RU2020135557A - Method for conducting enzyme-linked immunosorbent assay using double specific binding technology for detecting antibodies against COVID-19 and test system - Google Patents

Method for conducting enzyme-linked immunosorbent assay using double specific binding technology for detecting antibodies against COVID-19 and test system Download PDF

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RU2020135557A
RU2020135557A RU2020135557A RU2020135557A RU2020135557A RU 2020135557 A RU2020135557 A RU 2020135557A RU 2020135557 A RU2020135557 A RU 2020135557A RU 2020135557 A RU2020135557 A RU 2020135557A RU 2020135557 A RU2020135557 A RU 2020135557A
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Prior art keywords
test system
specific binding
linked immunosorbent
immunosorbent assay
antibodies against
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RU2020135557A
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Russian (ru)
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RU2020135557A3 (en
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Артур Александрович Исаев
Екатерина Алексеевна Померанцева
Светлана Олеговна Жикривецкая
Яна Владиславовна Софронова
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Общество с ограниченной ответственностью "Центр Генетики и Репродуктивной Медицины "ГЕНЕТИКО"
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Priority to RU2020135557A priority Critical patent/RU2020135557A3/en
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Claims (2)

1. Способ проведения иммуноферментного анализа по технологии двойного специфического связывания для выявления антител, специфичных к коронавирусу SARS-COV2, в биологическом образце, с применением тест-системы, предусматривающий проведение реакции на выявление суммарных антител - иммуноглобулинов А, М и G, без предварительного разведения биологического образца, при этом в качестве конъюгатов используют биотинилированный S-RBD белок SARS-COV2 и полимеризованную пероксидазу со стрептавидином.1. Method for conducting enzyme-linked immunosorbent assay using the technology of double specific binding to detect antibodies specific to SARS-COV2 coronavirus in a biological sample, using a test system, involving the reaction to detect total antibodies - immunoglobulins A, M and G, without preliminary dilution a biological sample, while biotinylated S-RBD SARS-COV2 protein and polymerized peroxidase with streptavidin are used as conjugates. 2. Тест-система для проведения способа по п. 1, включающая раствор конъюгата биотинилированного S-RBD белка SARS-COV2, раствор конъюгата полимеризованной пероксидазы со стрептавидином, фосфатно-солевой буфер, фосфатно-солевой буфер с 0,1% Твин-20 (ФСБ-Т), ФСБ-Т с 4% БСА (бычьего сывороточного альбумина), ФСБ-Т с 1% БСА, раствор 3,3',5,5'-тетраметилбензидин ТМБ, 0,5М серная кислота.2. Test system for carrying out the method according to claim 1, including a solution of a biotinylated S-RBD conjugate of the SARS-COV2 protein, a solution of a polymerized peroxidase conjugate with streptavidin, phosphate-buffered saline, phosphate-buffered saline with 0.1% Tween-20 ( FSB-T), FSB-T with 4% BSA (bovine serum albumin), FSB-T with 1% BSA, 3,3',5,5'-tetramethylbenzidine TMB solution, 0.5M sulfuric acid.
RU2020135557A 2020-10-29 2020-10-29 Method for conducting enzyme-linked immunosorbent assay using double specific binding technology for detecting antibodies against COVID-19 and test system RU2020135557A3 (en)

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RU2020135557A3 RU2020135557A3 (en) 2022-04-29

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