RU2013138105A - METHOD FOR REPLENISHING TEA COTTLER (HEDYSARUM THEINUM KRASNOB.) - Google Patents

METHOD FOR REPLENISHING TEA COTTLER (HEDYSARUM THEINUM KRASNOB.) Download PDF

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RU2013138105A
RU2013138105A RU2013138105/10A RU2013138105A RU2013138105A RU 2013138105 A RU2013138105 A RU 2013138105A RU 2013138105/10 A RU2013138105/10 A RU 2013138105/10A RU 2013138105 A RU2013138105 A RU 2013138105A RU 2013138105 A RU2013138105 A RU 2013138105A
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Prior art keywords
bap
media
krasnob
propagation
tea
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RU2013138105/10A
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Russian (ru)
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RU2547593C2 (en
Inventor
Людмила Ивановна Тихомирова
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Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Алтайский государственный университет"
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Abstract

Способ размножения копеечника чайного (Hedysarum theinum Krasnob.), включающий в себя культуру ткани, отличающийся тем, что используют семена копеечника чайного, которые высаживают на питательные среды Мурасиге-Скуга (MS), несодержащие гормоны, через 20-30 суток развившиеся побеги пересаживают на среды размножения MS, с добавлением 5 мкМ БАП, 2,5 мкМ кинетина, 5 мкМ ГК (гибберелловой кислоты), образовавшиеся микропобеги, делят и пересаживают на среды, содержащие 1,0 БАП+L-глютамин и аденин сульфат 100 мг/л, чередуют среды с высоким и низким содержанием БАП через один пассаж, укореняют побеги на среде MS, дополненной 3 мкМ НУК, адаптируют к нестерильным условиям на гидропонной установке с ¼ минерального состава среды MS.The method of propagation of tea penny (Hedysarum theinum Krasnob.), Which includes a tissue culture, characterized in that they use tea penny seeds, which are planted on Murashige-Skoog (MS) culture media, not containing hormones, after 20-30 days the developed shoots are transplanted onto MS propagation media, with the addition of 5 μM BAP, 2.5 μM kinetin, 5 μM HA (gibberellic acid), the resulting microburns are divided and transplanted onto media containing 1.0 BAP + L-glutamine and adenine sulfate 100 mg / L, alternate environments with high and low BAP content through od N passage rooting shoots on MS medium supplemented with 3 mM NAA, adapted to non-sterile conditions on hydroponic plant with ¼ mineral composition of MS medium.

Claims (1)

Способ размножения копеечника чайного (Hedysarum theinum Krasnob.), включающий в себя культуру ткани, отличающийся тем, что используют семена копеечника чайного, которые высаживают на питательные среды Мурасиге-Скуга (MS), несодержащие гормоны, через 20-30 суток развившиеся побеги пересаживают на среды размножения MS, с добавлением 5 мкМ БАП, 2,5 мкМ кинетина, 5 мкМ ГК (гибберелловой кислоты), образовавшиеся микропобеги, делят и пересаживают на среды, содержащие 1,0 БАП+L-глютамин и аденин сульфат 100 мг/л, чередуют среды с высоким и низким содержанием БАП через один пассаж, укореняют побеги на среде MS, дополненной 3 мкМ НУК, адаптируют к нестерильным условиям на гидропонной установке с ¼ минерального состава среды MS. The method of propagation of tea penny (Hedysarum theinum Krasnob.), Which includes a tissue culture, characterized in that they use tea penny seeds, which are planted on Murashige-Skoog (MS) culture media, not containing hormones, after 20-30 days the developed shoots are transplanted onto MS propagation media, with the addition of 5 μM BAP, 2.5 μM kinetin, 5 μM HA (gibberellic acid), the resulting microburns are divided and transplanted onto media containing 1.0 BAP + L-glutamine and adenine sulfate 100 mg / L, alternate environments with high and low BAP content through od N passage rooting shoots on MS medium supplemented with 3 mM NAA, adapted to non-sterile conditions on hydroponic plant with ¼ mineral composition of MS medium.
RU2013138105/10A 2013-08-14 2013-08-14 Method of reproduction of tea tick trefoil (hedysarum theinum krasnob) RU2547593C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2013138105/10A RU2547593C2 (en) 2013-08-14 2013-08-14 Method of reproduction of tea tick trefoil (hedysarum theinum krasnob)

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Application Number Priority Date Filing Date Title
RU2013138105/10A RU2547593C2 (en) 2013-08-14 2013-08-14 Method of reproduction of tea tick trefoil (hedysarum theinum krasnob)

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RU2013138105A true RU2013138105A (en) 2015-02-20
RU2547593C2 RU2547593C2 (en) 2015-04-10

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107232056B (en) * 2017-06-19 2019-11-01 西北农林科技大学 A kind of method for building up of tea-tree tissue culture rapid propagation system

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2360964C1 (en) * 2008-02-07 2009-07-10 Институт Физиологии растений им. К.А. Тимирязева Российской Академии Наук CULTURE OF ROOT STRAIN Hed th (Hedysarum theinum Krasnob) - PRODUCER OF ISOFLAVONS

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Effective date: 20180815