RU2010149317A - METHOD FOR QUANTITATIVE DETERMINATION OF SPECIFIC IMMUNOGLOBULINS G TO LOW-MOLECULAR COMPOUNDS IN BLOOD SERUM - Google Patents

Abstract

 1. A method for the quantitative determination of specific immunoglobulins G to low molecular weight compounds in blood serum, comprising introducing into the test blood serum a material containing the test low molecular weight compound, and determining the content of immunoglobulins G in the sample by enzyme immunoassay, characterized in that experimental and control nitrocellulose substrate, which is placed in different wells of the microplate and produce their preparation, and the preparation is experienced the second substrate is carried out by sequential sorption on it of human serum albumin and the test low molecular weight compound, and the preparation of the control substrate is carried out by sorption on it human serum albumin, then the test and control substrates are incubated with the test serum sample, then after washing the substrates with phosphate buffer, they are added to them well conjugated with horseradish peroxidase of monoclonal antibodies to the Fc fragment of human immunoglobulin G, again produce washing the substrates with phosphate buffer and the resulting immunochemical complex of specific immunoglobulin G antibodies is stained with chromogen and manifested with stop reagent, then the used substrates are removed from the microplate wells and the optical density in the experimental and control samples is recorded, and they are compared with the optical density of standard samples with a known concentration of immunoglobin G, and the difference in the content of immunoglobulin G in the experimental and control samples judges the number of specific

Claims (2)

1. A method for the quantitative determination of specific immunoglobulins G to low molecular weight compounds in blood serum, comprising introducing into the test blood serum a material containing the test low molecular weight compound, and determining the content of immunoglobulins G in the sample by enzyme immunoassay, characterized in that experimental and control nitrocellulose substrate, which is placed in different wells of the microplate and produce their preparation, and the preparation is experienced the second substrate is carried out by sequential sorption on it of human serum albumin and the test low molecular weight compound, and the preparation of the control substrate is carried out by sorption on it human serum albumin, then the test and control substrates are incubated with the test serum sample, then after washing the substrates with phosphate buffer, they are added to them well conjugated with horseradish peroxidase of monoclonal antibodies to the Fc fragment of human immunoglobulin G, again produce washing the substrates with phosphate buffer and the resulting immunochemical complex of specific immunoglobulin G antibodies is stained with chromogen and manifested with stop reagent, then the used substrates are removed from the microplate wells and the optical density in the experimental and control samples is recorded, and they are compared with the optical density of standard samples with a known concentration of immunoglobin G, and the difference in the content of immunoglobulin G in the experimental and control samples judges the number of specific a low molecular weight compound in serum immunoglobulin G patient when performing simultaneous conditions of excess absorbance in the test sample over the control sample the optical density of not less than 1.5 times. 2. The method according to claim 1, characterized in that when using a metal salt as a low molecular weight compound, the preparation of the control substrate is carried out by sorption on it of human serum albumin and a salt solution that does not have antigenic properties and has an anion similar to the metal salt anion.