RU2017144777A - METHOD OF MEASUREMENT OF CATALYTIC ACTIVITY (CATALYTIC CONCENTRATION) ENZYMES USING THE METHODS OF MASS SPECTROMETRY AND SPECTROPHOTOMETRY WITH PRELIMINARY IDENTIFICATION OF PURPOSE ANALYTEMA ACTIVITY CODE INTRODUCTOR ACTIVITY CURRENT METHOD - Google Patents

METHOD OF MEASUREMENT OF CATALYTIC ACTIVITY (CATALYTIC CONCENTRATION) ENZYMES USING THE METHODS OF MASS SPECTROMETRY AND SPECTROPHOTOMETRY WITH PRELIMINARY IDENTIFICATION OF PURPOSE ANALYTEMA ACTIVITY CODE INTRODUCTOR ACTIVITY CURRENT METHOD Download PDF

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RU2017144777A
RU2017144777A RU2017144777A RU2017144777A RU2017144777A RU 2017144777 A RU2017144777 A RU 2017144777A RU 2017144777 A RU2017144777 A RU 2017144777A RU 2017144777 A RU2017144777 A RU 2017144777A RU 2017144777 A RU2017144777 A RU 2017144777A
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Russia
Prior art keywords
activity
catalytic
enzymes
measurement
analytema
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RU2017144777A
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Russian (ru)
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RU2694964C2 (en
RU2017144777A3 (en
Inventor
Елена Валериевна Кулябина
Юрий Алексеевич Кудеяров
Александр Анатольевич Кирилюк
Ольга Николаевна Мелкова
Екатерина Андреевна Гуськова
Original Assignee
ФЕДЕРАЛЬНОЕ АГЕНТСТВО ПО ТЕХНИЧЕСКОМУ РЕГУЛИРОВАНИЮ И МЕТРОЛОГИИ (Росстандарт)
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Priority to RU2017144777A priority Critical patent/RU2694964C2/en
Publication of RU2017144777A publication Critical patent/RU2017144777A/en
Publication of RU2017144777A3 publication Critical patent/RU2017144777A3/ru
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Claims (1)

Способ измерения каталитической концентрации ферментов путем спектрофотометрического измерения оптической плотности продукта ферментативной реакции, отличающийся применением тандемного квадрупольного масс-спектрометра с времяпролетным детектором и высокоэффективного жидкостного хроматографа со спектрофотометрическим детектором, обеспечивающими идентификацию аналита и возможность измерения каталитической активности априорно неизвестного фермента.A method for measuring the catalytic concentration of enzymes by spectrophotometric measurement of the optical density of an enzymatic reaction product, characterized by the use of a tandem quadrupole mass spectrometer with a time-of-flight detector and a high-performance liquid chromatograph with a spectrophotometric detector, providing identification of the analyte and the ability to measure the catalytic activity of a priori unknown enzyme.
RU2017144777A 2017-12-19 2017-12-19 Method of measuring catalytic activity (catalytic concentration) of enzymes using methods of mass spectrometry and spectrophotometry with preliminary identification of target analyte and determination of purity of substances RU2694964C2 (en)

Priority Applications (1)

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RU2017144777A RU2694964C2 (en) 2017-12-19 2017-12-19 Method of measuring catalytic activity (catalytic concentration) of enzymes using methods of mass spectrometry and spectrophotometry with preliminary identification of target analyte and determination of purity of substances

Applications Claiming Priority (1)

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RU2017144777A RU2694964C2 (en) 2017-12-19 2017-12-19 Method of measuring catalytic activity (catalytic concentration) of enzymes using methods of mass spectrometry and spectrophotometry with preliminary identification of target analyte and determination of purity of substances

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RU2017144777A true RU2017144777A (en) 2019-06-20
RU2017144777A3 RU2017144777A3 (en) 2019-06-20
RU2694964C2 RU2694964C2 (en) 2019-07-19

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11396499B2 (en) 2018-12-12 2022-07-26 University Of Washington Lysosomal acid lipase assay

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6649351B2 (en) * 1999-04-30 2003-11-18 Aclara Biosciences, Inc. Methods for detecting a plurality of analytes by mass spectrometry
EP1508806A1 (en) * 2003-08-16 2005-02-23 Aventis Pharma Deutschland GmbH A method for the identification of IRS protein kinase inhibitors or agonists
US8663942B2 (en) * 2009-04-27 2014-03-04 Merz Pharma Gmbh & Co. Kgaa Means and methods for the determination of the amount of neurotoxin polypeptide and of its catalytic and proteolytic activities
RU2518249C1 (en) * 2012-10-16 2014-06-10 Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) Method of determining nonspecific resistance of pathogenic microorganisms to antibiotics based on measuring catalytic activity of phosphodiesterases cleaving cyclic diguanosine monophosphate
WO2016189382A1 (en) * 2015-05-22 2016-12-01 Institut Pasteur Peptides, antibodies, and methods for detection of botulinum neuritoxin a subtypes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11396499B2 (en) 2018-12-12 2022-07-26 University Of Washington Lysosomal acid lipase assay

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RU2694964C2 (en) 2019-07-19
RU2017144777A3 (en) 2019-06-20

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