RU2033173C1 - Method for manufacture of preparation from medical leeches to produce antithrombotic, thrombolythic, antiatherosclerotic and hypotensive effects - Google Patents

Abstract

FIELD: medicine; pharmacology, particularly, methods for manufacture of biologically active medicines. SUBSTANCE: essence of this method resides in repeatedly washing off leeches in distilled water and physiologic salt solution. Further steps are freezing, lyophilizing, separating skin tissue by special techniques, grinding to powder and using resultant product in tests on animals either in the form of powder or suspension in physiologic salt solution. Disclosed preparation may be administered per os or subcutaneously, intramuscularly or intravenously in the form of aqueous extracts. EFFECT: higher biological activity of end product.

Description

The invention relates to methods for biologically active substances and can be used in hematology, medical industry, veterinary medicine, medicine and in laboratory practice,
A known method of obtaining a medicinal product from a medical leech that has only a thrombolytic effect, but does not have an antithrombolytic, antiatherosclerotic and hypotensive effect.

 The aim of the invention is to obtain a medicinal product from a medical leech with antithrombotic, thrombolytic, antiatherosclerotic and antihypertensive effects.

 The goal is achieved by the fact that medical leeches are washed with water and physiological saline, frozen and freeze-dried to increase the biological activity of the drug using a special technique, the lyophilized product is freed from the skin, ground into powder and used.

 The following materials illustrate goal achievement.

Take 20 units of medicinal leeches, washed repeatedly with water and then with saline, frozen at ^-70 ° C and freeze-dried. The average weight of one dried leech is 0.35 ± 0.05 g. After removal of the skin tissue, it is ground into powder and weighed. Its mass is 0.15 ± 0.03 g per one leech. Out of 20 leeches, an average of 2.5-3.0 g of therapeutic powder is obtained.

There is a high solubility of the drug in physiological saline. The clotting time of fibrinogen with thrombin in the presence of a solution (antithrombin activity) increases by 70%; the time of recalcification of blood plasma is almost doubled; destabilase activity is one and a half times; antitryptic activity is also doubled; antichymotriptic activity by 70%
When rats are intravenously injected with an extract prepared at the rate of 5 mg of powder in 1 ml of physiological saline, the degree of thrombosis is 50 ± 5% (100% control) with a 24-hour interval between administration of the drug and human blood serum, which stimulates the formation of a blood clot on the background of stasis of the jugular vein rats. With the oral route of administration, the degree of thrombosis is 75 ± 5% (n 8, p <0.01).

 With the intravenous administration of an extract obtained from 20 mg of the drug, the degree of thrombolysis is 75 ± 5% (n 8; p <0.01), and with the oral administration of the same dose, the degree of thrombolysis is 70 ± 5% (n 12, p <0 , 01). In the control group of animals, the degree of thrombolysis is 20 ± 5% (n 3) due to spontaneous thrombolysis.

 With daily oral administration to rats on an atherogenic diet (according to Wilgram in the modification of Bazazyan) and having pronounced signs of atherosclerosis, 50 mg of the drug (n 10) was administered for 10 days. As a result, the phenomena of regression of atherosclerosis were detected, which was judged by a decrease in the area of lipid swelling in the thoracic and abdominal aorta compared with the control group of rats (n 10) treated with physiological saline instead of a therapeutic drug.

 The antihypertensive effect was detected by oral administration of a suspension of 40 mg of the drug in 1 ml of saline to SHR rats (spontaneously hypertensive). The initial pressure level in the tail vein was 160 ± 5 mm Hg. After a single injection of the drug the next day, a decrease in pressure to 135 ± 5 mm Hg was recorded. on the second day after administration of the drug, it increased to 150 ± 5 mm, and then on the fifth day it returned to the initial level (n 12).

Claims (1)

 A METHOD FOR PRODUCING A DRUG FROM A MEDICAL LIE, HAVING ANTITROMBOTIC, THROMBOLYTIC, ANTIATHEROSCLEROTIC AND HYPOTENSIVE ACTION, which consists in rinsing the leech with saline and triturated water, and then dissolving it with physiologically dried powder.