RU2098137C1 - Method of contact lens cleansing - Google Patents

Abstract

FIELD: medicinal engineering, ophthalmology. SUBSTANCE: method involves the use of the immobilized proteolytic enzymes and the modified nucleuses. Firstly, immobilized enzymes as bacterial proteinases (activity is 120-150 ПЕ/ml), 3-6 g; polyalkylene oxide, 2-5 g; and water, the balance are used followed by use of the modified nucleases solution. EFFECT: enhanced effectiveness of cleansing, increased rate, antiviral protection safety.

Description

 The invention relates to medicine, and more particularly to ophthalmology.

 A known method of cleaning contact lenses (French application N 2400906, CL A 6 L 13/00, 1979). According to this method, contact lens disinfection and cleaning compositions contain endiol, which may be ascorbic, isoascorbic, dioximaleic, dioxifumaric, reducing acid, its salt or derivative, and an oxidizing agent in the form of water-soluble alkali metal percarbonate peroxide, alkaline earth metal peroxide, or peroxide hydrogen. The compositions can be used mainly for cleaning contact, mainly flexible lenses.

 However, this method has a significant drawback: lack of effectiveness, the duration of cleaning and the lack of a guarantee against infection of various viruses (hepatitis A, B, AIDS).

 A known method of cleaning contact lenses (UK application N 2003033, 1979).

 A method for disinfecting and cleaning contact lenses involves the use of a redox system that contains endiol, which are ascorbic acid, isoascorbic acid, dioximaleic acid, dioxifumaric acid or 2,3-dioxo-2-cyclopentenone-1 or its derivatives, and an oxidizing agent in the form water-soluble peroxide (alkali metal processing, alkaline earth metal peroxide, peroxide) alkaline metal processing, alkaline earth metal peroxide, urea peroxide or hydrogen peroxide Yes.

 However, this method has a significant drawback: lack of effectiveness, the duration of cleaning and the lack of a guarantee against infection of various viruses (hepatitis A, B, AIDS).

 The technical task of the invention is to increase the efficiency of cleaning, increasing the speed of cleaning, while providing antiviral protection.

 The specified technical problem is provided by the fact that in the method of cleaning contact lenses, which consists in the use of chemicals, immobilized proteolytic enzymes and modified nucleases are used as a substance, while immobilized enzymes in the form of bacterial proteinases with activity from 120 to -150 PE / milliliter are initially used -5 -8 g. 1.4 polyalkylene oxide -2 -5 g, the rest is water, after which a solution of modified nucleases 200-250 mg is used.

 A lot of experimental work was done to determine the intervals of the ingredients.

 Bacterial proteinases cannot be less than 5 g, because in the case of large protein contaminants, the number of enzymes and their activity may be insufficient for the desired effect, and more than 8 g, because excess leads to unjustified consumption of enzymes.

 2,4 polyalkylene oxide can not be less than 2 g, because a smaller amount does not guarantee complete immobilization of proteinases on the carrier, and more than 5 g, because an excess of the substance does not carry additional beneficial properties.

 Modified epelmaz in solution cannot be less than 200 m, because in experiments it was shown that a dose reduction increases the risk of viruses remaining, and more than 250 m, because this is not justified in view of the adequacy of the dose.

 To clean contact lenses, a kit consisting of two bottles with detergents is used, as well as a plastic container with a rotating rotor, the cells of which allow you to freely fix separately left and right lenses.

 Bottle No. 1 contains 10 ml of a solution of immobilized bacterial proteinases with a specific activity of 120 NE / ml and chlorhexysine. 0.02 bottle No. 2 contains a complex of modified nucleases with a total RNA-DNA and DNA-zine activity of 50 EA / mg.

 The detergent composition includes immobilized proteinases with an activity of 60 NE / ml, chlorhexysine (bottle No. 1), a nuclease complex with a total activity of 120 EA / mg. The selected activity of proteinases is minimally sufficient to guarantee the necessary enzymatic cleavage of protein contaminants.

 The lens cleaning is carried out as follows: contact lenses after wearing are placed in the rotor retainers of the container. 10 ml of solution from bottle N 1 is poured into the container.

 The first stage of purification from protein contaminants lasts 2-4 hours with periodic slow rotation of the container rotor. After the completion of the 1st stage, the spent solution is poured out, replacing it with a solution with added nucleases (bottle N 2).

 Stage 2 antiviral lens treatment continues with a slow periodic rotation of the rotor. The spent solution is poured by pouring a neutral solution into the container.

 The entire cleaning cycle takes 4-6 hours. After the cycle is completed, the lenses are considered cleaned and suitable for everyday wear.

 Example 1. Patient A. 35 years. Unpleasant sensations in the eyes in the form of "soft fog", irritation of the mucosa are disturbing. Diagnosis: moderate myopia in both eyes. The patient has been using hydrogel lenses (manufactured by Johnson and Johnson) for 2 years. The lens is periodically washed with boiled warm water, hydrogen peroxide.

 The examination found: a mixed injection of eyeballs, edema of the corneal epithelium, limited areas of de-epithelization.

 A study of contact lenses under a microscope showed the following: the right and left lenses had multiple foci of contamination in the form of conglomerate deposits.

 In order to restore the optical properties of the lenses, they were processed by the proposed method.

 After the completion of the process, the surface of the lenses became more shiny, the shade of visibility disappeared, the elastic properties did not change. Conglomerate deposits are absent.

 Example 2. Patient L. 20 years. Complaints of discomfort in the eyes associated with wearing lenses. Disturbed by a change in vision. Diagnosis: high myopia in both eyes. Hydrogel contact lenses (manufactured by Johnson & Johnson) have been used for 1.5 years. Washes periodically in boiled water. Keeps in a container with boiled water. Examination revealed pericorneal injection of eyeballs.

 A study of the lenses showed: the presence of multiple conglomerates of detritus over the entire surface of both lenses.

 It is recommended to use detergent components.

 After washing the lenses according to the proposed method, both lenses were examined under a microscope. The study showed a complete absence of conglomerates on their surface. After cleaning the lenses according to the specified methodology, wearing the lenses as usual did not cause concern for the patient.

The use of the invention allows:
conduct effective cleaning of lenses from protein contaminants due to their hydrolytic cleavage;
eliminate the risk of infection with the AIDS virus as a result of lens treatment with a solution of highly effective nucleases;
reduce discomfort and reduce the risk of complications associated with wearing contaminated lenses;
increase lens life.

Claims (1)

 A method for cleaning contact lenses, comprising treating the latter with a solution containing an enzyme preparation, characterized in that immobilized proteolytic enzymes and modified nucleases are used as the enzyme preparation, while initially the lenses are treated with a solution containing immobilized proteolytic enzymes in the form of bacterial proteinases with an activity of 120 150 PE / ml 5 8 g, 1,4-polyalkylene oxide 2 5 g, water is the rest, after which the lenses are treated in a solution of modified nucleases with a concentration of 2 , 2.5 mg / ml.