RU2013101312A - METHOD FOR PRODUCING REGENERANT PLANTS IN VITRO - Google Patents

METHOD FOR PRODUCING REGENERANT PLANTS IN VITRO Download PDF

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Publication number
RU2013101312A
RU2013101312A RU2013101312/10A RU2013101312A RU2013101312A RU 2013101312 A RU2013101312 A RU 2013101312A RU 2013101312/10 A RU2013101312/10 A RU 2013101312/10A RU 2013101312 A RU2013101312 A RU 2013101312A RU 2013101312 A RU2013101312 A RU 2013101312A
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RU
Russia
Prior art keywords
nutrient medium
vitro
formation
regenerants
explant
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RU2013101312/10A
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Russian (ru)
Inventor
Светлана Олеговна Володина
Владимир Витальевич Володин
Валерия Николаевна Орлова
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Федеральное государственное бюджетное учреждение науки Институт биологии Коми научного центра Уральского отделения РАН
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Application filed by Федеральное государственное бюджетное учреждение науки Институт биологии Коми научного центра Уральского отделения РАН filed Critical Федеральное государственное бюджетное учреждение науки Институт биологии Коми научного центра Уральского отделения РАН
Priority to RU2013101312/10A priority Critical patent/RU2013101312A/en
Publication of RU2013101312A publication Critical patent/RU2013101312A/en

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Abstract

Способ получения растений-регенерантов in vitro, включающий посадку экспланта на питательную среду, получение проростков на питательной среде для формирования регенерантов, отличающийся тем, что в качестве экспланта применяют незрелые семена, которые инкубируют в условиях темноты до образования протокормов, протокормы переносят на свет и культивируют до образования проростков, в качестве питательной среды для формирования регенерантов используют среду по Malmgren с добавлением аминоплазмаля в количестве 0,5-1,5 г/л.A method of producing regenerated plants in vitro, including planting an explant on a nutrient medium, obtaining seedlings on a nutrient medium for forming regenerants, characterized in that immature seeds are used as an explant, which are incubated in the dark until proto-feeds are formed, the proto-feeds are transferred to light and cultivated before the formation of seedlings, Malmgren medium with the addition of aminoplasmal in an amount of 0.5-1.5 g / l is used as a nutrient medium for the formation of regenerants.

Claims (1)

Способ получения растений-регенерантов in vitro, включающий посадку экспланта на питательную среду, получение проростков на питательной среде для формирования регенерантов, отличающийся тем, что в качестве экспланта применяют незрелые семена, которые инкубируют в условиях темноты до образования протокормов, протокормы переносят на свет и культивируют до образования проростков, в качестве питательной среды для формирования регенерантов используют среду по Malmgren с добавлением аминоплазмаля в количестве 0,5-1,5 г/л.A method of producing regenerated plants in vitro, including planting an explant on a nutrient medium, obtaining seedlings on a nutrient medium for the formation of regenerants, characterized in that immature seeds are used as an explant, which are incubated in the dark until protocorms are formed, the protocorms are transferred to light and cultivated before the formation of seedlings, Malmgren medium with the addition of aminoplasmal in an amount of 0.5-1.5 g / l is used as a nutrient medium for the formation of regenerants.
RU2013101312/10A 2013-01-10 2013-01-10 METHOD FOR PRODUCING REGENERANT PLANTS IN VITRO RU2013101312A (en)

Priority Applications (1)

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RU2013101312/10A RU2013101312A (en) 2013-01-10 2013-01-10 METHOD FOR PRODUCING REGENERANT PLANTS IN VITRO

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2013101312/10A RU2013101312A (en) 2013-01-10 2013-01-10 METHOD FOR PRODUCING REGENERANT PLANTS IN VITRO

Publications (1)

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RU2013101312A true RU2013101312A (en) 2014-07-20

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RU2013101312/10A RU2013101312A (en) 2013-01-10 2013-01-10 METHOD FOR PRODUCING REGENERANT PLANTS IN VITRO

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Effective date: 20140605