RU2050352C1 - Diethyl aminomethyl urea as antimicrobic additive for cutting liquids - Google Patents

Abstract

FIELD: production of cutting liquids. SUBSTANCE: novel compound diethyl aminomethyl urea having essential formula (C₂H₅)₂NCH₂NH-CO-NH₂ is proposed as antimicrobic additives for cutting liquids. EFFECT: improves quality of desired product. 1 tbl

Description

The invention relates to a new chemical compound, specifically diethylaminomethylurea of the formula (C ₂ H ₅ ) ₂ NCH ₂ NH-CO-NH ₂ , which may find application as an antimicrobial additive to cutting fluids.

 Cutting fluids used in various metalworking operations are exposed to various microorganisms, most often aerobic and anaerobic bacteria, fungi and yeast. The growth of microorganisms in the coolant leads to a deterioration in their technological, physico-chemical and sanitary-hygienic properties. For this reason, the life of the coolant is significantly reduced, which leads to large economic losses.

The main way to combat the microbiological lesion of the coolant is the use of antimicrobial additives. It is known to use various chemical compounds (triazine compounds, phenols quinoline derivatives) as antimicrobial additives to the coolant [1-3]
However, most of these compounds are not sufficiently active, have a narrow spectrum of antimicrobial activity, and microorganisms quickly adapt to them.

 The aim of the invention is to improve antimicrobial coolant.

 This goal is achieved by the introduction of diethylaminomethylurea into the composition of emulsion coolant.

The specified compound is obtained by the interaction of urea, paraform and diethylamine in a known manner [4] at a temperature of 40-50 ^about C for 4 hours

PRI me R. To a mixture of 30 g (0.5 mol) of urea and 73 g (1 mol) of diethylamine, 30 g (0.3 mol) of paraform was added portionwise. The resulting mixture was stirred at 40-50 ^{° C} for 4 hours. After standing over anhydrous sodium sulfate, the product was distilled in vacuo. Received 67.7 g of product. The target product is a straw-colored liquid that has dissolved well in water. Yield 91.5% bp 138-140 ^{° C} (0.2 mm) n _D ²⁰ 1,4698, d ^{₂₀ April} 0,9793, MR _D Found 41.54% Calculated 40.87% Found: C, 50.03; H 10.45; N, 29.70. C ₆ H ₁₅ N ₃ O. Calculated C 49.65; H 10.34; N, 28.96.

The IR spectrum of diethylaminomethylurea contains absorption bands: 1660 cm ^-1 , corresponds to stretching vibrations of C = 0 bonds, 1631 cm ^-1 NH ₂ bonds, 3350 cm ^-1 NH bonds, 1555 cm ^-1 C-NH bonds and 1250 cm ^-1 C-NH ₂ communication.

In the NMR spectrum (δm, d): 1.00 t (6H, CH ₃ ); 2.50 to (6H, CH ₂ ) ₃ ); 4.36 s (3H, HN-NH ₂ ).

Diethylaminomethylurea was studied as a biocidal additive to the emulsion cutting fluid Azerol-1 [5]
The proposed compound was introduced into a 5% working emulsion coolant Azerol-1. As a control, a freshly prepared 5% coolant emulsion Azerol-1, not protected by a biocide, served. The widely used domestic preparation “Vazin” was used as a comparison base.

The antimicrobial effectiveness of diethylaminomethylurea is determined by the method of zonal diffusion according to GOST 9.085.78 using the following microorganisms:
Bacteria: Escherichia coli
Mycobacterium phlei
Pseudomonas aeruginosa
Staphuloccus aurcus
Mushrooms:
Aspergillus niger
Chaetomium geobosum
Cladosporium gossipicola
Cladosporium resinae
Penicillium chrysogenum
Trichoderma viride
Trichoderma condidi
and yeast-like microorganisms Candida tropicalis.

 Meat-peptone agar (MPA) was used to grow bacterial cultures, and wort-agar was used for fungi and yeast.

For testing, nutrient medium (MPA) is poured into Petri dishes in the amount of 20-25 ml and then it is allowed to solidify. Sowing of microorganisms was carried out on the surface of the nutrient medium. Then, 4 holes were made on the surface of the medium using a sterile drill with a diameter of 10 mm and a depth of 4-5 mm. Next, 0.2 ml of coolant with the indicated compound was added to these wells. Then the petri dish was placed in a thermostat and maintained for 2 days using bacteria and 3-4 days for fungi at a certain humidity and a temperature of 30 ± 2 ° ^C. The efficacy of antimicrobial activity determined dietilaminometilmocheviny largest diameter zone of inhibition of microbial growth. Similar tests were performed with Vazin as part of the coolant Azerol-1. The test results are shown in the table.

 As the data in the table show, diethylaminomethylurea effectively inhibits the growth of microorganisms in the coolant Azerol-1. At the same time, the biocidal concentration of this compound is less than when using Vazin. So, Vazin in 0.2% concentration does not inhibit the growth of both bacterial and fungal microflora. The proposed compound not only at a concentration of 0.2% but also at a concentration of 0.1% shows high efficiency.

 The advantage of the proposed compound lies in the fact that at concentrations of 0.2 and 0.1%, unlike Vazin, it inhibits the growth of yeast-like fungi Candida tropicalis.

 Based on the test results, diethylaminomethylurea can be recommended as an additive to prevent bio-damage of emulsion coolants.

Claims (1)

Diethylaminomethylurea formula
(C ₂ H ₅ ) ₂ NCH ₂ NH-CO-NH ₂
as an antimicrobial additive to cutting fluids.

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