RU2019143343A - METHOD FOR IN VITRO REPRODUCTION OF STAMP VARIETIES OF RASPBERRY - Google Patents

METHOD FOR IN VITRO REPRODUCTION OF STAMP VARIETIES OF RASPBERRY Download PDF

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Publication number
RU2019143343A
RU2019143343A RU2019143343A RU2019143343A RU2019143343A RU 2019143343 A RU2019143343 A RU 2019143343A RU 2019143343 A RU2019143343 A RU 2019143343A RU 2019143343 A RU2019143343 A RU 2019143343A RU 2019143343 A RU2019143343 A RU 2019143343A
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RU
Russia
Prior art keywords
raspberry
varieties
stamp
passages
nutrient medium
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RU2019143343A
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Russian (ru)
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RU2019143343A3 (en
RU2751250C2 (en
Inventor
Елена Олеговна Видягина
Вадим Георгиевич Лебедев
Константин Александрович Шестибратов
Original Assignee
Федеральное государственное бюджетное образовательное учреждение высшего образования "Пущинский государственный естественно-научный институт" (ПущГЕНИ)
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Priority to RU2019143343A priority Critical patent/RU2751250C2/en
Publication of RU2019143343A3 publication Critical patent/RU2019143343A3/ru
Publication of RU2019143343A publication Critical patent/RU2019143343A/en
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Publication of RU2751250C2 publication Critical patent/RU2751250C2/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Claims (1)

Способ эффективного размножения in vitro штамбовых сортов малины, включающий чередование четырех пассажей на питательной среде MS с добавлением никотиновой кислоты 0,5 мг/л, глицина 2,0 мг/л, аскорбиновой кислоты 55,7 мг/л, инозитола 100 мг/л, пиридоксина 0,1 мг/л, тиамина 0,1 мг/л, агар-агара 10,8 г/л, сахарозы 30 г/л, отличающийся тем, что первые и второй пассажи состава питательной среды дополнительно включал 6-бензиламинопурин 0,7 мг/л и зеатин 0,1 мг/л (Среда 1) при интенсивности освещения 1500-2000 люкс, третий и четвертый пассажи состава питательной среды дополнительно включал 6-бензиламинопурин 0,6 мг/л и индолилмасляную кислоту 0,1 мг/л (Среда 2) при интенсивности освещения 800-1000 люкс.A method of effective in vitro propagation of standard raspberry varieties, including the alternation of four passages on the MS nutrient medium with the addition of niacin 0.5 mg / l, glycine 2.0 mg / l, ascorbic acid 55.7 mg / l, inositol 100 mg / l , pyridoxine 0.1 mg / l, thiamine 0.1 mg / l, agar-agar 10.8 g / l, sucrose 30 g / l, characterized in that the first and second passages of the nutrient medium composition additionally included 6-benzylaminopurine 0 , 7 mg / l and zeatin 0.1 mg / l (Wednesday 1) at an illumination intensity of 1500-2000 lux, the third and fourth passages of the nutrient medium composition additionally included 6-benzylaminopurine 0.6 mg / l and indolylbutyric acid 0.1 mg / l (Wednesday 2) at an illumination intensity of 800-1000 lux.
RU2019143343A 2019-12-24 2019-12-24 Method of in vitro reproduction of standard raspberry cultivars RU2751250C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2019143343A RU2751250C2 (en) 2019-12-24 2019-12-24 Method of in vitro reproduction of standard raspberry cultivars

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RU2019143343A RU2751250C2 (en) 2019-12-24 2019-12-24 Method of in vitro reproduction of standard raspberry cultivars

Publications (3)

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RU2019143343A3 RU2019143343A3 (en) 2021-06-24
RU2019143343A true RU2019143343A (en) 2021-06-24
RU2751250C2 RU2751250C2 (en) 2021-07-12

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114073226A (en) * 2021-11-23 2022-02-22 河北农业大学 Inoculation method for tissue culture and rapid propagation of raspberry petioles
CN115152629A (en) * 2022-07-26 2022-10-11 辽宁省果树科学研究所 Raspberry tissue culture method
CN115968778A (en) * 2022-10-19 2023-04-18 北京大学现代农业研究院 Method for in vitro regeneration culture of raspberries

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114073226A (en) * 2021-11-23 2022-02-22 河北农业大学 Inoculation method for tissue culture and rapid propagation of raspberry petioles
CN114073226B (en) * 2021-11-23 2023-10-27 河北农业大学 Inoculation method for tissue culture and rapid propagation of raspberry leaf stalks
CN115152629A (en) * 2022-07-26 2022-10-11 辽宁省果树科学研究所 Raspberry tissue culture method
CN115968778A (en) * 2022-10-19 2023-04-18 北京大学现代农业研究院 Method for in vitro regeneration culture of raspberries

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Publication number Publication date
RU2019143343A3 (en) 2021-06-24
RU2751250C2 (en) 2021-07-12

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