RU2012130988A - KIT FOR IDENTIFYING THE KU-Fever Causative Agent in Biological Material by the Polymerase Chain Reaction Method in Real Time (PCR-RV) - Google Patents
KIT FOR IDENTIFYING THE KU-Fever Causative Agent in Biological Material by the Polymerase Chain Reaction Method in Real Time (PCR-RV) Download PDFInfo
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- RU2012130988A RU2012130988A RU2012130988/10A RU2012130988A RU2012130988A RU 2012130988 A RU2012130988 A RU 2012130988A RU 2012130988/10 A RU2012130988/10 A RU 2012130988/10A RU 2012130988 A RU2012130988 A RU 2012130988A RU 2012130988 A RU2012130988 A RU 2012130988A
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Abstract
Набор для выявления возбудителя Ку-лихорадки в биологическом материале методом полимеразной цепной реакции в режиме реального времени (ПЦР-РВ), содержащий синтетические олигонуклеотиды и олигонуклеотид, меченый флуоресцентной меткой (флуоресцентный зонд), отличающийся тем, что синтетические олигонуклеотиды имеют следующую структуру:GroEL F 5' CTTCTACTGTTATGACGCCTTCTTTGC 3'GroEL R 5' CGCAAGTAGGCACCATTTCTGC 3',а флуоресцентный зонд -GroEL Probe 5' FAM-CACTTTCTCCATCGCTTCCGCAATAATA-TAMRA 3' Real-time polymerase chain reaction (PCR-RV) detection kit for Q fever pathogen in biological material containing synthetic oligonucleotides and an oligonucleotide labeled with a fluorescent label (fluorescent probe), characterized in that the synthetic oligonucleotides have the following structure: GroEL F 5 'CTTCTACTGTTATGACGCCTTCTTTGC 3'GroEL R 5' CGCAAGTAGGCACCATTTCTGC 3 'and fluorescent probe -GroEL Probe 5' FAM-CACTTTCTCCATCGCTTCCGCAATAATA-TAMRA 3 '
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RU2012130988/10A RU2525059C2 (en) | 2012-07-19 | 2012-07-19 | Kit for detecting q fever agent in biological material by real time polymerase chain reaction (rt pcr) |
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RU2012130988/10A RU2525059C2 (en) | 2012-07-19 | 2012-07-19 | Kit for detecting q fever agent in biological material by real time polymerase chain reaction (rt pcr) |
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RU2012130988A true RU2012130988A (en) | 2014-01-27 |
RU2525059C2 RU2525059C2 (en) | 2014-08-10 |
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RU2012130988/10A RU2525059C2 (en) | 2012-07-19 | 2012-07-19 | Kit for detecting q fever agent in biological material by real time polymerase chain reaction (rt pcr) |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112226527A (en) * | 2020-11-17 | 2021-01-15 | 武汉大学 | Method for detecting Coxix berber by TaqMan probe fluorescent quantitative PCR and application |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2004283153A (en) * | 2003-03-19 | 2004-10-14 | Shigeta Animal Pharmaceuticals Inc | Method for quickly detecting q fever bacillus in high accuracy by pcr (polymerase chain reaction) method |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112226527A (en) * | 2020-11-17 | 2021-01-15 | 武汉大学 | Method for detecting Coxix berber by TaqMan probe fluorescent quantitative PCR and application |
CN112226527B (en) * | 2020-11-17 | 2023-09-05 | 武汉大学 | Method for detecting Cookex body by TaqMan probe fluorescent quantitative PCR and application |
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RU2525059C2 (en) | 2014-08-10 |
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