RU2011107212A - MICROBIOLOGICAL METHOD FOR PRODUCING 1,2-PROPANDIOL - Google Patents

MICROBIOLOGICAL METHOD FOR PRODUCING 1,2-PROPANDIOL Download PDF

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RU2011107212A
RU2011107212A RU2011107212/10A RU2011107212A RU2011107212A RU 2011107212 A RU2011107212 A RU 2011107212A RU 2011107212/10 A RU2011107212/10 A RU 2011107212/10A RU 2011107212 A RU2011107212 A RU 2011107212A RU 2011107212 A RU2011107212 A RU 2011107212A
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host cell
activity
cell according
propanediol
genetic engineering
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RU2011107212/10A
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RU2521502C2 (en
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Йорг МАМПЕЛЬ (DE)
Йорг МАМПЕЛЬ
Гвидо МОЙРЕР (DE)
Гвидо МОЙРЕР
Юрген ЭК (DE)
Юрген ЭК
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Клариант Финанс (Бви) Лимитед (Vg)
Клариант Финанс (Бви) Лимитед
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • General Chemical & Material Sciences (AREA)
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  • Biochemistry (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1. Клетка-хозяин, созданная методами генной инженерии для выработки высоких уровней 1,2-пропандиола при выращивании на глицерине как единственном источнике углерода. ! 2. Клетка-хозяин по п.1, где глицерин имеет степень чистоты от 80% до 90%. ! 3. Клетка-хозяин по п.1, созданная методами генной инженерии путем введения гена, кодирующего активность пропандиолоксидоредуктазы. ! 4. Клетка-хозяин по п.3, созданная методами генной инженерии путем введения, по меньшей мере, одного дополнительного гена, кодирующего ферментативную активность, выбранную из группы, состоящей из глицеролдегидрогеназы (gldA), дигидроксиапетонкиназы (dhaK) и метилглиоксальсинтазы (mgsA), с тем, чтобы экспрессировать указанные активности вместе с активностью пропандиолоксидоредуктазы (fucO). ! 5. Клетка-хозяин по п.3, созданная методами генной инженерии путем введения дополнительных генов, кодирующих глицеролдегидрогеназу, дигидроксиацетонкиназу и метилглиоксальсинтазу, с тем, чтобы экспрессировать указанные активности глицеролдегидрогеназы, дигидроксиацетонкиназы и метилглиоксальсинтазы вместе с активностью пропандиолоксидоредуктазы. ! 6. Клетка-хозяин по п.3, созданная методами генной инженерии путем введения дополнительных генов, кодирующих глицеролдегидратазу, с тем, чтобы экспрессировать указанную активность глицеролдегидратазы вместе с активностью пропандиолоксидоредуктазы. ! 7. Клетка-хозяин по п.3, созданная методами генной инженерии путем введения дополнительных генов, кодирующих альдокеторедуктазу, с тем, чтобы экспрессировать указанную активность альдокеторедуктазы вместе с активностью пропандиолоксидоредуктазы. ! 8. Клетка-хозяин п� 1. A host cell genetically engineered to produce high levels of 1,2-propanediol when grown on glycerol as the sole carbon source. ! 2. The host cell of claim 1, wherein the glycerol is 80% to 90% pure. ! 3. A host cell according to claim 1, created by genetic engineering methods by introducing a gene encoding propanediol oxidoreductase activity. ! 4. A host cell according to claim 3, generated by genetic engineering methods by introducing at least one additional gene encoding an enzymatic activity selected from the group consisting of glycerol dehydrogenase (gldA), dihydroxyapetone kinase (dhaK) and methylglyoxal synthase (mgsA), in order to express these activities together with the activity of propanediol oxidoreductase (fucO). ! 5. A host cell according to claim 3, generated by genetic engineering methods by introducing additional genes encoding glycerol dehydrogenase, dihydroxyacetone kinase, and methylglyoxal synthase, so as to express said activities of glycerol dehydrogenase, dihydroxyacetone oxidase and methylglyoxal synthase activity together with propylene glyoxal synthase activity. ! 6. A host cell according to claim 3, genetically engineered by introducing additional genes encoding glycerol dehydratase in order to express said glycerol dehydratase activity together with propanediol oxidoreductase activity. ! 7. A host cell according to claim 3, generated by genetic engineering methods by introducing additional genes encoding aldoketo reductase in order to express said aldoketo reductase activity together with propanediol oxidoreductase activity. ! 8. The host cell is

Claims (11)

1. Клетка-хозяин, созданная методами генной инженерии для выработки высоких уровней 1,2-пропандиола при выращивании на глицерине как единственном источнике углерода.1. A host cell created by genetic engineering to generate high levels of 1,2-propanediol when grown on glycerol as the sole carbon source. 2. Клетка-хозяин по п.1, где глицерин имеет степень чистоты от 80% до 90%.2. The host cell according to claim 1, where the glycerin has a degree of purity from 80% to 90%. 3. Клетка-хозяин по п.1, созданная методами генной инженерии путем введения гена, кодирующего активность пропандиолоксидоредуктазы.3. The host cell according to claim 1, created by genetic engineering by introducing a gene encoding the activity of propanediol oxidoreductase. 4. Клетка-хозяин по п.3, созданная методами генной инженерии путем введения, по меньшей мере, одного дополнительного гена, кодирующего ферментативную активность, выбранную из группы, состоящей из глицеролдегидрогеназы (gldA), дигидроксиапетонкиназы (dhaK) и метилглиоксальсинтазы (mgsA), с тем, чтобы экспрессировать указанные активности вместе с активностью пропандиолоксидоредуктазы (fucO).4. The host cell according to claim 3, created by genetic engineering by introducing at least one additional gene encoding the enzymatic activity selected from the group consisting of glycerol dehydrogenase (gldA), dihydroxyapetonokinase (dhaK) and methylglyoxal synthase (mgsA), in order to express these activities together with the activity of propanediol oxidoreductase (fucO). 5. Клетка-хозяин по п.3, созданная методами генной инженерии путем введения дополнительных генов, кодирующих глицеролдегидрогеназу, дигидроксиацетонкиназу и метилглиоксальсинтазу, с тем, чтобы экспрессировать указанные активности глицеролдегидрогеназы, дигидроксиацетонкиназы и метилглиоксальсинтазы вместе с активностью пропандиолоксидоредуктазы.5. The host cell according to claim 3, created by genetic engineering by introducing additional genes encoding glycerol dehydrogenase, dihydroxyacetonkinase and methylglyoxal synthase, in order to express the indicated activities of glycerol dehydrogenase, dihydroxyacetone kinase and methylglyoxides synthase synthase. 6. Клетка-хозяин по п.3, созданная методами генной инженерии путем введения дополнительных генов, кодирующих глицеролдегидратазу, с тем, чтобы экспрессировать указанную активность глицеролдегидратазы вместе с активностью пропандиолоксидоредуктазы.6. The host cell according to claim 3, created by genetic engineering by introducing additional genes encoding glycerol dehydrate in order to express the indicated glycerol dehydratase activity together with the activity of propanediol oxidoreductase. 7. Клетка-хозяин по п.3, созданная методами генной инженерии путем введения дополнительных генов, кодирующих альдокеторедуктазу, с тем, чтобы экспрессировать указанную активность альдокеторедуктазы вместе с активностью пропандиолоксидоредуктазы.7. The host cell according to claim 3, created by genetic engineering by introducing additional genes encoding aldoketoreductase in order to express the indicated activity of aldoketoreductase along with the activity of propanediol oxidoreductase. 8. Клетка-хозяин по п.1, дефектная, по меньшей мере, по метаболизму соединений, выбранных из группы, состоящей из8. The host cell according to claim 1, defective, at least in the metabolism of compounds selected from the group consisting of i) арабинозы,i) arabinose, ii) метилглиоксаля,ii) methylglyoxal, iii) дигидроксиацетонфосфата.iii) dihydroxyacetone phosphate. 9. Клетка-хозяин по любому из предшествующих пп., вырабатывающая высокие уровни 1,2-пропандиола при выращивании на глицерине как единственном источнике углерода, но по существу, без 1,3-пропандиола.9. A host cell according to any one of the preceding claims, producing high levels of 1,2-propanediol when grown on glycerin as the sole carbon source, but essentially without 1,3-propanediol. 10. Клетка-хозяин по п.9, представляющая собой Е.coli.10. The host cell according to claim 9, which is an E. coli. 11. Способ получения 1,2-пропандиола, включающий выращивание клеток-хозяев по любому из пп.1-10 в соответствующей среде для выращивания, содержащей глицерин, после чего выработанный 1,2-пропандиол извлекают и, необязательно, очищают. 11. A method for producing 1,2-propanediol, comprising growing host cells according to any one of claims 1 to 10 in an appropriate growth medium containing glycerin, after which the generated 1,2-propanediol is recovered and, optionally, purified.
RU2011107212/10A 2008-07-28 2009-07-16 Microbiological method of obtaining 1,2-propanediole RU2521502C2 (en)

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EP08161267.3 2008-07-28
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CA2732204A1 (en) 2010-02-04
DK2313489T3 (en) 2015-06-22
RU2521502C2 (en) 2014-06-27
ZA201007720B (en) 2011-07-27
WO2010012604A1 (en) 2010-02-04
CN102089421B (en) 2013-06-19
AU2009276074A1 (en) 2010-02-04
EP2313489B1 (en) 2015-04-29
JP2011528915A (en) 2011-12-01
JP5750370B2 (en) 2015-07-22

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