RU2014123868A - METHOD FOR PRODUCING THROMBIN CONCENTRATE - Google Patents
METHOD FOR PRODUCING THROMBIN CONCENTRATE Download PDFInfo
- Publication number
- RU2014123868A RU2014123868A RU2014123868/15A RU2014123868A RU2014123868A RU 2014123868 A RU2014123868 A RU 2014123868A RU 2014123868/15 A RU2014123868/15 A RU 2014123868/15A RU 2014123868 A RU2014123868 A RU 2014123868A RU 2014123868 A RU2014123868 A RU 2014123868A
- Authority
- RU
- Russia
- Prior art keywords
- prothrombin
- cacl
- thrombin
- solution
- isolation
- Prior art date
Links
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/16—Blood plasma; Blood serum
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6429—Thrombin (3.4.21.5)
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Virology (AREA)
- Veterinary Medicine (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Developmental Biology & Embryology (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Способ выделения очищенного концентрата тромбина, избавленного от вирусов, характеризующегося большим выходом, чистотой и высокой стабильностью, заключающийся в криофракционировании исходного сырья, представленного свежезамороженной плазмой донорской крови человека, выделении протромбинового комплекса на анионообменном сорбенте DEAE Sepharose Fast Flow, активации протромбина раствором CaClв составе протромбинового комплекса с получением тромбина, вирусной инактивации сольвент - детергентным методом и очистке полученного тромбина на катионообменном сорбенте Fractogel EMD-SO, отличающийся тем, что количество раствора CaCl, выступающего в качестве активатора протромбина, рассчитывается с учетом взаимодействия CaClс цитратом натрия, который входит в состав буфера, используемого при выделении протромбинового комплекса на анионобменном сорбенте, так, чтобы концентрация свободных ионов Сав растворе на стадии активации составляла от 2 до 20 ммоль/л.A method for isolating a purified thrombin concentrate, freed from viruses, characterized by a high yield, purity, and high stability, which consists in cryofractionation of the feedstock represented by freshly frozen human blood donated plasma, isolation of the prothrombin complex on the DEAE Sepharose Fast Flow anion-exchange sorbent, prothrombin activation with CaCl solution in the prothrombin complex with obtaining thrombin, viral inactivation of the solvent - detergent method and purification of the obtained thrombin on cation Fractogel EMD-SO belt sorbent, characterized in that the amount of CaCl solution acting as a prothrombin activator is calculated taking into account the interaction of CaCl with sodium citrate, which is part of the buffer used in the isolation of the prothrombin complex on the anion exchange sorbent, so that the concentration of free ions Cav solution at the stage of activation ranged from 2 to 20 mmol / L.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2014123868/15A RU2583931C2 (en) | 2014-06-11 | 2014-06-11 | Method of producing thrombin concentrate |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2014123868/15A RU2583931C2 (en) | 2014-06-11 | 2014-06-11 | Method of producing thrombin concentrate |
Publications (2)
Publication Number | Publication Date |
---|---|
RU2014123868A true RU2014123868A (en) | 2015-12-20 |
RU2583931C2 RU2583931C2 (en) | 2016-05-10 |
Family
ID=54871142
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2014123868/15A RU2583931C2 (en) | 2014-06-11 | 2014-06-11 | Method of producing thrombin concentrate |
Country Status (1)
Country | Link |
---|---|
RU (1) | RU2583931C2 (en) |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5981254A (en) * | 1997-10-30 | 1999-11-09 | Haemacure Corporation | Process for producing thrombin from plasma |
DE10012732A1 (en) * | 2000-03-18 | 2001-09-20 | Aventis Behring Gmbh | Thrombin composition, for use as hemostatic or as a component of fibrin glues, comprises non-covalently bonded inhibitor for stabilization |
RU2457248C2 (en) * | 2010-06-09 | 2012-07-27 | Общество с ограниченной ответственностью фирма "Технология-Стандарт" | Method for commercial production of thrombin |
-
2014
- 2014-06-11 RU RU2014123868/15A patent/RU2583931C2/en active
Also Published As
Publication number | Publication date |
---|---|
RU2583931C2 (en) | 2016-05-10 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RH4A | Copy of patent granted that was duplicated for the russian federation |
Effective date: 20200901 |