RU2017408C1 - Method of estimation of plant salt-resistance - Google Patents

Method of estimation of plant salt-resistance Download PDF

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Publication number
RU2017408C1
RU2017408C1 SU4949800A RU2017408C1 RU 2017408 C1 RU2017408 C1 RU 2017408C1 SU 4949800 A SU4949800 A SU 4949800A RU 2017408 C1 RU2017408 C1 RU 2017408C1
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Russia
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resistance
salt
plants
plant
estimation
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Russian (ru)
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М.Д. Кушниренко
Г.П. Курчатова
О.А. Харчук
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Институт физиологии растений АН Республики Молдова
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Priority to SU4949800 priority Critical patent/RU2017408C1/en
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Publication of RU2017408C1 publication Critical patent/RU2017408C1/en
Priority to MD95-0028A priority patent/MD315C2/en

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Abstract

FIELD: agriculture. SUBSTANCE: estimation of plant salt-resistance is carried out by measure of electric resistance of plant leaves under effect of salt stress and under optimal growth conditions. Salt-resistance is evaluated on the basis of electric resistance value change. The higher salt-resistance of plant the less electric resistance change. EFFECT: improved method of salt-resistance estimation. 1 tbl

Description

Изобретение относится к физиологии растений, а именно к способам оценки устойчивости растений к засолению почвы, и может быть применено в растениеводстве и селекции при отборе солеустойчивых сортов. The invention relates to plant physiology, and in particular to methods for assessing the resistance of plants to soil salinization, and can be used in crop production and selection during the selection of salt tolerant varieties.

К аналогам предлагаемого способа можно отнести способы, когда при выращивании растений на засоленных субстратах по всхожести, росту проростков судят о солеустойчивости растений. При этом нельзя оценить как изменяется это свойство в онтогенезе, тогда как известно, что в онтогенезе устойчивость к любым стрессам изменяется, что существенно снижает достоверность оценки. Analogues of the proposed method include methods when, when growing plants on saline substrates by germination, the growth of seedlings is judged on the salt tolerance of the plants. It is impossible to assess how this property changes in ontogenesis, while it is known that in ontogenesis, resistance to any stress changes, which significantly reduces the reliability of the assessment.

Известен способ, по которому о солеустойчивости судят по увеличению электрической емкости корней риса, предварительно обработанных солями. Этот способ применим только к культуре риса, способ трудоемок, так как определяется электрическая емкость извлеченных из почвы корней, предварительно отработанных солями растений, нарушается целостность растений (прототип). A known method according to which salt tolerance is judged by increasing the electric capacity of the roots of rice, pre-treated with salts. This method is applicable only to rice culture, the method is time-consuming, since the electric capacity of the roots extracted from the soil previously worked out by the salts of the plants is determined, the integrity of the plants is violated (prototype).

Целью данного изобретения является повышение достоверности оценки, снижение трудоемкости и ускорение определения солеустойчивости растений. The aim of this invention is to increase the reliability of the assessment, reducing the complexity and accelerating the determination of salt tolerance of plants.

Цель изобретения достигается путем измерения электрического сопротивления листьев без нарушения целостности растений, произрастающих на засоленном субстрате (на полевых участках, где почва засолена или в вегетационных опытах, где создается засоленность субстрата соответствующими солями - хлоридами, сульфатами и др.). Определяют степень изменения электрического сопротивления ткани листьев (ЭСТЛ) в результате солевого воздействия. Чем выше степень воздействия засоления на растение, тем больше изменяется ЭСТЛ в сторону его увеличения. И наоборот, чем устойчивее растение к действию засоления, тем меньше изменяется ЭСТЛ при воздействии засоления. The purpose of the invention is achieved by measuring the electrical resistance of leaves without violating the integrity of plants growing on a saline substrate (in field areas where the soil is saline or in growing experiments where salinity of the substrate is created by the corresponding salts - chlorides, sulfates, etc.). The degree of change in the electrical resistance of leaf tissue (ESTL) as a result of salt exposure is determined. The higher the degree of influence of salinization on a plant, the more the ECTL changes in the direction of its increase. Conversely, the more resistant the plant is to the action of salinization, the less the ECTL changes under the influence of salinization.

П р и м е р. Измеряли ЭСТЛ растений, предпосевно закаленных по методу Генкеля и растений, предпосевно незакаленных после воздействия на них почвенного засоления 0,3% NaCl, что соответствует средней засоленности почвы (таблица). Растения предпосевно закаленные, меньше изменяли ЭСТЛ при воздействии почвенного засоления, чем растений, не закаленные к засолению, т.е. проявили себя как более солеустойчивые. PRI me R. The ESTL of plants presowed hardened by the method of Genkel and plants presowed non-hardened after exposure to soil salinity of 0.3% NaCl was measured, which corresponds to the average salinity of the soil (table). Plants are pre-sowed hardened; they have less changed ECTL under the influence of soil salinization than plants not hardened to salinization, i.e. proved to be more salt tolerant.

Claims (1)

СПОСОБ ОЦЕНКИ СОЛЕУСТОЙЧИВОСТИ РАСТЕНИЙ, включающий измерение биофизического показателя, отличающийся тем, что в качестве биофизического показателя измеряют электрическое сопротивление листьев в период воздействия солевого стресса на растение и об устойчивости к засолению судят по изменению этого показателя в сравнении с уровнем электрического сопротивления листьев растений, находящихся в оптимальных условиях роста, при этом чем меньше изменяется сопротивление, тем выше солеустойчивость растений. METHOD FOR ASSESSING THE SALT-RESISTANCE OF PLANTS, including the measurement of a biophysical indicator, characterized in that the electrical resistance of the leaves during the period of exposure to salt stress on the plant is measured as a biophysical indicator, and salinity resistance is judged by a change in this indicator compared to the level of electrical resistance of the leaves of plants in optimal growth conditions, the less the resistance changes, the higher the salt tolerance of plants.
SU4949800 1991-06-25 1991-06-25 Method of estimation of plant salt-resistance RU2017408C1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SU4949800 RU2017408C1 (en) 1991-06-25 1991-06-25 Method of estimation of plant salt-resistance
MD95-0028A MD315C2 (en) 1991-06-25 1994-12-23 Estimation method of the plants resistance to salt

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Application Number Priority Date Filing Date Title
SU4949800 RU2017408C1 (en) 1991-06-25 1991-06-25 Method of estimation of plant salt-resistance

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RU2017408C1 true RU2017408C1 (en) 1994-08-15

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111855920A (en) * 2020-08-05 2020-10-30 中国农业科学院棉花研究所 Method for identifying alkali stress degree of cotton

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Авторское свидетельство СССР N 940697, кл. A 01G 7/00, 1980. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111855920A (en) * 2020-08-05 2020-10-30 中国农业科学院棉花研究所 Method for identifying alkali stress degree of cotton

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