RU2012130988A - KIT FOR IDENTIFYING THE KU-Fever Causative Agent in Biological Material by the Polymerase Chain Reaction Method in Real Time (PCR-RV) - Google Patents

KIT FOR IDENTIFYING THE KU-Fever Causative Agent in Biological Material by the Polymerase Chain Reaction Method in Real Time (PCR-RV) Download PDF

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RU2012130988A
RU2012130988A RU2012130988/10A RU2012130988A RU2012130988A RU 2012130988 A RU2012130988 A RU 2012130988A RU 2012130988/10 A RU2012130988/10 A RU 2012130988/10A RU 2012130988 A RU2012130988 A RU 2012130988A RU 2012130988 A RU2012130988 A RU 2012130988A
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RU
Russia
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pcr
chain reaction
polymerase chain
fever
biological material
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RU2012130988/10A
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Russian (ru)
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RU2525059C2 (en
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Ольга Александровна Фрейлихман
Николай Константинович Токаревич
Юлия Александровна Панфёрова
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Федеральное бюджетное учреждение науки "Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. Пастера" (ФБУН НИИ эпидемиологии и микробиологии имени Пастера)
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Priority to RU2012130988/10A priority Critical patent/RU2525059C2/en
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Abstract

Набор для выявления возбудителя Ку-лихорадки в биологическом материале методом полимеразной цепной реакции в режиме реального времени (ПЦР-РВ), содержащий синтетические олигонуклеотиды и олигонуклеотид, меченый флуоресцентной меткой (флуоресцентный зонд), отличающийся тем, что синтетические олигонуклеотиды имеют следующую структуру:GroEL F 5' CTTCTACTGTTATGACGCCTTCTTTGC 3'GroEL R 5' CGCAAGTAGGCACCATTTCTGC 3',а флуоресцентный зонд -GroEL Probe 5' FAM-CACTTTCTCCATCGCTTCCGCAATAATA-TAMRA 3' Real-time polymerase chain reaction (PCR-RV) detection kit for Q fever pathogen in biological material containing synthetic oligonucleotides and an oligonucleotide labeled with a fluorescent label (fluorescent probe), characterized in that the synthetic oligonucleotides have the following structure: GroEL F 5 'CTTCTACTGTTATGACGCCTTCTTTGC 3'GroEL R 5' CGCAAGTAGGCACCATTTCTGC 3 'and fluorescent probe -GroEL Probe 5' FAM-CACTTTCTCCATCGCTTCCGCAATAATA-TAMRA 3 '

Claims (1)

Набор для выявления возбудителя Ку-лихорадки в биологическом материале методом полимеразной цепной реакции в режиме реального времени (ПЦР-РВ), содержащий синтетические олигонуклеотиды и олигонуклеотид, меченый флуоресцентной меткой (флуоресцентный зонд), отличающийся тем, что синтетические олигонуклеотиды имеют следующую структуру:A kit for detecting the causative agent of Q fever in biological material by real-time polymerase chain reaction (PCR-RV), containing synthetic oligonucleotides and an oligonucleotide labeled with a fluorescent label (fluorescent probe), characterized in that the synthetic oligonucleotides have the following structure: GroEL F 5' CTTCTACTGTTATGACGCCTTCTTTGC 3'GroEL F 5 'CTTCTACTGTTATGACGCCTTCTTTGC 3' GroEL R 5' CGCAAGTAGGCACCATTTCTGC 3',GroEL R 5 'CGCAAGTAGGCACCATTTCTGC 3', а флуоресцентный зонд -and a fluorescent probe - GroEL Probe 5' FAM-CACTTTCTCCATCGCTTCCGCAATAATA-TAMRA 3' GroEL Probe 5 'FAM-CACTTTCTCCATCGCTTCCGCAATAATA-TAMRA 3'
RU2012130988/10A 2012-07-19 2012-07-19 Kit for detecting q fever agent in biological material by real time polymerase chain reaction (rt pcr) RU2525059C2 (en)

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RU2012130988/10A RU2525059C2 (en) 2012-07-19 2012-07-19 Kit for detecting q fever agent in biological material by real time polymerase chain reaction (rt pcr)

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RU2012130988/10A RU2525059C2 (en) 2012-07-19 2012-07-19 Kit for detecting q fever agent in biological material by real time polymerase chain reaction (rt pcr)

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RU2012130988A true RU2012130988A (en) 2014-01-27
RU2525059C2 RU2525059C2 (en) 2014-08-10

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112226527A (en) * 2020-11-17 2021-01-15 武汉大学 Method for detecting Coxix berber by TaqMan probe fluorescent quantitative PCR and application

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004283153A (en) * 2003-03-19 2004-10-14 Shigeta Animal Pharmaceuticals Inc Method for quickly detecting q fever bacillus in high accuracy by pcr (polymerase chain reaction) method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112226527A (en) * 2020-11-17 2021-01-15 武汉大学 Method for detecting Coxix berber by TaqMan probe fluorescent quantitative PCR and application
CN112226527B (en) * 2020-11-17 2023-09-05 武汉大学 Method for detecting Cookex body by TaqMan probe fluorescent quantitative PCR and application

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RU2525059C2 (en) 2014-08-10

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PD4A Correction of name of patent owner