal bifidobacteria immediately after birth, which helps protect newborns from opportunistic and pathogenic microorganisms, as well as restore and maintain the necessary level of bifidobacteria in case of dysbacteriosis arising from stressful situations and treatment with antibiotics and antibacterial drugs, in order to replace antibiotics Otik in animal nutrition.

 To enhance the therapeutic and prophylactic properties of bifidobacteria, it is advisable to use bifidobacteria with other representatives of normal intestinal microflora.

 Known bacterial preparations "bifidumbacterin", "lactoamilovorin", "bifacidobacterin", "bifimol", "probiovet" and others, as well as biologically active feed additives and agents / 1,2,3 /, which have therapeutic and prophylactic properties, are composed of microflora which are administered bifidobacteria. The technology for producing bacterial preparations and biologically active feed additives includes the preliminary cultivation of microorganisms in various nutrient media in order to accumulate biomass, the concentration of biomass, as well as its conservation, which in most cases involves the use of drying in various ways.

The closest in technical essence to the invention is a method for producing a bacterial preparation for a feed containing bifidobacteria and lactic acid bacteria, which provides for the accumulation of biomass of bifidobacteria together with acidophilus bacillus and lactic streptococci (Pat. 2035184 Russia, MKI ⁶ A 61 K 35/66, C 12 N 1/20) / 4 /.

 The disadvantage of this method is the use in the composition of the starter microflora only cultures of bifidobacteria (of the same species) and lactic acid bacteria, which reduces the preventive properties of the drug.

 The aim of the invention is to increase the therapeutic and prophylactic effectiveness of biologically active feed additives.

 The essence of the invention lies in the integrated use of microflora biologically active feed additives of bifidobacteria, acidophilus bacillus and propionic acid bacteria. For this purpose, it is proposed to carry out the fermentation of the nutrient medium with the following cultures: Bifidobacterium longum T-89, Bifidobacterium thermophilum TP-8, Lactobacillus acidophilus 97 and Propionibacterium shermanii P-1.

The effectiveness of a biologically active feed additive obtained by the proposed method is determined by the simultaneous action of all the crops used. An additional introduction to the microflora of a biologically active additive obtained by our technology, propionic acid bacteria, which are active producers of B vitamins (B ₁₂ , B ₂ , pantothenic and nicotinic acids), can increase the therapeutic and prophylactic properties of the additive in comparison with the known probiotic drugs.

Example. When implementing the proposed method, bacterial strains are used, which are the property of the Museum of Pure Microorganism Cultures of the Department of Epizootology and Microbiology of VGMHA named after N.V. Vereshchagin and maintained in working condition. Bacteria are grown on a nutrient medium of the following composition: lactose - 5%, peptone - 5%, milk whey - 10%, yeast extract - 5%, corn extract - 1%, iron sulfate 7 - water - 0.5%, hydrolyzed milk - 10%, water - up to 100%, pH 6.6. The nutrient medium is sterilized at 121 ^o C for 15 minutes, then cooled to 37 ^o C and fermented with pure cultures of Bifidobacterium longum T-89, Bifidobacterium thermophilum TP-8, Lactobacillus acidophilus 97 and Propionibacterium shermanii P-1 with an initial culture ratio of 2.5 : 2.5: 1: 4, which contribute in the amount of 8-10% by volume of the medium. Co-cultivation of microorganisms is carried out at 37 ^o C for 20-24 hours to achieve a pH of 4.6. At the end of growth, the culture medium is cooled to 14 ^° C. and the biomass is separated from the culture medium by centrifugation. The resulting biomass is mixed with a protective medium and dried.

The proposed method for producing biologically active feed additives will allow:
to carry out the integrated use of cultures of bifidobacteria, acidophilus bacillus and propionic acid bacteria;
get in 1 g of the additive the content of viable cells of bifidobacteria up to 10 ¹² , acidophilus bacillus - up to 10 ⁹ , propionic acid bacteria - up to 10 ⁹ ;
use the additive directly for the prevention of gastrointestinal diseases of young farm animals or use it for the enrichment of feeds based on dairy raw materials.

Sources of information
1. Platonov A. V. Production of livestock products based on microorganisms-symbionts of the gastrointestinal tract. -M .: 1985, -44 p.

 2. Grunskaya V.A., Ervolder T.M., Gudkov S.A. The use of bifidobacteria in the diets of young farm animals. -M .: AgroNIITEIMP. -1991. -24 p.

 3. TU 10. 02.925-91. Substitute of whole milk, dry fermented milk "3TSM-U" for calves.

Claims (1)

 A method of obtaining a biologically active feed additive, comprising preparing a nutrient medium, introducing the starter culture of bifidobacteria and acidophilus bacillus, accumulating the biomass of microorganisms and separating it from the culture medium, mixing the biomass with a protective medium and drying, characterized in that, in order to improve the therapeutic and prophylactic properties, a culture of propionic acid bacteria is additionally introduced into the starter culture, the medium is fermented with pure cultures of Bifidobacterium longum T-89, Bifidobacterium thermophilum TP-8, Lactobacillus acido philus 97 and Propionibacterium shermanii P-1 with an initial crop ratio of 2.5: 2.5: 1: 4, which contribute 8-10% to the volume of the medium and are grown together at a temperature of 37-38 ° C for 20-24 hours to achieve a pH of 4.6.