RU2012141175A - METHOD FOR DIAGNOSIS OF THE PATHOLOGY OF THE NERVO-MUSCULAR APPARATUS FOR NEURODEREGENERATIVE DISEASES IN GENETIC MODELS OF ALZHEIMER'S DISEASE AND LATE AMYOTROPHIC SCLEROSIS IN ANIMALS - Google Patents
METHOD FOR DIAGNOSIS OF THE PATHOLOGY OF THE NERVO-MUSCULAR APPARATUS FOR NEURODEREGENERATIVE DISEASES IN GENETIC MODELS OF ALZHEIMER'S DISEASE AND LATE AMYOTROPHIC SCLEROSIS IN ANIMALS Download PDFInfo
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Abstract
Способ диагностики патологии нервно-мышечного аппарата при нейродегенеративных заболеваниях в генетических моделях болезни Альцгеймера (БА) и бокового амиотрофического склероза (БАС) у трансгенных мышей, включающий исследование периферической ткани, отличающийся тем, что производят исследование тканей нервно-мышечной системы, а именно: во-первых, исследуют квантовый выброс нейромедиатора ацетилхолин в нервно-мышечном синапсе: у мышей с моделями БА и БАС определяется снижение выброса нейромедиатора в два раза; во-вторых, оценивают количество нервно-мышечных синапсов, в которых происходит загрузка флуоресцентного красителя FM 1-43, а также степень загрузки FM 1-43: у мышей с моделями БА и БАС происходит достоверное снижение количества синапсов с загрузкой FM 1-43 и/или снижается степень степень загрузки FM 1-43; в-третьих, исследуют метаболизм мембранного холестерина в нервно-мышечном синапсе: у мышей с моделями БА и БАС наблюдается нарушение распределения и уменьшение количества мембранного холестерина на 25-35% в сравнении с мышами дикого типа, также измеряют мембранный потенциал покоя клеток скелетных мышечных волокон: у мышей с моделями БА и БАС наблюдается снижение мембранного потенциала не менее чем на 10 мВ, в результате достигается выявление патологии нервно-мышечного аппарата при нейродегенеративных заболеваний в моделях БА и БАС у трансгенных мышей.A method for the diagnosis of pathology of the neuromuscular apparatus in neurodegenerative diseases in the genetic models of Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS) in transgenic mice, including the study of peripheral tissue, characterized in that the study of tissues of the neuromuscular system, namely: -first, they study the quantum emission of the neurotransmitter acetylcholine in the neuromuscular synapse: in mice with models of BA and ALS, a decrease in the emission of the neurotransmitter by half is determined; secondly, the number of neuromuscular synapses in which FM 1-43 fluorescent dye is loaded, as well as the degree of FM 1-43 loading, is estimated: in mice with BA and ALS models, there is a significant decrease in the number of synapses loaded with FM 1-43 and / or the degree of utilization of FM 1-43 is reduced; thirdly, they study the metabolism of membrane cholesterol in the neuromuscular synapse: mice with models of AD and ALS show a violation of the distribution and a decrease in the amount of membrane cholesterol by 25-35% in comparison with wild-type mice, and the membrane resting potential of skeletal muscle fiber cells is also measured : in mice with AD and ALS models, a decrease in the membrane potential of not less than 10 mV is observed; as a result, pathology of the neuromuscular system in neurodegenerative diseases is detected in AD and ALS models in trans ennyh mice.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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RU2755768C1 (en) * | 2021-03-09 | 2021-09-21 | Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр глазных болезней имени Гельмгольца" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ ГБ им. Гельмгольца" Минздрава России) | Method for predicting the development of fus-associated neurodegenerative disorders in mice |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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RU2755768C1 (en) * | 2021-03-09 | 2021-09-21 | Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр глазных болезней имени Гельмгольца" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ ГБ им. Гельмгольца" Минздрава России) | Method for predicting the development of fus-associated neurodegenerative disorders in mice |
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FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20150122 |