RU2013135816A - A SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENT-TESTED PROBE FOR A SPECIFIC EXPRESS IDENTIFICATION OF LASS VIRUS RNA BY A REAL-TIME POLYMERASE CHAIN REACTION - Google Patents

A SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENT-TESTED PROBE FOR A SPECIFIC EXPRESS IDENTIFICATION OF LASS VIRUS RNA BY A REAL-TIME POLYMERASE CHAIN REACTION Download PDF

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RU2013135816A
RU2013135816A RU2013135816/10A RU2013135816A RU2013135816A RU 2013135816 A RU2013135816 A RU 2013135816A RU 2013135816/10 A RU2013135816/10 A RU 2013135816/10A RU 2013135816 A RU2013135816 A RU 2013135816A RU 2013135816 A RU2013135816 A RU 2013135816A
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RU
Russia
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real
chain reaction
polymerase chain
oligonucleotide primers
time polymerase
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RU2013135816/10A
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Russian (ru)
Inventor
Владимир Александрович Терновой
Андрей Николаевич Шиков
Александра Олеговна Семенцова
Александр Петрович Агафонов
Александр Николаевич Сергеев
Original Assignee
Федеральное бюджетное учреждение науки "Государственный научный центр вирусологии и биотехнологии "Вектор"" (ФБУН ГНЦ ВБ "Вектор")
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Priority to RU2013135816/10A priority Critical patent/RU2013135816A/en
Publication of RU2013135816A publication Critical patent/RU2013135816A/en

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Abstract

Набор олигонуклеотидных праймеров и флуоресцентномеченого зонда для видоспецифичной экспресс-идентификации РНК вируса Ласса методом полимеразной цепной реакции в реальном времени, включающий последовательности олигонуклеотидов, видоспецифичные для вируса Ласса и имеющие следующую структуру:внешний праймер:5΄→3΄ 5΄ GAGACAAGAGACAARGCTTCACAGTT 3΄внутренний праймер:5΄→3΄ 5΄ TGGAGGATAGGGTTGGTTTGAAT 3΄зонд:ROX- CACTTCATTTTGGTTGACGCTAGATCGCTC-BHQ2 A set of oligonucleotide primers and a fluorescently labeled probe for species-specific rapid identification of Rass Lass virus RNA by real-time polymerase chain reaction, including oligonucleotide sequences species-specific for Lass virus and having the following structure: external primer: 5΄ → 3΄ 5΄ GAGACGTACTCAGACAмерTCAGTAGAC : 5΄ → 3΄ 5΄ TGGAGGATAGGGTTGGTTTGAAT 3΄ probe: ROX- CACTTCATTTTGGTTGACGCTAGATCGCTC-BHQ2

Claims (1)

Набор олигонуклеотидных праймеров и флуоресцентномеченого зонда для видоспецифичной экспресс-идентификации РНК вируса Ласса методом полимеразной цепной реакции в реальном времени, включающий последовательности олигонуклеотидов, видоспецифичные для вируса Ласса и имеющие следующую структуру:A set of oligonucleotide primers and a fluorescently labeled probe for species-specific rapid identification of Lass virus RNA by real-time polymerase chain reaction, including oligonucleotide sequences species-specific for Lass virus and having the following structure: внешний праймер:external primer: 5΄→3΄ 5΄ GAGACAAGAGACAARGCTTCACAGTT 3΄5΄ → 3΄ 5΄ GAGACAAGAGACAARGCTTCACAGTT 3΄ внутренний праймер:internal primer: 5΄→3΄ 5΄ TGGAGGATAGGGTTGGTTTGAAT 3΄5΄ → 3΄ 5΄ TGGAGGATAGGGTTGGTTTGAAT 3΄ зонд:probe: ROX- CACTTCATTTTGGTTGACGCTAGATCGCTC-BHQ2 ROX- CACTTCATTTTGGTTGACGCTAGATCGCTC-BHQ2
RU2013135816/10A 2013-07-30 2013-07-30 A SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENT-TESTED PROBE FOR A SPECIFIC EXPRESS IDENTIFICATION OF LASS VIRUS RNA BY A REAL-TIME POLYMERASE CHAIN REACTION RU2013135816A (en)

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RU2013135816/10A RU2013135816A (en) 2013-07-30 2013-07-30 A SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENT-TESTED PROBE FOR A SPECIFIC EXPRESS IDENTIFICATION OF LASS VIRUS RNA BY A REAL-TIME POLYMERASE CHAIN REACTION

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RU2013135816/10A RU2013135816A (en) 2013-07-30 2013-07-30 A SET OF OLIGONUCLEOTIDE PRIMERS AND A FLUORESCENT-TESTED PROBE FOR A SPECIFIC EXPRESS IDENTIFICATION OF LASS VIRUS RNA BY A REAL-TIME POLYMERASE CHAIN REACTION

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109338016A (en) * 2018-11-06 2019-02-15 南通国际旅行卫生保健门诊部 Lassa virus rapid fluorescence PCR detection kit and its primer combination of probe

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109338016A (en) * 2018-11-06 2019-02-15 南通国际旅行卫生保健门诊部 Lassa virus rapid fluorescence PCR detection kit and its primer combination of probe

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