RU2013123078A - METHOD FOR PRODUCING BIFIDOGENIC FACTOR - Google Patents

METHOD FOR PRODUCING BIFIDOGENIC FACTOR Download PDF

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Publication number
RU2013123078A
RU2013123078A RU2013123078/10A RU2013123078A RU2013123078A RU 2013123078 A RU2013123078 A RU 2013123078A RU 2013123078/10 A RU2013123078/10 A RU 2013123078/10A RU 2013123078 A RU2013123078 A RU 2013123078A RU 2013123078 A RU2013123078 A RU 2013123078A
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RU
Russia
Prior art keywords
producing
bifidogenic factor
sepharose
bifidogenic
factor
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RU2013123078/10A
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Russian (ru)
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RU2553513C2 (en
Inventor
Андрей Сергеевич Сухих
Юлия Викторовна Захарова
Алексей Николаевич Волков
Original Assignee
Андрей Сергеевич Сухих
Юлия Викторовна Захарова
Алексей Николаевич Волков
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Priority to RU2013123078/10A priority Critical patent/RU2553513C2/en
Publication of RU2013123078A publication Critical patent/RU2013123078A/en
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Publication of RU2553513C2 publication Critical patent/RU2553513C2/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Способ получения бифидогенного фактора, отличающийся тем, что в качестве бифидогенного фактора используется дезоксирибонуклеиновая кислота, которая выделяется из накопленной клеточной массы необходимого штамма бифидобактерий в процессе ультразвуковой обработки при частоте 40кГц с последующим отделением супернатанта центрифугированием при 5 тыс. об/мин и хроматографированием с использованием сефарозы (Sepharose) CL-4B.A method of producing a bifidogenic factor, characterized in that deoxyribonucleic acid is used as the bifidogenic factor, which is released from the accumulated cell mass of the required strain of bifidobacteria in the process of ultrasonic treatment at a frequency of 40 kHz, followed by separation of the supernatant by centrifugation at 5 thousand rpm and chromatography using sepharose (Sepharose) CL-4B.

Claims (1)

Способ получения бифидогенного фактора, отличающийся тем, что в качестве бифидогенного фактора используется дезоксирибонуклеиновая кислота, которая выделяется из накопленной клеточной массы необходимого штамма бифидобактерий в процессе ультразвуковой обработки при частоте 40кГц с последующим отделением супернатанта центрифугированием при 5 тыс. об/мин и хроматографированием с использованием сефарозы (Sepharose) CL-4B. A method of producing a bifidogenic factor, characterized in that deoxyribonucleic acid is used as the bifidogenic factor, which is released from the accumulated cell mass of the required strain of bifidobacteria in the process of ultrasonic treatment at a frequency of 40 kHz, followed by separation of the supernatant by centrifugation at 5 thousand rpm and chromatography using sepharose (Sepharose) CL-4B.
RU2013123078/10A 2013-05-20 2013-05-20 Method of obtaining bifidogenic factor RU2553513C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2013123078/10A RU2553513C2 (en) 2013-05-20 2013-05-20 Method of obtaining bifidogenic factor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2013123078/10A RU2553513C2 (en) 2013-05-20 2013-05-20 Method of obtaining bifidogenic factor

Publications (2)

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RU2013123078A true RU2013123078A (en) 2014-11-27
RU2553513C2 RU2553513C2 (en) 2015-06-20

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2738671C1 (en) * 2020-02-26 2020-12-15 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кемеровский медицинский университет" (ФГБОУ ВО КемГМУ) Method for producing and purifying biologically active substance produced by bifidobacteria

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1124033A1 (en) * 1983-02-16 1984-11-15 Предприятие П/Я Г-4740 Method for preparing dna plasmid
RU2123345C1 (en) * 1997-07-31 1998-12-20 Научно-исследовательский институт детского питания Российской Академии сельскохозяйственных наук Agent for disbacteriosis treatment

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20160521

NF4A Reinstatement of patent

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20180521