RU2131252C1 - Method of preparing inulin from jerusalem artichoke tubers for medicinal and food aims (variants) - Google Patents

Abstract

FIELD: biotechnology, natural compounds. SUBSTANCE: method involves preparing sap and an aqueous extract from fresh tubers and husks or dried milled tubers. The latter are obtained by mixing the dried milled tubers with hot water at ratio 1:6, kept at 70 C for 12 h and extraction is carried out at 80 C. An aqueous extract is separated, extracted three times ageing with water at ratio 1:4 for 40 min followed by extracts combining. Purification of sap diluted with water (1:1) obtained from husk aqueous extractions or dried tubers is carried out with calcium carbonate at 85 C or 80 C for 1 h for sap and husks and dried tubers, respectively. Extracts are filtered, filtrates are evaporated and crystallization of crude inulin is carried out at 4 C into cooling for 5 days from aqueous extracts after alcohol addition at ratio 1:1 or from the diluted sap. Inulin precipitates are separated and dissolved in hot water at 75 C. Additional purification involves stages using anionite, aluminium oxide, calcium carbonate followed by purification using cationite and anionite. Inulin is crystallized and reprecipitated from an aqueous-alcohol mixture (1:3) at 4 C for 24 h into cooling. Obtained product is dried and milled. EFFECT: simplified technology of inulin purification, standardized the end product. 4 cl, 2 ex

Description

 The invention relates to the pharmaceutical industry, relates to a method for the production of inulin from Jerusalem artichoke tubers (Helianthus tuberosus L.), which is an agricultural crop.

It is known that inulin is a fructose polysaccharide (polyfructosan), is a product of photosynthesis of some plants and is a mixture of structurally similar polymer analogues containing about 35 fructose fragments, with a variable specific rotation [α] _D from -32 to -40 ^o . The high molecular weight inulin polymer, which is isolated by recrystallization from water, can have [α] _D = -40 ^o / 1, 2, 3, 4 /.

 Inulin is a good dietary product and therapeutic product for patients with diabetes, because assimilation of fructose to a much lesser extent depends on the hormone of the pancreas of insulin than for glucose. Moderate consumption of fructose does not cause pronounced changes in blood sugar levels / 5 /.

Inulin, as a medical product, and its dosage form for injection, is part of the American Pharmacopoeia. According to Official Monographs USP XXII article "Inulin", the content of C ₆ H ₁₁ O ₅ (C ₆ H ₁₁ O ₅ ) OH in inulin is not less than 94% and not more than 102%, calculated on the dry preparation / 3 /.

 Inulin is also used to obtain a number of derivatives for medical use / 6, 7, 8, 9 /.

 Known methods for producing inulin from various plant sources: from dahlia tubers (Methods of carbohydrate chemistry. / Transl. From English. Ed. By N.K. Kochetkov. Ed. Mir, 1967. - S. 370 - 371. / 2 /; Lazurevsky G.V. et al. Practical Work on the Chemistry of Natural Compounds, Higher School Publishing House, Moscow - 1966. - P. 68 - 69. / 10 /); from the roots and rhizomes of plants of the genus Elecampane (Auth. Witness. USSR, M. cl. 4 A 61 K 35/78, 3785968 / 28-14, 08.20.94, VNIIKhTLS, Zinchenko V.V. et al. Method for producing inulin / 11 /; Auth. Certificate of the USSR, Particleboard, AG 1709612 A 1, M. cl. A 61 K 35/78, 1984, 4808959/14, 03/30/90, Institute of Chemical Sciences, Kyrgyz SSR, Turdumambetov K. and others. A method of obtaining inulin / 12 /); from algae (B.J. D. Mecuse, Inulin in the green alga Batophora oerstedi J. Ag, (Dasycladales). "Acta Botanica Neerlandica", 1963, 12, 315 - 318. / 13 /); from inulin-containing raw materials (Aut. witness of the USSR, class C 1313/00, N 487118, application 05.03.73 N 1891936, publ. 02.26.76. Fedotkin IM and others / Kiev Technological Institute of Food . prom-ty /. A method of producing inulin. / 14 /.

 A number of methods for producing inulin from Jerusalem artichoke tubers have been described (Mino Joshiki, Tsutsui Satomi, Ota Nagayo. Separation of Acetylated inulin by reversed-phase. "Chem. And Pharm. Bull", 1985, 33, N 8.3503-3506. / 4 / ; Golubev V.R. et al. Jerusalem artichoke. Composition, properties, processing methods, applications. M. - 1995. - S. 60 - 68. / 15 /; RF Patent, 20011621, 5 A 61 K 35/78, 31/715, C 13 K 11/00. 5044672/14, 05/29/92, Borisenko V.G. et al. Method for producing inulin. / 16 /), however, none of them give the output of inulin from its content in raw materials and the degree of purity of the obtained product from related substances. Therefore, such inulin can be considered a technical or raw product, not suitable for use in medicine.

 We found that crude inulin obtained by precipitation with alcohol or after a single crystallization contains impurities such as pectin, protein and amino acid residues, organic acids, phenolic compounds and their oxidation products. Impurities are detected by paper chromatography with appropriate diagnostic reagents or in the UV light of a fluorescent lamp.

We can note the disadvantages of these methods / 4, 15, 16 /. The use of lead acetate for the deposition of colloids is not medically justified due to the toxicity of the metal ion. The use of ultrafiltration for the purification of inulin requires special filters and membranes, which are not widely available for industrial use. In addition, the purification of extracts from Jerusalem artichoke using ultrafiltration and filtration on membranes does not make it possible to free extracts containing inulin from high molecular weight natural polymers of proteins and pectins. Extraction with 25% alcohol will lead to a partial loss of inulin, which is poorly soluble in alcohol solutions and will not be fully extracted under these conditions. Acidification of an inulin solution can lead to loss of inulin. For example, 0.2 N. a solution of sulfuric acid causes hydrolysis of inulin for 10 to 15 minutes at 70 ^o C / 17 /.

 For the prototype method for producing inulin from Jerusalem artichoke tubers, you can take the method proposed by Borisenko V.G. with co-authors / 16 / ".

 The technical result of the invention is to create a unified method for producing the final product - inulin using raw materials from Jerusalem artichoke with an extension of its processing time and to develop a more affordable purification of inulin upon receipt.

To achieve this technical result, the proposed method for producing inulin provides for obtaining juice from raw materials and extracting extractives from squeezed and from raw materials with hot water at 80 ^o C for 60 minutes with preliminary and subsequent purification of inulin with sorbents in aqueous solutions, evaporation of the solution and crystallization of inulin from aqueous and aqueous-alcoholic solutions.

 As a raw material, crushed Jerusalem artichoke tubers, as well as tuber powder, are used. A mixture of powder and water was incubated for 12 hours to swell. Shredded raw tubers are used immediately.

 A method of producing inulin is as follows. As a raw material at the beginning of the season, chopped raw Jerusalem artichoke tubers are taken, and then powder from Jerusalem artichoke tubers is used. This is due to the fact that raw tubers are poorly stored, and with prolonged storage, inulin in tubers is gradually subjected to enzymatic hydrolysis with an increase in glucose (Furmanova I. B., Kalunyants K. A., Krikunova L. N. Biochemical change in Jerusalem artichoke during storage // Ed. J. Izv.Vyssh. Food Technology. - Krasnodar, 1989 .-- 10 p.).

 Subsequent use of Jerusalem artichoke tubers powder as a raw material for inulin production allows the enterprise to work smoothly for a long time. The literature describes a method for producing a powder (Tertychnaya T.N., Korneeva O.S., Zherebtsov N.A. A method for producing powdered semi-finished products from Jerusalem artichoke. // Problems of resource-saving and environmental technologies and equipment for processing and storage of agricultural raw materials: Abstract. Ross. Scientific and practical conference with international participation. - Krasnodar. - 1993. - P. 15.) The enterprise itself can produce powder in any quantity.

 The output of inulin should be at least 75% of its content in Jerusalem artichoke tubers, which varies from 2 to 18% according to the literature / 18, 19 / depending on the collection time and storage conditions of tubers. 7.7% inulin based on dry tubers was found in this raw material.

The juice is squeezed using a juicer. Water extracts are additionally obtained from squeezes, similarly, aqueous extracts are obtained from dry raw materials (dried tubers), which are pre-filled with hot water and allowed to swell for 12 hours. The extracts are carried out by heating for 60 minutes at 80 ^° C, filtered and squeezed out in hot view through a layer of calico.

Juice diluted with hot water (95 ^o C) 1: 1, and separately, hot aqueous extracts are treated with calcium carbonate (chalk) at 80 - 85 ^o C for 60 min and filtered hot through a layer of calico. This makes it possible to destroy the inulin-pectin complex, coagulate proteins, free from water-soluble pectins, partially from organic acids, proteins, without destroying inulin, where the pH remains close to neutral. Filter cake in all cases, washed with hot water (85 ^o C) to obtain the volume of the taken stock solution.

The filtrate of the aqueous juice solution is evaporated under vacuum or on an evaporation cup at 75 ^° C to 250-300 ml, the filtrates of aqueous extracts from pomace and aqueous extracts from dry tubers are also evaporated to 300-400 ml.

Crystallization of inulin is carried out at 4 ^o C in the refrigerator for 5 days. One stripped off aqueous solution of juice put on crystallization without the addition of alcohol. To evaporated aqueous extracts add alcohol 1: 1. The precipitation of raw inulin is gray.

The precipitates of crude inulin are separated by fitting and combined while still wet, and dissolved immediately in water at 75 ^° C. The hot inulin solution is filtered through cotton cloth from possible mechanical impurities and the filtrate is further purified.

For this, the filtrate, cooled to 45 ^o C, is passed through a column with anion exchange resin in the OH ^- form. The column is washed with water at 45 ^o C to a volume equal to the taken initial solution. Anion exchange resin allows you to remove organic acids, phenolic acids and other compounds of an acidic nature, as well as anions of organic and inorganic salts; aluminum oxide is added to the eluate and heated with constant stirring for 30 minutes at 75 ^° C, filtered through a layer of calico and the filter cake is washed with hot water. This allows you to remove impurities adsorbed on alumina (phenols, polyphenols, their oxidation products); calcium carbonate (chalk) is added to the obtained filtrate, heated at 75 ^° C for 30 minutes with constant stirring, and the carbonate is separated by filtration through a layer of calico, which allows you to bind soluble pectins in the form of insoluble calcium salts and a significant part of the aluminum ions that enter the solution; the hot filtrate (75 ^° C.) was again vacuum filtered through an alumina layer interposed between paper filters and pre-washed with hot water. Such filtration makes it possible to remove mechanical particles of aluminum oxide and chalk; the filtrate is cooled to 45 ^o C and sequentially passed through a cation exchanger in the H ⁺ form and immediately through an anion exchanger in the OH ^- form with washing the columns with water at 45 ^o C, which allows you to free both from cations and from anions of inorganic and organic origin and at the same time neutralize hydrogen ions after cation exchange resin. Get a colorless or yellowish opalescent solution of the eluate with a pH of 6.5 to 7.3; in the presence of a yellow color of the solution, it is decolorized with activated carbon when heated for 30 minutes at 60 ^° C, followed by separation of coal from the hot solution through a dense filter. To the resulting solution was added 96% alcohol 1: 3 and inulin was crystallized in the refrigerator at 4 ^o C for 1 day; the precipitated inulin precipitate is reprecipitated twice from the minimum volume of the aqueous solution with 96% alcohol (1: 3) and crystallized in the same way; the precipitate of inulin after reprecipitation is filtered on a paper filter, washed twice on the filter with 85% alcohol, transferred to 96% alcohol, shaken for 30 minutes and filtered; the precipitate is dried in air on the substrate of their food film and crushed in a mortar. A white powder is obtained with a moisture content of 10% and a specific rotation of [α] _D = -36.5 ^o .

 The purity of inulin is controlled by chromatography on paper in the solvent system butanol - acetic acid - water 4: 1: 2.

The resulting inulin should contain no impurities of reducing carbohydrates (aldoghexoses), amino acids, phenolic acids, groups of phenols and polyphenols when processing the chromatogram with diagnostic reagents. On the starting line, inulin (polyfructosan) is detected with urea. Free fructose is not detected. After hydrolysis of inulin 0.2 N. sulfuric acid for 30 min at 80 ^o C, only fructose and traces of glucose are detected chromatographically. The latter is an integral part of the polymer molecules of inulin in a very small amount.

Auxiliary materials and sorbents are regenerated as necessary after work: calcium carbonate is calcined at 800 ^o C in a muffle furnace, the resulting quicklime is quenched with water and air is bubbled through the mixture. The resulting suspension of calcium carbonate is defended, separated, dried and used in subsequent work; aluminum oxide is calcined at 800 ^o C in a muffle furnace, cooled and used in subsequent work; cation exchanger and anion exchanger regenerate by passing through the columns, respectively, 1 N. hydrochloric acid solution and 1 N. sodium hydroxide solution followed by washing the ion exchangers with distilled water to a neutral reaction according to the indicators: cation exchange resin according to methyl orange, anion exchange resin according to phenolphthalein or control is carried out using a universal indicator paper. Get cation exchange resin in the H ⁺ form, anion exchange resin in the OH ^- form and use in further work; alcohol was regenerated by distillation.

 Example 1. Of 1000 g of raw chopped Jerusalem artichoke tubers (with a moisture content of 75.7%) squeeze 500 ml of juice on a household juicer (pH 6.38).

500 ml of water heated to boiling (1: 1) is added to the juice, heated to 80 ^° C, 50 g of calcium carbonate (chalk) are added and heated with constant stirring for 60 minutes at 85 ^° C on an electric stove with an asbestos mesh. The hot mixture is filtered through a layer of calico. The filter is washed with 150 ml of hot water (85 ^o C). A filtrate volume of 1000 ml was obtained (pH 7.02), which was evaporated on an evaporation cup in a water bath at 75 ^° C. or under vacuum to a volume of 250 ml (pH 6.98). One stripped off solution is placed on crystallization in a refrigerator at 3-4 ^o C for 5 days. A gray precipitate of crude (untreated) inulin will precipitate.

Wet squeezes remaining after juice separation (about 450 g) are extracted twice with water in 1.5 L portions at 80 ^° C for 60 minutes and squeezed hot through calico. Get 3 l of aqueous extraction from pomace (pH 6.6).

45 g of calcium carbonate was added to the hot aqueous extract and heated at 85 ^° C. with constant stirring for 45 minutes. The hot mixture is filtered through a layer of calico, the filter is washed with hot water 85 ^o C.

The filtrate is evaporated at 75 ^o C on an evaporation cup under vacuum to 300 ml (pH 6.6 - 7.6).

To evaporated and cooled to 30 ^o C the filtrate add 96% alcohol 1: 1 and put on crystallization in a refrigerator at 4 ^o C for 5 days. A precipitate of crude inulin is light gray in color.

The wet sediments of crude inulin from juice and aqueous extraction from the pomace are separated by filtration and combined. The precipitate is dissolved in a minimum volume of hot water at 75 ^o C. The solution is filtered through a cotton cloth. The filter is washed with 20 ml of hot water (80 ^o C).

The solution is cooled to 45 ^o C and passed at a rate of 1 drop per second through a column with anion exchange resin in the OH ^- form. The column is washed with water at 45 ^o C, collect 350 ml of eluate (pH 10.2).

 The column is taken with a diameter of 3 cm, a height of 20 cm. The mass of dry anion exchange resin is 50 g, which is soaked in 1 N. a solution of sodium hydroxide, washed with distilled water by decantation, then loaded into a column in the form of a suspension in water and washed until neutral with phenolphthalein.

125 g of alumina is added to the eluate, heated on an electric stove with an asbestos mesh at 75 ^° C. with constant stirring for 30 minutes. Filter the hot mixture through a layer of calico. The filter is washed with 100 ml of hot water at 75 ^o C. Collect 350 ml of the filtrate.

75 g of calcium carbonate are added to the filtrate, heated at 75 ^° C. with constant stirring for 30 minutes, filtered hot through a layer of calico. The filter is washed with 75 ml of hot water (75 ^o C). 350 ml of the filtrate (pH 9.22) is collected, which is immediately heated to 75 ^° C. and re-filtered under vacuum through a 20 g alumina layer, placed on a Buchner funnel between paper filters and pre-washed with hot water. The filter at the end of the filtration is washed with 30 ml of hot water at 75 ^o C.

The filtrate is successively passed at a rate of 1 drop per second, first through a cation exchanger in the H ⁺ form at 45 ^° C. and washed with 50 ml of water, then immediately passed through anion exchange resin in the OH ^- form and washed with 50 ml of water. 350 ml of eluate are collected (pH 6.5 - 7.3). A colorless or slightly yellow opalescent solution is obtained. (Dry ion exchange resin mass 50 g, column diameter 3 cm, column height 20 cm). In the presence of yellowness in the solution, it is discolored with 50 g of charcoal activated carbon at 60 ^o C for 30 minutes Coal is separated by filtering a hot solution through a dense paper filter, passing the solution several times through the same filter.

To the resulting solution was added 96% alcohol 1: 3 and refrigerated at 4 ^° C for 24 hours. The precipitate was dissolved in a minimum volume of hot (80 ^° C) water and reprecipitated from the aqueous solution with 96% alcohol 1: 3 and likewise put on crystallization.

The precipitated inulin precipitate is filtered through a paper filter, washed twice with 85% alcohol in 20 ml filters, the precipitate is transferred to 96% alcohol and shaken with 50 ml of alcohol for 30 minutes. The precipitate was filtered and dried in air at room temperature on a food film substrate. Inulin is ground in a mortar to obtain 18 g of chromatographically pure snow-white inulin powder with a moisture content of 10% and specific rotation [α] _D = -36.5 ^o .

 The yield of air-dry inulin is 7.4% (anhydrous 6.7%) based on dry raw materials (with tuber moisture 75.7%). The inulin yield from the content in the raw material (7.7% of inulin found in dry tubers) is 87% for anhydrous inulin. Inulin purity was controlled by paper chromatography.

Example 2. Take 250 g of dried and pre-chopped Jerusalem artichoke tubers, pour 1500 ml of hot water, leaving to swell for 12 hours at 70 ^° C, then heat at 80 ^° C for 60 minutes and squeeze it through a layer of calico in hot form. Then, water is extracted three more times in portions of 1000 ml at 80 ^° C for 40 minutes. The hot extracts are combined, 100 g of calcium carbonate are added, heated for 60 minutes at 80 ^° C, filtered hot through a layer of calico, the filter is washed with 50 ml of hot water (85 ^° C). The filtrate was evaporated at 75 ^° C to 300-400 ml, cooled to 30 ^° C, 96% alcohol 1: 1 was added and allowed to crystallize in a refrigerator at 4 ^° C for 5 days. The precipitate of crude gray inulin is gray.

 Further purification of inulin is carried out in the same way as for the combined precipitation in example 1.

After cleaning and drying, inulin is crushed in a mortar and 17.5 g of chromatographically pure snow-white inulin powder with a moisture content of 10% and specific rotation [α] _D = -36.5 ^{o are} obtained.

 The yield of air-dry inulin is 7.8% (anhydrous 7%) based on dry tubers with a moisture content of 10%. The inulin yield from the content in raw materials (7.7% of inulin in dry tubers was found) is 90.9%. The purity of inulin is controlled by chromatography on paper.

List of references
1. Kochetkov N.K., Bochkov A.F. et al. Chemistry of carbohydrates. Fruktany.- P.535 - 536. M .: Chemistry. - 1967. -672 p.

 2. Methods of carbohydrate chemistry. G.O. Aspinal, E.A. Hurst. Inulin. Getting from dahlia tubers. Per. from English, ed. N.K. Kochetkova. Ed. World, 1967, S.370 - 371.

 3. Official Monographs, USP XXII, "Inulin", p. 700 - 702.

 4. Mino Joshiki, Tsutsui Satomi, Ota Nagayo. Separation of actylated inulin by reversed-phase high performance liquid chromatography. [Osaka Col. Pharm. , Kawai-2-10-65, Matsubara, Osaka 580, JP]. "Chem. And Pharm. Bull", 1985; 33, N 8, 3503-3506.

 5. Knyazev Yu. A., Nikberg II. Diabetes. Fructose. - S. 78. M: Medicine, 1989. - 143 p.

 6. Bjorn Ingelman (Swed), An inulin sulfuric acid ester with anticoagulant actiwity, Frkiv. Kemi. Mineral Geol. 24 B, No 5, 4pp (1946).

 7. Cooper, Peter Dodd (Australion National University), PCT Int. Appl. WO 87 02, 679 (Cl. CO 8B 37/18) 07 May 1987.- Inulin for immunoterapy of tumors, infections and immune sistem disorders and method for its preparathions.

 8. Iwasaki, Keiji; Ajsaka, Katsumi. Hemoglobin-inulin conjugated as a oxygen carriers for blood substitute. Biochem. Biophis Res. Commun. 1983, (2), 513-518.

 9. Cooper, Peter D; Carter Margrit (Australia), Anti-complementary action of polymorfic "solubility forms" of particulate inulin. Mol. Immunol. 1986, 23 (8), 895-901.

 10. Lazurevsky G.V., Terentyeva I.V., Shamshurin A.A., D-fructose from dahlia tubers. // Practical work on the chemistry of natural compounds. M .: Higher school. 1966. S. 68-69.

 11. Auth. St. USSR N 1214104, class A 61 K 35/78, 3785963 / 28-14, 08.20.94, VNIIKhTLS and the pilot plant VNIIKhTLS, V.V. Zimchenko, P.P. Khvorost, N.F. Komissarenko, etc.

 12. Auth. St. USSR, Particleboard, AG 1709612 A1, A 61 K 35/78, 1984, 4808959/14, 03/30/90, Institute of Organ. Chem. Academy of Sciences of the Kyrgyz SSR and chem. farm. Frunze factory "Frunzebiofarm", K. Turdumambetov, Yu.V. Nemaltsev, N.V. Plekhanova and others. N 1214104, (54, 57) A method for producing inulin.

 13. B.J. D. Meuse, Inulin in the green alga Batophora oerstedi J. Ag, Dasycladales). "Acta Botanica Neerlandica", 1963, 12, 315-318.

 14. Auth. St. USSR, N 487118, class. C 1313/00, claimed. 03.03.73 N 1891936, publ. 02/26/76. Fedotkin I.M., Gerasimenko A.A., Bobrovnik L.D. et al. [Kiev Technological Institute of Food Industry]. The method of obtaining inulin.

 15. Golubev V.N., Volkova N.V., Kushalakov Kh.M. Jerusalem artichoke. Composition, properties, processing methods, applications. M., 1995.

 16. RF patent, 2001621, A 61 K 35/78, 31/715, C 13 K 11/00, 5044672/14, 05.29.92. Borisenko V.G., Korolev V.D., Pedanov F.R. and others. A method of producing inulin.

 17. Warteresienicz Mariusz. Hydroliza glikofruktozanow topinamburu (Helianthus tuberosus L.). Ann. Univ. M. Curie-Sklodowska. 1962. C.16, 163-188.

 18. Molchanov G.I., Molchanova L.P., Gulko N.M. and others. Edible healing plants of the Caucasus. Rostov-on-Don. Publishing House of Rostov University. 1989 - S. 282.

 19. Kakhana V.M., Shilina N.I. On the accumulation of inulin in Jerusalem artichoke tubers. // Materials of the IV conference of young scientists of Moldova. 1964. Section of physiology, biochemistry and plant genetics. - Chisinau. - 1966. - S.60 - 63.

Claims (4)

1. A method of producing inulin from Jerusalem artichoke tubers (Helianthus tuberosus L.) by obtaining extracts, purification on ion exchangers, precipitation of inulin, characterized in that juice and aqueous extracts from squeezes are obtained from fresh tubers, the primary purification of juice diluted with water 1: 1 and aqueous extracts from pomace by treatment with calcium carbonate at 85 ^o C for 60 min, the remaining particles of raw materials and calcium carbonate are separated, the filtrates are evaporated, crude inulin is crystallized at 4 ^o C in the refrigerator for 5 days from aqueous extracts After adding 1: 1 alcohol from diluted juice, separating inulin precipitates, dissolving the combined precipitates in hot water at 75 ^o C, additionally carry out purification using anion exchange resin, aluminum oxide, calcium carbonate, followed by purification using cation exchange resin and anion exchange resin, followed by crystallization and reprecipitation from a water-alcohol mixture 1: 3 at 4 ^o C for 24 hours in the refrigerator, the product is dried and crushed. 2. A method of producing inulin from Jerusalem artichoke tubers (Helianthus tuberosus L.) by obtaining extracts, purification on ion exchangers, precipitation of inulin, characterized in that aqueous extracts from dry crushed Jerusalem artichoke tubers are obtained by mixing them with hot water in a ratio of 1: 6, withstand 12 h for swelling at 70 ^o С and extractives are extracted at 80 ^o С, water extraction is separated and extraction is carried out three more times for 40 minutes in a ratio of 1: 4, followed by combining the extracts, then they are treated with calcium carbonate at 80 ^o С for 60 min, filtered, the filtrate was evaporated, crude inulin was crystallized at 4 ^° C in the refrigerator for 5 days after adding 1: 1 alcohol, the inulin precipitate was separated, it was dissolved in hot water at 75 ^° C, additionally, it was purified using anion exchange resin, aluminum oxide, calcium carbonate with further purification using cation exchange resin and anion exchange resin, followed by crystallization and reprecipitation from a water-alcohol mixture 1: 3 at 40 ^o C for 24 hours in a refrigerator, the product is dried and crushed. 3. The method according to p. 1 or 2, characterized in that the additional purification of the aqueous solution of crude inulin is carried out by passing the solution through anion exchange resin at 45 ^o C, followed by heating the eluate with alumina, and then with calcium carbonate at 75 ^o C for 30 minutes separating them and filtering the hot solution under vacuum through a layer of aluminum oxide. 4. The method according to claim 1 or 2, characterized in that the further purification of inulin is carried out by passing an aqueous solution at 45 ^{° C.} sequentially through cation exchange resin in the H ⁺ form and through anion exchange resin in the OH ^- form, subsequent crystallization is carried out at 4 ^{° C.} a refrigerator for 24 hours with the addition of 96% alcohol 1: 3 by volume to the eluate with a twofold precipitation of inulin from an aqueous solution of 96% alcohol 1: 3 at 4 ^° C for 24 hours, filtering and shaking the precipitate in 96% alcohol for 30 min and drying the product is carried out in air on a substrate of food film.