RU2018102699A - A method for assessing the level of mineralizing potential of oral fluid in children - Google Patents

A method for assessing the level of mineralizing potential of oral fluid in children Download PDF

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RU2018102699A
RU2018102699A RU2018102699A RU2018102699A RU2018102699A RU 2018102699 A RU2018102699 A RU 2018102699A RU 2018102699 A RU2018102699 A RU 2018102699A RU 2018102699 A RU2018102699 A RU 2018102699A RU 2018102699 A RU2018102699 A RU 2018102699A
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level
oral fluid
mineralizing potential
mineralizing
potential
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RU2018102699A
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Russian (ru)
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RU2708090C2 (en
RU2018102699A3 (en
Inventor
Галина Ивановна Скрипкина
Юрий Борисович Никитин
Александр Прокофьевич Коршунов
Татевик Левоновна Бернецян
Original Assignee
федеральное государственное бюджетное образовательное учреждение высшего образования "Омский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ФГБОУ ВО ОмГМУ Минздрава России)
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Priority to RU2018102699A priority Critical patent/RU2708090C2/en
Publication of RU2018102699A publication Critical patent/RU2018102699A/en
Publication of RU2018102699A3 publication Critical patent/RU2018102699A3/ru
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (1)

Способ оценки уровня минерализующего потенциала ротовой жидкости, отличающийся тем, что слюну человека центрифугируют в течение 10 минут при скорости 3000 об/мин с последующим нанесением трёх капель надосадочной жидкости на химически чистое стекло и термостатированием при t = 37°С в течение 30-40 минут для исследования под микроскопом и фотографирования в течение 18-36 часов с последующим использованием программы анализа изображений «Image Tool 2.0» с целью определения расстояния между параллельными древовидными кристаллами образцов с последующим вычислением средней величины и сравнения с контрольными цифрами для определения уровня минерализующего потенциала ротовой жидкости, который может быть благоприятным при типе микрокристаллизации слюны с цифровым выражением 1 – 127,0 пикселей и не благоприятным при типе микрокристаллизации слюны с цифровым выражением 127,1 пикселей и более; и для диагностики риска развития и усугубления активности кариозного процесса у детей: высокий риск - при не благоприятном уровне минерализующего потенциала ротовой жидкости и низкий риск - при благоприятном уровне минерализующего потенциала ротовой жидкости. A method for assessing the level of mineralizing potential of an oral fluid, characterized in that the saliva of a person is centrifuged for 10 minutes at a speed of 3000 rpm, followed by applying three drops of the supernatant to chemically clean glass and thermostating at t = 37 ° C for 30-40 minutes for research under a microscope and photographing for 18-36 hours, followed by the use of the image analysis program “Image Tool 2.0” to determine the distance between parallel tree crystals of samples, followed by the calculation of the average value and comparison with control figures to determine the level of mineralizing potential of the oral fluid, which may be favorable for the type of microcrystallization of saliva with a digital expression of 1 - 127.0 pixels and not favorable for the type of microcrystallization of saliva with a digital expression of 127.1 pixels or more; and to diagnose the risk of development and aggravation of the activity of the carious process in children: high risk - with an unfavorable level of mineralizing potential of the oral fluid and low risk - with a favorable level of the mineralizing potential of the oral fluid.
RU2018102699A 2018-01-24 2018-01-24 Method for assessing level of mineralizing potential of oral fluid in children RU2708090C2 (en)

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Publications (3)

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RU2018102699A true RU2018102699A (en) 2019-07-24
RU2018102699A3 RU2018102699A3 (en) 2019-08-23
RU2708090C2 RU2708090C2 (en) 2019-12-04

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Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2413229C1 (en) * 2009-10-22 2011-02-27 Евгений Сергеевич Иощенко Method of predicting carious process in children

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RU2018102699A3 (en) 2019-08-23

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