RU2013143366A - METHOD OF CARRYING OUT MEMBRANE IMMUNO ANALYSIS OF ANTIGENS AND ANTIBODIES USING FLUORESCENT MARKERS - Google Patents

METHOD OF CARRYING OUT MEMBRANE IMMUNO ANALYSIS OF ANTIGENS AND ANTIBODIES USING FLUORESCENT MARKERS Download PDF

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RU2013143366A
RU2013143366A RU2013143366/15A RU2013143366A RU2013143366A RU 2013143366 A RU2013143366 A RU 2013143366A RU 2013143366/15 A RU2013143366/15 A RU 2013143366/15A RU 2013143366 A RU2013143366 A RU 2013143366A RU 2013143366 A RU2013143366 A RU 2013143366A
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antigens
antibodies
fluorescence
fluorescent markers
intensity
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RU2013143366/15A
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Russian (ru)
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Надежда Алексеевна Таранова
Анатолий Виталиевич Жердев
Борис Борисович Дзантиев
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Федеральное государственное бюджетное учреждение науки Институт биохимии имени А.Н. Баха РАН Российской академии наук (ИНБИ РАН)
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Priority to RU2013143366/15A priority Critical patent/RU2013143366A/en
Publication of RU2013143366A publication Critical patent/RU2013143366A/en

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  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Методика проведения мембранного иммуноанализа антигенов и антител с использованием флуоресцентных маркеров, основанная на движении реагентов с током жидкости вдоль мембраны и формировании в определенных ее участках специфических иммунных комплексов, детектируемых по флуоресценции маркеров, отличающаяся тем, что при детекции флуоресценции с целью обеспечения предельной чувствительности и расширения рабочего диапазона производится варьирование интенсивности возбуждающего света, и флуоресценция регистрируется как минимум для двух вариантов интенсивности возбуждающего света.The method of conducting membrane immunoassay of antigens and antibodies using fluorescent markers, based on the movement of reagents with fluid flow along the membrane and the formation of specific immune complexes detected in its specific areas, detected by fluorescence markers, characterized in that when detecting fluorescence in order to ensure ultimate sensitivity and expansion the working range, the intensity of the exciting light is varied, and fluorescence is recorded for at least two options for the intensity of exciting light.

Claims (1)

Методика проведения мембранного иммуноанализа антигенов и антител с использованием флуоресцентных маркеров, основанная на движении реагентов с током жидкости вдоль мембраны и формировании в определенных ее участках специфических иммунных комплексов, детектируемых по флуоресценции маркеров, отличающаяся тем, что при детекции флуоресценции с целью обеспечения предельной чувствительности и расширения рабочего диапазона производится варьирование интенсивности возбуждающего света, и флуоресценция регистрируется как минимум для двух вариантов интенсивности возбуждающего света. The method of conducting membrane immunoassay of antigens and antibodies using fluorescent markers, based on the movement of reagents with fluid flow along the membrane and the formation of specific immune complexes detected in its specific areas, detected by fluorescence markers, characterized in that when detecting fluorescence in order to ensure ultimate sensitivity and expansion the working range, the intensity of the exciting light is varied, and fluorescence is recorded for at least two options for the intensity of exciting light.
RU2013143366/15A 2013-09-25 2013-09-25 METHOD OF CARRYING OUT MEMBRANE IMMUNO ANALYSIS OF ANTIGENS AND ANTIBODIES USING FLUORESCENT MARKERS RU2013143366A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2739752C1 (en) * 2019-12-27 2020-12-28 Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук" (ФИЦ Биотехнологии РАН) Method for increasing sensitivity of immunochromatographic serodiagnostic assay using two markers

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2739752C1 (en) * 2019-12-27 2020-12-28 Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук" (ФИЦ Биотехнологии РАН) Method for increasing sensitivity of immunochromatographic serodiagnostic assay using two markers

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