RU214244U1 - DEVICE FOR ISOstatic PRESSING - Google Patents

Abstract

The utility model relates to devices designed to act on the treated object with high isostatic pressure, in particular on the dermis in order to decellularize it (suppress the viability of dermal cells while maintaining collagen structures). The essence of the utility model lies in the fact that in a device for isostatic pressing, which includes a coaxially located pressing chamber designed to fill it with a compressible medium and to place a processed object in it, as well as a press piston installed in the pressing chamber with the possibility of axial movement, according to useful model, the press piston on the end side facing the compressible medium in the working position has a blind recess, the cross-sectional area of \u200b\u200bwhich is commensurate with the cross-sectional area of the object being processed. The technical result achieved in the implementation of the utility model is the intensification of the process of decellularization of the dermis. 3 ill.

Description

The utility model relates to devices designed to influence the treated object with high isostatic pressure, in particular, the dermis in order to decellularize it (suppress the viability of dermal cells while maintaining collagen structures).

Devices for isostatic pressing are known, including a compression chamber filled with a compressible medium (liquid, gas), in which the object to be processed is placed, and a press piston that exerts pressure on the compressible medium (see, for example, Bulletin of PNRPU Mechanics, No. 2, 2013. And .M. Berezin "Creation of an experimental setup for hydrostatic compression of porous materials").

Known devices for isostatic pressing, used to influence biological objects in order to process them and modify their properties.

For example, a device for isostatic pressing [JPH 02171172 (A)] is known for pressurizing a food product, in particular tuna.

The device under consideration contains a coaxially located pressing chamber filled with a compressible medium, in particular, a liquid, designed to accommodate a processed object in it, and a press piston installed in the pressing chamber with the possibility of axial movement, exerting a pressing effect on the liquid.

As a result of this effect on the product placed in the pressing chamber, its sterilization is achieved.

A known device for isostatic pressing [EN 198359], selected as the closest analogue (prototype).

This device is designed to influence the treated object, in particular, the dermis, with high isostatic pressure.

The device under consideration contains a coaxially located chamber designed to fill it with a hydraulic medium and to place a processed object in it, as well as a piston installed in the pressing chamber with the possibility of longitudinal movement, connected with a means for creating a high hydrostatic pressure.

As a result of the treatment of the dermis in the considered device with high pressure, its decellularization is achieved.

However, this device does not provide for the presence of constructive means that contribute to the intensification of the process of decellularization of the dermis, carried out under the influence of high isostatic pressure.

The technical problem to be solved in the implementation of the utility model is the intensification of the process of decellularization of the dermis.

The essence of the utility model lies in the fact that in a device for isostatic pressing, which includes a coaxially located pressing chamber designed to fill it with a compressible medium and to place a processed object in it, as well as a press piston installed in the pressing chamber with the possibility of axial movement, according to useful model, the press piston on the end side, facing the compressible medium in the working position, has a blind recess, the cross-sectional area of \u200b\u200bwhich is commensurate with the cross-sectional area of the object being processed.

The presence in the claimed device of the compression chamber and the press piston, through which the pressure is transferred to the compressible medium filling the compression chamber, makes it possible to treat the object (dermis sample) with high isostatic pressure in order to decellularize it.

As the authors' studies have shown, the process of decellularization of the dermis is intensified when its cells are saturated with compressed gas in the process of exposing it to high pressure.

The process of diffusion of compressed gas into the cells of the dermis can be carried out in the inventive device when treating the dermis with high isostatic pressure due to the pressing effect of the press piston on the two-phase compressible medium filling the pressing chamber, including the bottom layer of liquid, in which the processed dermis sample is located, and located above the layer liquid gas.

At the same time, it is important that the inventive device has a recess made in the press piston, which plays a significant role in intensifying the process of decellularization of the dermis sample placed in the compression chamber in the liquid layer under the specified recess.

When the pressing chamber is filled with said two-phase medium, during the movement of the press piston in the gaseous medium, the gas under the action of the pressing force is compressed and dissolved in the bottom layer of the liquid, saturating it. The compressed gas reaching the dermis sample diffuses into the liquid of the dermis cells, which contributes to the intensification of the process of its decellularization.

When the pressure piston reaches the liquid level, the gas that has not dissolved in the liquid is localized in the recess.

With further movement of the press piston, the liquid begins to fill the recess, the pressure and the rate of gas dissolution in the liquid in the recess increase, and in the limiting case, when the recess is completely filled with liquid, all the gas dissolves in the liquid. In this case, the concentration of compressed gas in the liquid volume localized above the dermis sample turns out to be extremely high.

Since the cross-sectional area of the notch is commensurate with the cross-sectional area of the treated dermis sample, the entire dermis sample is exposed to a liquid with a very high concentration of pressurized gas in it.

Due to this, the process of saturation of the intracellular fluid of the dermis sample with compressed gas is actively going on, which contributes to the intensification of the process of its decellularization.

The advantage is the location of the recess on the end side of the press piston in its central part, which is technologically advanced and simplifies the calculation of the parameters of physical quantities that affect the process of processing the dermis.

Thus, the technical result achieved in the implementation of the utility model is the intensification of the process of decellularization of the dermis.

In FIG. 1 shows a general view of the device when moving the press piston in a gaseous medium; in fig. 2 - the same when the press piston reaches the liquid level; in fig. 3 - the same when filling the recess with liquid.

The device contains coaxially located pressing chamber 1 and press piston 2. Pressing chamber 1 is intended for filling it with a compressible medium and for placing a processed object 3 (derma sample) in it. The press piston 2 is installed with the possibility of axial longitudinal movement in the pressing chamber 1. The press piston 2 is connected to a means (not shown in the drawing) of creating pressure. By means of the press piston 2, the pressure generated by the load applied to the press piston 2 is transferred to the compressible medium filling the press chamber 1.

The press piston 2 on the end side facing the compressible medium in the working position has a central blind recess 4, the cross-sectional area of which is commensurate with the cross-sectional area of the processed object 3.

The device works as follows.

The pressing chamber 1 is filled with a medium, including the bottom layer of liquid 5 and gas 6 occupying the remaining volume of the pressing chamber 1.

A load is applied to the press piston 2, under the action of which it carries out axial longitudinal movement in the pressing chamber 1 and exerts a pressing effect on the medium located in the pressing chamber 1.

During the course of the press piston 2 from the start of movement to contact with liquid 5 (Fig. 1), gas 6 dissolves in liquid 5 over its entire surface (the dissolution process in the drawing is schematically shown by arrows.

Compressed gas reaching the cells of the dermis diffuses into the liquid of the cells of the dermis.

When the press piston 2 comes into contact with liquid 5 (Fig. 2), the dissolution of gas 6 in liquid 5 over its entire surface stops, and the remaining gas is localized in recess 4.

With further movement of the press piston 2 (Fig. 3), the gas 6 is compressed in the recess 4. The pressure and the rate of dissolution of the gas 6 in the liquid 5 increase significantly. In the limiting case, when the entire recess 4 is filled with liquid 5, the concentration of compressed gas 6 in the volume of liquid 5, localized above the object 3 being processed, is extremely high, which causes its intense effect on the dermis sample.

Thanks to the described process, an intensification of the decellularization of the dermis is achieved.

Claims (1)

A device for isostatic pressing, which includes a coaxially located pressing chamber designed to fill it with a compressible medium and to place an object being processed in it, as well as a press piston installed in the pressing chamber with the possibility of axial movement, characterized in that the press piston on the end side, facing the compressible medium in the working position, has a blind recess, the cross-sectional area of which is commensurate with the cross-sectional area of the object being processed.

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