RU2016144507A - A method of obtaining a hybrid protein consisting of a recombinant protein of the interferon gamma analog conjugated to an oligosaccharide - Google Patents

A method of obtaining a hybrid protein consisting of a recombinant protein of the interferon gamma analog conjugated to an oligosaccharide Download PDF

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Publication number
RU2016144507A
RU2016144507A RU2016144507A RU2016144507A RU2016144507A RU 2016144507 A RU2016144507 A RU 2016144507A RU 2016144507 A RU2016144507 A RU 2016144507A RU 2016144507 A RU2016144507 A RU 2016144507A RU 2016144507 A RU2016144507 A RU 2016144507A
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RU
Russia
Prior art keywords
oligosaccharide
interferon gamma
obtaining
recombinant protein
analog conjugated
Prior art date
Application number
RU2016144507A
Other languages
Russian (ru)
Other versions
RU2656140C2 (en
RU2016144507A3 (en
Inventor
Александр Игоревич Мартынов
Михаил Николаевич Санков
Вагиф Али оглы Гасанов
Александр Федорович Шевалье
Александр Алексеевич Власов
Игорь Петрович Шиловский
Original Assignee
Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России filed Critical Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России
Priority to RU2016144507A priority Critical patent/RU2656140C2/en
Publication of RU2016144507A publication Critical patent/RU2016144507A/en
Publication of RU2016144507A3 publication Critical patent/RU2016144507A3/ru
Application granted granted Critical
Publication of RU2656140C2 publication Critical patent/RU2656140C2/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/21—Interferons [IFN]
    • A61K38/217—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma

Claims (1)

Способ получения гибридного белка, обладающего противовирусной активностью, включающий конъюгацию рекомбинантного аналога интерферона гамма, представленного последовательностью SEQ ID NO: 1 с полимером олигосахаридной природы D3000-7(Sa-Sp)-(Sp-M), где D3000 - диальдегид декстран, (Sa-Sp)-Neu5Ac-Lactose-N(CH)-CH(2)-CH(2)-NH(2), (Sp-M)-Малеимид-СН(2)-NH(2), очистку полученного конъюгированного рекомбинантного белка аналога интерферона гамма от не конъюгированных компонентов методами HPLC с гельфильтрационным сорбентом Sephacryl S400 с выходом конечного продукта, содержащим примеси не более 1,2% от общей массы продукта.A method of producing a hybrid protein with antiviral activity, comprising conjugating a recombinant gamma interferon analogue represented by the sequence SEQ ID NO: 1 with an oligosaccharide polymer of the nature D3000-7 (Sa-Sp) - (Sp-M), where D3000 is dextran dialdehyde, (Sa -Sp) -Neu5Ac-Lactose-N (CH) -CH (2) -CH (2) -NH (2), (Sp-M) -Maleimide-CH (2) -NH (2), purification of the obtained conjugated recombinant protein of the interferon gamma analogue from non-conjugated components by HPLC methods with Sephacryl S400 gel filtration sorbent with the release of the final product containing impurities olee 1.2% on total product weight.
RU2016144507A 2016-11-14 2016-11-14 Method for obtaining a hybrid protein containing a fused protein analogue of an interferon gamma conjugated with oligosaccharide RU2656140C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2016144507A RU2656140C2 (en) 2016-11-14 2016-11-14 Method for obtaining a hybrid protein containing a fused protein analogue of an interferon gamma conjugated with oligosaccharide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2016144507A RU2656140C2 (en) 2016-11-14 2016-11-14 Method for obtaining a hybrid protein containing a fused protein analogue of an interferon gamma conjugated with oligosaccharide

Publications (3)

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RU2016144507A true RU2016144507A (en) 2018-05-14
RU2016144507A3 RU2016144507A3 (en) 2018-05-14
RU2656140C2 RU2656140C2 (en) 2018-05-31

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RU2016144507A RU2656140C2 (en) 2016-11-14 2016-11-14 Method for obtaining a hybrid protein containing a fused protein analogue of an interferon gamma conjugated with oligosaccharide

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2753191C2 (en) * 2019-09-26 2021-08-12 Федеральное государственное бюджетное учреждение науки Институт Биологии Развития им. Н.К. Кольцова Российской Академии Наук New recombinant growth hormone analogue with prolonged activity

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2296130C2 (en) * 2001-04-06 2007-03-27 Максиджен Холдингз Лтд. Variants of gamma-interferon polypeptide
US20050176108A1 (en) * 2003-03-13 2005-08-11 Young-Min Kim Physiologically active polypeptide conjugate having prolonged in vivo half-life
TWI376234B (en) * 2005-02-01 2012-11-11 Msd Oss Bv Conjugates of a polypeptide and an oligosaccharide
WO2010146362A2 (en) * 2009-06-16 2010-12-23 The University Of Bath Materials and methods relating to glycosylation

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Publication number Publication date
RU2656140C2 (en) 2018-05-31
RU2016144507A3 (en) 2018-05-14

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