RU2010127878A - METHOD FOR IMMOBILIZING L-PHENYLALANINE-AMMONIUM-LYASE ON MAGNETIC NANOPARTICLES - Google Patents

METHOD FOR IMMOBILIZING L-PHENYLALANINE-AMMONIUM-LYASE ON MAGNETIC NANOPARTICLES Download PDF

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RU2010127878A
RU2010127878A RU2010127878/10A RU2010127878A RU2010127878A RU 2010127878 A RU2010127878 A RU 2010127878A RU 2010127878/10 A RU2010127878/10 A RU 2010127878/10A RU 2010127878 A RU2010127878 A RU 2010127878A RU 2010127878 A RU2010127878 A RU 2010127878A
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Russia
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immobilizing
phenylalanine
magnetic nanoparticles
lyase
ammonium
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RU2010127878/10A
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Russian (ru)
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RU2460790C2 (en
Inventor
Ольга Олеговна Бабич (RU)
Ольга Олеговна Бабич
Любовь Сергеевна Солдатова (RU)
Любовь Сергеевна Солдатова
Александр Юрьевич Просеков (RU)
Александр Юрьевич Просеков
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Государственное образовательное учреждение высшего профессионального образования "Кемеровский технологический институт пищевой про
Государственное образовательное учреждение высшего профессионального образования "Кемеровский технологический институт пищевой промышленности"
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Priority to RU2010127878/10A priority Critical patent/RU2460790C2/en
Publication of RU2010127878A publication Critical patent/RU2010127878A/en
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  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Способ иммобилизации L-фенилаланин-аммоний-лиазы на магнитных наночастицах, заключающийся в ковалентном связывании молекул фермента с поверхностью инертного носителя посредством конденсирующего агента, отличающийся тем, что в качестве поверхностно-модифицированного носителя выступают наночастицы оксидов металлов, а в качестве конденсирующего агента используют 1-этил-3-(3-диметиламинопропил) карбодиимид. A method of immobilizing L-phenylalanine-ammonium lyase on magnetic nanoparticles, which involves covalently bonding enzyme molecules to the surface of an inert carrier by means of a condensing agent, characterized in that metal oxide nanoparticles act as a surface-modified carrier, and 1- is used as a condensing agent ethyl 3- (3-dimethylaminopropyl) carbodiimide.

Claims (1)

Способ иммобилизации L-фенилаланин-аммоний-лиазы на магнитных наночастицах, заключающийся в ковалентном связывании молекул фермента с поверхностью инертного носителя посредством конденсирующего агента, отличающийся тем, что в качестве поверхностно-модифицированного носителя выступают наночастицы оксидов металлов, а в качестве конденсирующего агента используют 1-этил-3-(3-диметиламинопропил) карбодиимид. A method of immobilizing L-phenylalanine-ammonium lyase on magnetic nanoparticles, which involves covalently bonding enzyme molecules to the surface of an inert carrier by means of a condensing agent, characterized in that metal oxide nanoparticles act as a surface-modified carrier, and 1- is used as a condensing agent ethyl 3- (3-dimethylaminopropyl) carbodiimide.
RU2010127878/10A 2010-07-06 2010-07-06 Method for immobilisation of l-phenylalanine-ammonium-lyase on magnetic nanoparticles RU2460790C2 (en)

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RU2010127878/10A RU2460790C2 (en) 2010-07-06 2010-07-06 Method for immobilisation of l-phenylalanine-ammonium-lyase on magnetic nanoparticles

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RU2010127878/10A RU2460790C2 (en) 2010-07-06 2010-07-06 Method for immobilisation of l-phenylalanine-ammonium-lyase on magnetic nanoparticles

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RU2551317C2 (en) * 2013-04-10 2015-05-20 Федеральное государственное бюджетное учреждение науки Институт высокомолекулярных соединений Российской академии наук Method of immobilisation of chymotrypsin on nanoparticles of selenium or silver

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EP0230649A1 (en) * 1986-01-02 1987-08-05 Miles Inc. Immobilization of phenylaline ammonia-lyase

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