RU2016129359A - Method for isolating DNA of clinical isolates of Mycobacterium tuberculosis from lung tissue - Google Patents

Method for isolating DNA of clinical isolates of Mycobacterium tuberculosis from lung tissue Download PDF

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Publication number
RU2016129359A
RU2016129359A RU2016129359A RU2016129359A RU2016129359A RU 2016129359 A RU2016129359 A RU 2016129359A RU 2016129359 A RU2016129359 A RU 2016129359A RU 2016129359 A RU2016129359 A RU 2016129359A RU 2016129359 A RU2016129359 A RU 2016129359A
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RU
Russia
Prior art keywords
lung tissue
mycobacterium tuberculosis
clinical isolates
isolating dna
sample
Prior art date
Application number
RU2016129359A
Other languages
Russian (ru)
Other versions
RU2659197C2 (en
Inventor
Майя Александровна Дымова
Ольга Ивановна Альховик
Андрей Георгиевич Чередниченко
Любовь Станиславовна Евдокимова
Денис Владимирович Краснов
Татьяна Игоревна Петренко
Original Assignee
ФГБУ "Новосибирский научно-исследовательский институт туберкулеза" Министерства здравоохранения Российской Федерации
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Priority to RU2016129359A priority Critical patent/RU2659197C2/en
Publication of RU2016129359A publication Critical patent/RU2016129359A/en
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Publication of RU2659197C2 publication Critical patent/RU2659197C2/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

Claims (2)

1. Способ выделения ДНК клинических изолятов Mycobacterium tuberculosis из ткани легкого путем одновременного действия химических и физических факторов, отличающийся тем, что к образцу, находящемуся в специальной пробирке, добавляют деконтаминирующий, лизирующий буфер следующего состава: 5 М GuSCN, 100 мМ TRIS HCl рН 8.0, 10 мМ EDTA рН 8.0, 100 мМ NaCl, 0.5% SDS;1. A method for isolating DNA of clinical isolates of Mycobacterium tuberculosis from lung tissue by the simultaneous action of chemical and physical factors, characterized in that a decontamination, lysis buffer of the following composition is added to the sample in a special tube: 5 M GuSCN, 100 mM TRIS HCl pH 8.0 10 mM EDTA pH 8.0, 100 mM NaCl, 0.5% SDS; 2. Способ по п. 1, отличающийся тем, что деконтаминирующий, лизирующий раствор добавляют непосредственно в пробирку, содержащую образец ткани легкого, и керамические, стеклянные шарики.2. The method according to p. 1, characterized in that the decontaminating, lysing solution is added directly to a test tube containing a sample of lung tissue, and ceramic, glass balls.
RU2016129359A 2016-07-18 2016-07-18 Method for isolation of clinical isolates of mycobacterium tuberculosis from lung tissue RU2659197C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2016129359A RU2659197C2 (en) 2016-07-18 2016-07-18 Method for isolation of clinical isolates of mycobacterium tuberculosis from lung tissue

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2016129359A RU2659197C2 (en) 2016-07-18 2016-07-18 Method for isolation of clinical isolates of mycobacterium tuberculosis from lung tissue

Publications (2)

Publication Number Publication Date
RU2016129359A true RU2016129359A (en) 2018-01-23
RU2659197C2 RU2659197C2 (en) 2018-06-28

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU2016129359A RU2659197C2 (en) 2016-07-18 2016-07-18 Method for isolation of clinical isolates of mycobacterium tuberculosis from lung tissue

Country Status (1)

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RU (1) RU2659197C2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110964842A (en) * 2019-12-31 2020-04-07 广州金域医学检验集团股份有限公司 Sample processing reagent and kit for detecting mycobacterium tuberculosis nucleic acid and nucleic acid amplification method of mycobacterium tuberculosis

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5130423A (en) * 1990-07-13 1992-07-14 Microprobe Corporation Non-corrosive compositions and methods useful for the extraction of nucleic acids
US5712095A (en) * 1994-06-16 1998-01-27 Becton Dickinson And Company Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
CA2609636A1 (en) * 2005-02-17 2006-08-24 Istituto Nazionale Per Le Malattie Infettive Irccs Lazzaro Spallanzani Compositions and methods for detecting pathogen specific nucleic acids in urine
US9328326B2 (en) * 2006-08-10 2016-05-03 Merck Patent Gmbh Method for isolating cells

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110964842A (en) * 2019-12-31 2020-04-07 广州金域医学检验集团股份有限公司 Sample processing reagent and kit for detecting mycobacterium tuberculosis nucleic acid and nucleic acid amplification method of mycobacterium tuberculosis
CN110964842B (en) * 2019-12-31 2023-11-03 广州金域医学检验集团股份有限公司 Sample processing reagent for detecting mycobacterium tuberculosis nucleic acid, kit and mycobacterium tuberculosis nucleic acid amplification method

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Publication number Publication date
RU2659197C2 (en) 2018-06-28

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FZ9A Application not withdrawn (correction of the notice of withdrawal)

Effective date: 20180403