RU2019108525A - Method for the diagnosis of vascular bacteriosis of cruciferous plants by dot-immunoassay - Google Patents

Method for the diagnosis of vascular bacteriosis of cruciferous plants by dot-immunoassay Download PDF

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RU2019108525A
RU2019108525A RU2019108525A RU2019108525A RU2019108525A RU 2019108525 A RU2019108525 A RU 2019108525A RU 2019108525 A RU2019108525 A RU 2019108525A RU 2019108525 A RU2019108525 A RU 2019108525A RU 2019108525 A RU2019108525 A RU 2019108525A
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Russia
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dot
serum
bacteriosis
immunoassay
vascular
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RU2019108525A
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Russian (ru)
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RU2019108525A3 (en
RU2736806C2 (en
Inventor
Михаил Андреевич Кузнецов
Анатолий Анисимович Щербаков
Виктор Михайлович Скорляков
Светлана Валерьевна Савина
Сергей Владимирович Иващенко
Михаил Николаевич Киреев
Ольга Сергеевна Ларионова
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Федеральное государственное бюджетное образовательное учреждение высшего образования "Саратовский государственный аграрный университет имени Н.И. Вавилова"
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Priority to RU2019108525A priority Critical patent/RU2736806C2/en
Publication of RU2019108525A3 publication Critical patent/RU2019108525A3/ru
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (1)

Способ диагностики сосудистого бактериоза крестоцветных методом дот-иммуноанализа, выполняемый на нитроцеллюлозной мембране с использованием высокоспецифической сыворотки, содержащей антитела к выявляемому возбудителю, и окрашивании раствором коньюгата коллоидного золота с белком А стафилококка по методу Г. Френса, отличающийся тем, что используется иммуноспецифическая кроличья сыворотка на основе антигена клеточной стенки X. campestris, при получении сыворотки в качестве адьюванта используется химическая полиэлектролитная субстанция, состоящая из 0,05% раствора полиазолидинаммония, модифицированного гидрат-ионами галогенов, в физиологическом растворе, анализ проводится в варианте dot-ELISA препаратами растительных тканей или смывов с исследуемых поверхностей, с сывороткой, взятой в разведении 1:100; интенсивность окрашивания цветной реакции измеряется при помощи программы-денситометра Sorbfil TLC View.A method for diagnosing vascular bacteriosis of cruciferous plants by the dot-immunoassay method, performed on a nitrocellulose membrane using highly specific serum containing antibodies to the detected pathogen, and staining with a solution of colloidal gold conjugate with staphylococcus protein A according to the method of G. Frens, which is distinguished by the fact that it uses immunospecific serum based on the cell wall antigen of X. campestris, when obtaining serum, a chemical polyelectrolyte substance is used as an adjuvant, consisting of a 0.05% solution of polyazolidine ammonium, modified with halogen hydrate ions, in saline, the analysis is carried out in the dot-ELISA version with preparations of plant tissues or washes from the investigated surfaces, with serum taken at a dilution of 1: 100; the intensity of the color reaction staining is measured using the Sorbfil TLC View densitometer software.
RU2019108525A 2019-03-25 2019-03-25 Diagnostic technique for cruciferous vascular bacteriosis by dot-immunoassay RU2736806C2 (en)

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RU2019108525A RU2736806C2 (en) 2019-03-25 2019-03-25 Diagnostic technique for cruciferous vascular bacteriosis by dot-immunoassay

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RU2019108525A RU2736806C2 (en) 2019-03-25 2019-03-25 Diagnostic technique for cruciferous vascular bacteriosis by dot-immunoassay

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RU2019108525A3 RU2019108525A3 (en) 2020-09-25
RU2019108525A true RU2019108525A (en) 2020-09-25
RU2736806C2 RU2736806C2 (en) 2020-11-20

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Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2083090C1 (en) * 1995-06-29 1997-07-10 Московская сельскохозяйственная академия им.К.А.Тимирязева Method for selecting of cabbage plants resistant to mucous bacteriosis
RU2464573C1 (en) * 2011-02-21 2012-10-20 Учреждение Российской академии медицинских наук Научно-исследовательский институт эпидемиологии и микробиологии Сибирского отделения РАМН Instrument-free diagnostic technique for pseudotuberculosis

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RU2019108525A3 (en) 2020-09-25
RU2736806C2 (en) 2020-11-20

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Effective date: 20210326