RU2161955C1 - Method of varying physico-chemical and biological properties of substance - Google Patents

Abstract

FIELD: material engineering. SUBSTANCE: in order to change physico-chemical and biological properties of a substance, to the latter, potentialized form, prepared by consecutive progressing dilutions of initial substance and repeated vertical shaking or triturating according to homeopathic dynamization technique, is added. EFFECT: simplified and secured procedure. 2 cl, 6 ex

Description

 The invention relates to processes aimed at changing the properties of a substance, and can be used in various fields of science and technology, mainly in physics, chemistry, biology, medicine, food industry, etc.

The prior art it is known to use external physical effects on a substance to change its physico-chemical and / or biological properties. As an external physical effect, in particular, heating is used (see RF patent 2075127, class A 62 D 3/00, G 21 I 3/00, 1997), which reduces the toxicity of chemicals due to thermal decomposition; treatment with an electric and magnetic field, which changes the physical properties of radioactive materials (see RF patent 2061266, CL G 21 F 9/00, 1996), metals (see RF patent 2042737, CL C 22 F 3/00, 1995), water (see ed. St. USSR 419212, class A 01 N 59/04, 1976; RF patent 2032424, class A 61 K 41/00, 1995), an alcohol-containing mixture ( see RF patent 1825374, class C 21 G 3/06, 1993), increases the production of antibodies during immunization with an antigen (see ed. St. USSR 1178450, class A 61 K 39/00, 1985) ; ultraviolet radiation, which initiates various polymerization reactions (see ed. St. USSR 146039, class C 08 F 2/48, 1962), increases the protective, therapeutic properties of drugs (see RF patent 2034550, class A 61 K 35/12, 1995), or ionizing radiation, which increases the biological activity of minerals (see RF patent 2030910, class A 61 H 39/00, 1995), provides inactivation of tumor cells during processing of the implant, ensuring maximum preservation its native properties, initiates the course of various polymerization reactions (see ed. St. USSR 183389, class C 08 F 2/46, 1966)
However, these known methods for changing the physicochemical and / or biological properties of a substance have a common drawback, which lies in the technological complexity and the possibility of a harmful effect on the human body (personnel serving the equipment).

 The invention is aimed at creating a technologically simple, safe and effective way to change the physicochemical or physicochemical and biological properties of a substance.

 The solution to this problem is provided by the fact that in the method of changing the physicochemical or physicochemical and biological properties of a substance by physical action, according to the invention, the latter is carried out by introducing into the starting substance its potentiated form obtained by successive progressive dilution and multiple vertical shaking or grinding of the original substances according to the homeopathic method of dynamization.

In this case, the potentiated form of the substance is introduced in a hundred-percent dilution of 1: 100 ¹⁰ - 1: 100 ¹⁰⁰⁰ in an amount of 0.1 - 0.0001 wt.% Of the starting material.

 The claimed method is simple and technologically advanced and safe for staff.

 The potentiated form of the starting material is prepared according to a well-known homeopathic technique (see V. Schwabe "Homeopathic Medicines", M., 1967, p. 14-29) by repeated serial dilution or grinding and external exposure as follows.

The starting material is dissolved in a neutral solvent (distilled water, alcohol, physiological saline, etc.), producing a uniform progressive decrease in concentration by successive dilution of 1 part of the starting material in 9 parts (for ten-fold dilution) or in 99 parts (for hundred-percent dilution) , or in 999 parts (for the thousandth dilution) of a neutral solvent with multiple vertical shaking (dynamization) of each dilution obtained and using separate containers for each the next dilution to obtain a given ratio, mainly hundred dilutions 1: 100 ¹⁰ - 1: 100 ¹⁰⁰⁰ (C 10 - C 1000).

 The resulting potentiated form is introduced into the starting material in an amount of 0.1 - 0.0001 wt.%.

 To obtain a potentiated form of a solid substance which is poorly soluble in a neutral carrier, the latter is triturated to obtain a powder, one part of which is triturated for 99 hours with 99 parts (to obtain a hundred-percent dilution) of lactose (neutral carrier). Then one part of the resulting mixture is similarly triturated with 99 parts of lactose, etc. until the given multiplicity is obtained.

The resulting potentiated form is mixed with the starting material in the form of a powder or in a molten (liquid) state
Example 1

 In studying the effect of its potentiated form introduced into the substance on the change in physicochemical properties, we studied the kinetics of the process of the fundamental hydrolysis of adenosine triphosphate (ATP) in the physiological pH range with citrate buffer by nuclear magnetic resonance (NMR) method. In the table. Figure 1 shows the results of studies of the rate of hydrolysis reaction when making: a) a potentiated form of ATP and b) a potentiated form of a buffer in the reacting components.

 Given in the table. 1, the experimental results showed a slowdown in the rate of hydrolysis reaction if its potentiated form is additionally introduced into one of the components.

 Example 2

 In the table. 2 shows the results of studies of changes in the toxicity of a substance when making its potentiated form. The toxic substance morphine and the toxic substance morphine, together with its potentiated form in a dilution of C1000 in the amount of 0.1 wt.% Were administered to experimental animals - mice.

для самцов:

В числителе указаны данные для животных, получивших морфин, а в знаменателе - данные для животных, получивших морфин в сочетании с его потенцированной формой.In the numerator is the control number of animals that received morphine, in the denominator is morphine in combination with its potentiated form. As a result of the experiment, it was found that the lethal dose of LD (mg / kg) is
for females:

for males:

The numerator shows data for animals that received morphine, and the denominator shows data for animals that received morphine in combination with its potentiated form.

 From the results of the experiment it follows that when introduced into a toxic substance - morphine of its potentiated form, a change in toxicity occurs.

 Example 3

In the table. Figures 3 and 4 show the results of changes in the metabolic activity of a substance when introduced into the latter of its potentiated form. During the experiment, 50 experimental animals (rats) were injected once or chronically (within 14 days) with 1.5 ml: 1) 45% ethanol; 2) 45% ethanol, in which 0.01 ml of potentiated ethanol was added in a dilution of C30 (concentration of ^10-60 wt. Hours); 3) 45% ethanol, in which 0.1 ml of potentiated ethanol was introduced in a dilution of C200 (concentration of 10 ^-400 parts by weight) and after cervical dislocation in animals, the content of dopamine, norepinephrine, adrenaline and serotonin in the brain and blood was determined by the separation of these substances for carboxymethyl cellulose followed by spectrofluorimetric detection.

 From the table. 3 and 4 it follows that the introduction of its potentiated form into the starting material leads to a significantly different change in metabolic activity.

 Example 4

A change in the biological activity of a substance (physicochemical and biological properties) when a potentiated form is introduced into it was experimentally established when studying dynamics of post-genetic potency (DPTP) on surviving hippocampal sections of animals, the electrophysiological phenomenon of which is that in serial irritation by electric current through a stimulating electrode located in the zone of mossy fibers, in the zone of the SAZ of the hippocampus, a long-lasting total the exciting postsynaptic potential. In a series of experiments, a classic example for neurobiology was reproduced, which consists in the complete suppression of DPTP by a native monospecific antiserum to the brain-specific protein S-100. As a result of the experiments, it was found that the application of hippocampal slices with a mixture containing 10 ml of native antiserum to the brain-specific protein S-100 and 1 ml of potentiated alcohol solution prepared from it in a dilution of C6 (concentration of 10 ^-12 parts by weight) is accompanied by the cancellation of the "classical the "blocking effect of the native antiserum, i.e. formation of crashes is observed.

 Example 5

 0.1 g of the starting morphine hydrochloride powder was triturated according to a technique known in homeopathy for an hour with 9.9 g of lactose (a neutral carrier). Then, 1 g of the resulting mixture was similarly triturated with 99 g of lactose and 1 g of this mixture was again triturated with 99 g of lactose, thereby obtaining 3 hundred rubbing of morphine hydrochloride. Then, 0.1 g of 3-centimeter rubbing was mixed with 10 g of the initial morphine hydrochloride powder and the mixture was dissolved in distilled water to a 1% solution.

 The solution obtained according to the described method was administered intraperitoneally with a daily dose increase from 1 to 10 mg / kg (animal weight) for 10 days to the experimental group of rats. In the control group, rats were injected with a 1% morphine solution for 10 days, also increasing the dose from 1 to 10 mg / kg. Irritating the limbs of animals in both groups with electric current, we determined the thresholds for the nociceptive reaction of rats. Analysis of the obtained data showed that in the experimental group the thresholds of pain sensitivity were significantly higher than in the control, which indicates an increase in the analgesic effect of morphine hydrochloride under the influence of its potentiated form.

 Example 6

Antibodies to morphine (antiserum) were prepared according to a well-known technique (see, for example, the book. Immunological methods, under the editorship of G. Fremel, M .: Medicine, 1987, p. 9-33). Then, from the obtained antiserum according to the method described above, a potentiated form in dilution of C50 was prepared by successively reducing the concentration, 1 ml of which was introduced into 10 ml of the previously obtained antiserum to morphine. The resulting mixture was incubated for an hour in a thermostat at a temperature of 37 ^o C. Next, all stages of an enzyme-linked immunosorbent assay (ELISA) were carried out: washing, detection of the formed immune complexes using anti-virus antibodies labeled with an enzyme, and spectrophotometric recording of the results at 492 nm. At the same time, the effect of a decrease in the optical density in ELISA by 40% was observed.

Claims (2)

 1. A method of changing the physicochemical or physicochemical and biological properties of a substance by physical action, characterized in that the physical effect is carried out by introducing into the starting material its potentiated form obtained by successive progressive dilution and repeated vertical shaking or grinding of the starting material according to the homeopathic method dynamization. 2. The method according to claim 1, characterized in that the potentiated form of the substance is introduced in a hundred-percent dilution of 1: 100 ¹⁰ - 1: 100 ¹⁰⁰⁰ in an amount of 10.0 - 0.0001 wt.% Of the starting material.

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