RU2013125371A - METHOD FOR IDENTIFYING UREAZ-PRODUCING MICRO-ORGANISMS "FAST UREASIC TEST HELICOBACTER TEST" AND DEVICE FOR ITS APPLICATION - Google Patents

METHOD FOR IDENTIFYING UREAZ-PRODUCING MICRO-ORGANISMS "FAST UREASIC TEST HELICOBACTER TEST" AND DEVICE FOR ITS APPLICATION Download PDF

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RU2013125371A
RU2013125371A RU2013125371/15A RU2013125371A RU2013125371A RU 2013125371 A RU2013125371 A RU 2013125371A RU 2013125371/15 A RU2013125371/15 A RU 2013125371/15A RU 2013125371 A RU2013125371 A RU 2013125371A RU 2013125371 A RU2013125371 A RU 2013125371A
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test
urease
reaction medium
urea
indicator
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RU2013125371/15A
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Russian (ru)
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RU2540473C2 (en
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Плисс Михаил Гениевич
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  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Способ выявления продуцирующих уреазу микроорганизмов, заключающийся во внесении в среду, содержащую мочевину, кондиционирующих эту реакционную среду химических веществ и индикатора, реагирующего изменением цвета на сдвиг рН среды вызванный выделением аммиака при разложении мочевины уреазой тестируемых микроорганизмов, и выполненного в виде диагностических дисков из природных или синтететичесих полимеров, отличающийся тем, что, с целью повышения чувствительности метода, реакционная среда гипертонична и содержит вещества органического и неорганического происхождения, вызывающие повреждение клеточной стенки и выход в реакционную среду эндогенной уреазы.A method for detecting urease-producing microorganisms, which consists in introducing chemicals and an indicator in a urea-containing medium that condition this reaction medium and an indicator that responds by changing the color to a pH shift caused by the release of ammonia during the decomposition of urea by urease of the tested microorganisms and made in the form of diagnostic disks from natural or synthetic polymers, characterized in that, in order to increase the sensitivity of the method, the reaction medium is hypertonic and contains organic substances coma and inorganic origin, causing damage to the cell wall and exit into the reaction medium of endogenous urease.

Claims (1)

Способ выявления продуцирующих уреазу микроорганизмов, заключающийся во внесении в среду, содержащую мочевину, кондиционирующих эту реакционную среду химических веществ и индикатора, реагирующего изменением цвета на сдвиг рН среды вызванный выделением аммиака при разложении мочевины уреазой тестируемых микроорганизмов, и выполненного в виде диагностических дисков из природных или синтететичесих полимеров, отличающийся тем, что, с целью повышения чувствительности метода, реакционная среда гипертонична и содержит вещества органического и неорганического происхождения, вызывающие повреждение клеточной стенки и выход в реакционную среду эндогенной уреазы. A method for detecting urease-producing microorganisms, which consists in introducing chemicals and an indicator in a urea-containing medium that condition this reaction medium and an indicator that responds by changing the color to a pH shift caused by the release of ammonia during the decomposition of urea by urease of the tested microorganisms and made in the form of diagnostic disks from natural or synthetic polymers, characterized in that, in order to increase the sensitivity of the method, the reaction medium is hypertonic and contains organic substances coma and inorganic origin, causing damage to the cell wall and exit into the reaction medium of endogenous urease.
RU2013125371/15A 2013-05-28 2013-05-28 Method of detecting urease-producing microorganisms "rapid urease test helicobacter test" and device for its application RU2540473C2 (en)

Priority Applications (1)

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RU2013125371/15A RU2540473C2 (en) 2013-05-28 2013-05-28 Method of detecting urease-producing microorganisms "rapid urease test helicobacter test" and device for its application

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RU2013125371/15A RU2540473C2 (en) 2013-05-28 2013-05-28 Method of detecting urease-producing microorganisms "rapid urease test helicobacter test" and device for its application

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RU2013125371A true RU2013125371A (en) 2014-12-10
RU2540473C2 RU2540473C2 (en) 2015-02-10

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RU2013125371/15A RU2540473C2 (en) 2013-05-28 2013-05-28 Method of detecting urease-producing microorganisms "rapid urease test helicobacter test" and device for its application

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2591622C1 (en) * 2015-06-24 2016-07-20 Михаил Львович Штейнер Method of optimising diagnosis of helicobacteriosis

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2359896T3 (en) * 2007-03-13 2011-05-27 Abs Advanced Biomedical Systems Srl A DIAGNOSTIC METHOD FOR THE DETERMINATION OF HELICOBACTER PYLORI.

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Effective date: 20150529