RU2011118970A - DIAGNOSTIC TEST FOR IDENTIFICATION OF MITOCHONDRIAL ANIMAL DNA IN FODDER AND MEAT PRODUCTS - Google Patents
DIAGNOSTIC TEST FOR IDENTIFICATION OF MITOCHONDRIAL ANIMAL DNA IN FODDER AND MEAT PRODUCTS Download PDFInfo
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- RU2011118970A RU2011118970A RU2011118970/10A RU2011118970A RU2011118970A RU 2011118970 A RU2011118970 A RU 2011118970A RU 2011118970/10 A RU2011118970/10 A RU 2011118970/10A RU 2011118970 A RU2011118970 A RU 2011118970A RU 2011118970 A RU2011118970 A RU 2011118970A
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Abstract
1. Диагностический тест для выявления митохондриальной ДНК животных в кормах и мясных продуктах, содержащий первый комплект для выделения ДНК, второй комплект, включающий праймеры для проведения мультипраймерной реакции амплификации методом ПЦР, третий комплект для проведения электрофореза в ПААГ, отличающийся тем, что второй комплект содержит первую и вторую композицию праймеров приведенных на фиг.1 и фиг.2 соответственно, при этом в первый набор входят праймеры с нуклеотидной последовательностью SEQ ID NO: 1 по SEQ ID NO: 10 комплементарные к участкам ДНК, входящим в группу состоящей из: Sus scrofa domesticus(свинья домашняя), Bos taurus taurus(домашний бык), Gallus gallus (домашняя курица), Ovis aries(домашняя овца), Meleagris gallopavo(обыкновенная индейка) и праймеры внутреннего контроля SEQ ID NO: 20 и SEQ ID NO: 21, а во второй набор входят олигонуклеотиды SEQ ID NO: 11 по SEQ ID NO: 19 комплементарные к участкам ДНК входящим в группу, состоящей из: Mus musculus domesticus(мышь домовая), Rattus rattus (norvegicus)(крыса черная (серая)), Canis lupus familiaris(собака домашняя), Homo sapiens(человек разумный), Felis catus(домашняя кошка) и праймеры внутреннего контроля SEQ ID NO: 20 и SEQ ID NO: 21, при этом второй комплект дополнительно содержит положительный и отрицательный контрольные образцы.2. Диагностический тест по п.1, отличающийся тем, что положительный контрольный образец содержит смесь из десяти рекомбинантных плазмид, выполненных на основе плазмиды pUC18, при этом каждая плазмида содержит последовательность одного из маркерных генов, входящих в группу Sus scrofa domesticus, Bos taurus taunts, Gallus gallus, Ovis aries, Meleagris gallopavo Mus musculus domesticus, Rattus rattus, Canis lupus familiaris, Homo sapiens, Felis catus.3. Диагностический тест по п.1, отличающийся тем, что отрицательный элемент контр�1. Diagnostic test for detecting mitochondrial DNA of animals in feed and meat products, containing the first kit for DNA isolation, the second kit, including primers for conducting a multi-primer amplification reaction by PCR, the third kit for electrophoresis in SDS page, characterized in that the second kit contains the first and second composition of the primers shown in figure 1 and figure 2, respectively, while the first set includes primers with the nucleotide sequence of SEQ ID NO: 1 according to SEQ ID NO: 10 complementary to DNA strands included in the group consisting of: Sus scrofa domesticus (domestic pig), Bos taurus taurus (domestic bull), Gallus gallus (domestic chicken), Ovis aries (domestic sheep), Meleagris gallopavo (common turkey) and SEQ internal control primers ID NO: 20 and SEQ ID NO: 21, and the second set includes the oligonucleotides SEQ ID NO: 11 according to SEQ ID NO: 19 complementary to the DNA regions included in the group consisting of: Mus musculus domesticus (house mouse), Rattus rattus ( norvegicus) (rat black (gray)), Canis lupus familiaris (domestic dog), Homo sapiens (intelligent human), Felis catus (domestic cat) and internal control primers SEQ ID NO: 20 and SEQ ID NO: 21, with that the second set further includes positive and negative control obraztsy.2. The diagnostic test according to claim 1, characterized in that the positive control sample contains a mixture of ten recombinant plasmids made on the basis of plasmid pUC18, each plasmid containing a sequence of one of the marker genes belonging to the group Sus scrofa domesticus, Bos taurus taunts, Gallus gallus, Ovis aries, Meleagris gallopavo Mus musculus domesticus, Rattus rattus, Canis lupus familiaris, Homo sapiens, Felis catus. 3. The diagnostic test according to claim 1, characterized in that the negative element of the control�
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RU2011118970/10A RU2560579C2 (en) | 2011-05-12 | 2011-05-12 | Set of oligonucleotide primers for identification of animal dna in forage and meat products |
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RU2011118970/10A RU2560579C2 (en) | 2011-05-12 | 2011-05-12 | Set of oligonucleotide primers for identification of animal dna in forage and meat products |
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RU2678090C1 (en) * | 2018-05-28 | 2019-01-23 | Федеральное государственное бюджетное научное учреждение "Федеральный научный центр пищевых систем им. В.М. Горбатова" РАН | Boiled sausage products authenticity confirmation method |
RU2725215C1 (en) * | 2019-10-14 | 2020-06-30 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" | Test system for identification of common hedgehog (erinaceus europaeus) tissue dna in dry fodder and semi-finished meat products |
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RU2310686C2 (en) * | 1999-06-25 | 2007-11-20 | Басф Акциенгезелльшафт | Corynebacterium glutamicum genes encoding proteins participating in carbon metabolism and energy producing |
RU2259398C1 (en) * | 2003-12-18 | 2005-08-27 | Государственное Научное Учреждение Институт экспериментальной ветеринарии Сибири и Дальнего Востока СО РАСХН (ГНУ ИЭВСиДВ СО РАСХН) | Synthetic oligonucleotide primers and method for detecting cattle infectious rhinotracheitis virus dna using specific oligonucleotide primers in polymerase chain reaction (pcr) |
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