RU2016115461A - Recombinant cassette containing EP153R and EP364R genes of Congo strain (KK-262) of African swine fever virus and recombinant African swine fever virus ΔCongoCD2v strain - Google Patents

Recombinant cassette containing EP153R and EP364R genes of Congo strain (KK-262) of African swine fever virus and recombinant African swine fever virus ΔCongoCD2v strain Download PDF

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RU2016115461A
RU2016115461A RU2016115461A RU2016115461A RU2016115461A RU 2016115461 A RU2016115461 A RU 2016115461A RU 2016115461 A RU2016115461 A RU 2016115461A RU 2016115461 A RU2016115461 A RU 2016115461A RU 2016115461 A RU2016115461 A RU 2016115461A
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recombinant
swine fever
fever virus
african swine
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RU2016115461A
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RU2654586C2 (en
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Галина Сергеевна Бурмакина
Юрий Петрович Моргунов
Илья Андреевич Титов
Ксения Александровна Мима
Денис Владимирович Колбасов
Александр Сергеевич Малоголовкин
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Государственное научное учреждение Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии Российской академии сельскохозяйственных наук
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof

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Claims (2)

1. Рекомбинационная кассета для получения рекомбинантного штамма ΔCongoCD2v представляет собой плазмиду на основе вектора pUC19, содержащую фрагменты генов EP153R и EP364R штамма КК-262 вируса АЧС, а также репортерный ген EGFP под контролем р72 промотора.1. The recombinant cassette for producing the recombinant strain ΔCongoCD2v is a plasmid based on the pUC19 vector containing fragments of the EP153R and EP364R genes of ASF virus strain KK-262, as well as the EGFP reporter gene under the control of the p72 promoter. 2. Рекомбинантный штамм ΔCongoCD2v вируса африканской чумы свиней с делецией гена EP402R (CD2v), содержащий репортерный ген EGFP под контролем р72 промотора, для изучения роли белка CD2v вируса АЧС в репродукции вируса в культурах клеток, в восприимчивых животных, а также его функций в модуляции иммунного ответа.2. Recombinant strain of African swine fever virus ΔCongoCD2v with deletion of the EP402R gene (CD2v), containing the EGFP reporter gene under the control of the p72 promoter, to study the role of ASF virus CD2v protein in virus reproduction in cell cultures, in susceptible animals, and its modulation functions immune response.
RU2016115461A 2016-04-20 2016-04-20 Recombinational cartridge containing the ep153r and ep364r genes of the congo (kk-262) strain of the african swine fever virus and recombinant δcongocd2v strain of the african swine fever virus RU2654586C2 (en)

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RU2016115461A RU2654586C2 (en) 2016-04-20 2016-04-20 Recombinational cartridge containing the ep153r and ep364r genes of the congo (kk-262) strain of the african swine fever virus and recombinant δcongocd2v strain of the african swine fever virus

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111471089A (en) * 2019-01-24 2020-07-31 浙江海隆生物科技有限公司 Recombinant African swine fever virus CD2V subunit protein and preparation method and application thereof
CN112472801A (en) * 2020-12-22 2021-03-12 华南农业大学 DNA vaccine and subunit vaccine of African swine fever p30, p54, p72 and B602L, and preparation method and application thereof
CN113122655A (en) * 2019-12-30 2021-07-16 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） TaqMan fluorescent quantitative PCR (polymerase chain reaction) detection method for African swine fever virus EP402R gene
CN113150079A (en) * 2021-01-21 2021-07-23 中国检验检疫科学研究院 Eukaryotic expression African swine fever virus p72 antigen and application thereof
CN114375199A (en) * 2019-07-29 2022-04-19 皮尔布莱特研究所 African swine fever vaccine
CN114761039A (en) * 2019-09-16 2022-07-15 陈大路 Methods of blocking ASFV infection by interfering with cellular receptors

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108504687A (en) * 2018-03-28 2018-09-07 扬州大学 A kind of expression African swine fever virus EP402R gene recombinant adenovirus vectors, construction method and recombined adhenovirus preparation method
CN111172321B (en) * 2020-01-02 2021-04-23 中国检验检疫科学研究院 Fluorescent PCR detection kit for identifying African swine fever infection and immunity

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2452511C1 (en) * 2010-11-16 2012-06-10 Государственное научное учреждение Всероссийский научно-исследовательский институт ветеринарной вирусологии и микробиологии Attenuated strain of serotype 2 african swine fever virus for developing diagnostic and vaccine preparations
EP2649201A4 (en) * 2010-12-10 2014-10-01 Univ Brandeis Compositions and methods for the detection and analysis of african swine fever virus

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111471089A (en) * 2019-01-24 2020-07-31 浙江海隆生物科技有限公司 Recombinant African swine fever virus CD2V subunit protein and preparation method and application thereof
CN111471089B (en) * 2019-01-24 2023-11-24 浙江海隆生物科技有限公司 Recombinant African swine fever virus CD2V subunit protein and preparation method and application thereof
CN114375199A (en) * 2019-07-29 2022-04-19 皮尔布莱特研究所 African swine fever vaccine
CN114761039A (en) * 2019-09-16 2022-07-15 陈大路 Methods of blocking ASFV infection by interfering with cellular receptors
CN113122655A (en) * 2019-12-30 2021-07-16 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） TaqMan fluorescent quantitative PCR (polymerase chain reaction) detection method for African swine fever virus EP402R gene
CN112472801A (en) * 2020-12-22 2021-03-12 华南农业大学 DNA vaccine and subunit vaccine of African swine fever p30, p54, p72 and B602L, and preparation method and application thereof
CN113150079A (en) * 2021-01-21 2021-07-23 中国检验检疫科学研究院 Eukaryotic expression African swine fever virus p72 antigen and application thereof

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