RU2017128106A - The method of freeze drying of attenuated bacteria B. pertussis, attenuated bacteria B. pertussis, strain of attenuated bacteria B. Pertussis, vaccine, lyophilized vaccine preparation - Google Patents

The method of freeze drying of attenuated bacteria B. pertussis, attenuated bacteria B. pertussis, strain of attenuated bacteria B. Pertussis, vaccine, lyophilized vaccine preparation Download PDF

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RU2017128106A
RU2017128106A RU2017128106A RU2017128106A RU2017128106A RU 2017128106 A RU2017128106 A RU 2017128106A RU 2017128106 A RU2017128106 A RU 2017128106A RU 2017128106 A RU2017128106 A RU 2017128106A RU 2017128106 A RU2017128106 A RU 2017128106A
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pertussis
bacteria
attenuated
attenuated bacteria
vaccine
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RU2017128106A
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RU2709657C2 (en
RU2017128106A3 (en
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Геннадий Иванович Каратаев
Людмила Николаевна Синяшина
Евгений Григорьевич Сёмин
Алиса Юрьевна Медкова
Александр Леонидович Гинцбург
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федеральное государственное бюджетное учреждение "Федеральный научно-исследовательский центр эпидемиологии и микробиологии имени почетного академика Н.Ф. Гамалеи" Министерства здравоохранения Российской Федерации (ФГБУ "ФНИЦЭМ им. Н.Ф. Гамалеи" Минздрава России)
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Priority to RU2017128106A priority Critical patent/RU2709657C2/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/099—Bordetella
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicinal Preparation (AREA)

Claims (5)

1. Аттенуированные бактерии Bordetella pertussis, содержащие мутации в регуляторной и кодирующей областях оперона ptx и кодирующей области гена dnt.1. Attenuated Bordetella pertussis bacteria containing mutations in the regulatory and coding regions of the ptx operon and the coding region of the dnt gene. 2. Штамм аттенуированных бактерий Bordetella pertussis, содержащий мутации в регуляторной и кодирующей областях оперона ptx и кодирующей области гена dnt, депонированный в «Государственной коллекция бактерий II-IV группы патогенности» ФГБУ «ФНИЦЭМ им. Н.Ф. Гамалеи» (в музей лаборатория Генетики бактерий) Минздрава России под №171/332 от 03.03.2014 г. для использования в качестве вакцины.2. The strain of attenuated bacteria Bordetella pertussis containing mutations in the regulatory and coding regions of the ptx operon and the coding region of the dnt gene, deposited in the "State collection of bacteria of the II-IV pathogenicity group" FSBI "FNITsEM im. N.F. Gamalei ”(to the museum, the Laboratory of Genetics of Bacteria) of the Ministry of Health of Russia under No. 171/323 of 03.03.2014 for use as a vaccine. 3. Вакцина против возбудителя коклюша, содержащая аттенуированные бактерии B.pertussis по п. 1.3. A vaccine against pertussis pathogen containing attenuated B.pertussis bacteria according to claim 1. 4. Способ лиофилизации аттенуированных бактерий Bordetella pertussis, предусматривающий выращивание бактерий на твердой среде, суспендирование в защитных средах, последующее замораживание и высушивание, причем в качестве защитных сред используют сахарозо-желатинозную среду, состоящей из сахарозы 10% + желатин 1%, и среду, содержащую декстран 5% + желатин 1% + сахарозу 3%, и желатин 1% + декстран 10%; и замораживание культуры осуществляют при -45°С в течение 15 часов, с последующим высушиванием от одного до двух часов при температуре от -30 до +30°С.4. A method for lyophilization of attenuated Bordetella pertussis bacteria, which involves growing bacteria on a solid medium, suspending in protective media, subsequent freezing and drying, using sucrose-gelatinous medium consisting of 10% sucrose + 1% gelatin and protective medium as protective media containing dextran 5% + gelatin 1% + sucrose 3%, and gelatin 1% + dextran 10%; and freezing the culture is carried out at -45 ° C for 15 hours, followed by drying from one to two hours at a temperature of from -30 to + 30 ° C. 5. Лиофилизированный вакцинный препарат аттенуированных бактерий Bordetella pertussis по п. 1.5. Lyophilized vaccine preparation of attenuated Bordetella pertussis bacteria according to claim 1.
RU2017128106A 2017-08-07 2017-08-07 Method for lyophilic drying of attenuated bacteria b.pertussis, attenuated bacteria b. pertussis, strain of attenuated b. pertussis bacteria, vaccine, lyophilized vaccine preparation RU2709657C2 (en)

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RU2017128106A RU2709657C2 (en) 2017-08-07 2017-08-07 Method for lyophilic drying of attenuated bacteria b.pertussis, attenuated bacteria b. pertussis, strain of attenuated b. pertussis bacteria, vaccine, lyophilized vaccine preparation

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RU2017128106A RU2709657C2 (en) 2017-08-07 2017-08-07 Method for lyophilic drying of attenuated bacteria b.pertussis, attenuated bacteria b. pertussis, strain of attenuated b. pertussis bacteria, vaccine, lyophilized vaccine preparation

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RU2017128106A true RU2017128106A (en) 2019-02-08
RU2017128106A3 RU2017128106A3 (en) 2019-02-22
RU2709657C2 RU2709657C2 (en) 2019-12-19

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WO2022036298A1 (en) * 2020-08-14 2022-02-17 Iliad Biotechnologies, Llc Lyophilized live bordetella vaccines

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RU2017128106A3 (en) 2019-02-22

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