RU2013123078A - METHOD FOR PRODUCING BIFIDOGENIC FACTOR - Google Patents
METHOD FOR PRODUCING BIFIDOGENIC FACTOR Download PDFInfo
- Publication number
- RU2013123078A RU2013123078A RU2013123078/10A RU2013123078A RU2013123078A RU 2013123078 A RU2013123078 A RU 2013123078A RU 2013123078/10 A RU2013123078/10 A RU 2013123078/10A RU 2013123078 A RU2013123078 A RU 2013123078A RU 2013123078 A RU2013123078 A RU 2013123078A
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- RU
- Russia
- Prior art keywords
- producing
- bifidogenic factor
- sepharose
- bifidogenic
- factor
- Prior art date
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Способ получения бифидогенного фактора, отличающийся тем, что в качестве бифидогенного фактора используется дезоксирибонуклеиновая кислота, которая выделяется из накопленной клеточной массы необходимого штамма бифидобактерий в процессе ультразвуковой обработки при частоте 40кГц с последующим отделением супернатанта центрифугированием при 5 тыс. об/мин и хроматографированием с использованием сефарозы (Sepharose) CL-4B.A method of producing a bifidogenic factor, characterized in that deoxyribonucleic acid is used as the bifidogenic factor, which is released from the accumulated cell mass of the required strain of bifidobacteria in the process of ultrasonic treatment at a frequency of 40 kHz, followed by separation of the supernatant by centrifugation at 5 thousand rpm and chromatography using sepharose (Sepharose) CL-4B.
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2013123078/10A RU2553513C2 (en) | 2013-05-20 | 2013-05-20 | Method of obtaining bifidogenic factor |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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RU2013123078/10A RU2553513C2 (en) | 2013-05-20 | 2013-05-20 | Method of obtaining bifidogenic factor |
Publications (2)
Publication Number | Publication Date |
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RU2013123078A true RU2013123078A (en) | 2014-11-27 |
RU2553513C2 RU2553513C2 (en) | 2015-06-20 |
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RU2013123078/10A RU2553513C2 (en) | 2013-05-20 | 2013-05-20 | Method of obtaining bifidogenic factor |
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RU (1) | RU2553513C2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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RU2738671C1 (en) * | 2020-02-26 | 2020-12-15 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кемеровский медицинский университет" (ФГБОУ ВО КемГМУ) | Method for producing and purifying biologically active substance produced by bifidobacteria |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
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SU1124033A1 (en) * | 1983-02-16 | 1984-11-15 | Предприятие П/Я Г-4740 | Method for preparing dna plasmid |
RU2123345C1 (en) * | 1997-07-31 | 1998-12-20 | Научно-исследовательский институт детского питания Российской Академии сельскохозяйственных наук | Agent for disbacteriosis treatment |
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- 2013-05-20 RU RU2013123078/10A patent/RU2553513C2/en not_active IP Right Cessation
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RU2553513C2 (en) | 2015-06-20 |
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Legal Events
Date | Code | Title | Description |
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MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20160521 |
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NF4A | Reinstatement of patent |
Effective date: 20170220 |
|
MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20180521 |