RU2009133970A - METHOD FOR PRODUCING GENETICALLY MODIFIED CELLS MEDICINAL PRODUCT AND ITS APPLICATION - Google Patents
METHOD FOR PRODUCING GENETICALLY MODIFIED CELLS MEDICINAL PRODUCT AND ITS APPLICATION Download PDFInfo
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- RU2009133970A RU2009133970A RU2009133970/10A RU2009133970A RU2009133970A RU 2009133970 A RU2009133970 A RU 2009133970A RU 2009133970/10 A RU2009133970/10 A RU 2009133970/10A RU 2009133970 A RU2009133970 A RU 2009133970A RU 2009133970 A RU2009133970 A RU 2009133970A
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- genetically modified
- drug
- modified cells
- cells
- fgf2
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1. Способ получения лекарственного препарата генетически модифицированных клеток и его применения, заключающийся в том, что получают двухкассетную генетическую плазмидную конструкцию pBud-VEGF-FGF2, содержащую фрагменты ДНК, кодирующие VEGF и FGF2, из биологической ткани человека выделяют мононуклеарные клетки, выделенные клетки генетически модифицируют генетической конструкцией pBud-VEGF-FGF2, генетически модифицированные клетки культивируют, концентрируют, очищают от культуральной среды, ресуспензируют, осуществляют анализ генетически модифицированных клеток на количество, концентрацию и жизнеспособность, по результатам анализа определяют оптимальную для конкретного применения терапевтическую дозу, которая является лекарственным препаратом генетически модифицированных клеток, препарат применяют по назначению. ! 2. Лекарственный препарат по п.1 применяют для лечения ишемических заболеваний людей и животных. ! 3. Лекарственный препарат по п.1 замораживают для хранения и последующего применения. 1. A method of obtaining a drug of genetically modified cells and its use, which consists in obtaining a two-cassette genetic plasmid construct pBud-VEGF-FGF2 containing DNA fragments encoding VEGF and FGF2, mononuclear cells are isolated from human biological tissue, isolated cells are genetically modified pBud-VEGF-FGF2 genetic construct, genetically modified cells are cultured, concentrated, purified from the culture medium, resuspended, genetically modified cells for the number, concentration and viability, according to the results of the analysis, the therapeutic dose optimal for a particular application is determined, which is a drug of genetically modified cells, the drug is used as directed. ! 2. The drug according to claim 1 is used to treat ischemic diseases of humans and animals. ! 3. The drug according to claim 1 is frozen for storage and subsequent use.
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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RU2009133970/10A RU2009133970A (en) | 2009-09-11 | 2009-09-11 | METHOD FOR PRODUCING GENETICALLY MODIFIED CELLS MEDICINAL PRODUCT AND ITS APPLICATION |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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RU2009133970/10A RU2009133970A (en) | 2009-09-11 | 2009-09-11 | METHOD FOR PRODUCING GENETICALLY MODIFIED CELLS MEDICINAL PRODUCT AND ITS APPLICATION |
Publications (1)
Publication Number | Publication Date |
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RU2009133970A true RU2009133970A (en) | 2011-03-20 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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RU2009133970/10A RU2009133970A (en) | 2009-09-11 | 2009-09-11 | METHOD FOR PRODUCING GENETICALLY MODIFIED CELLS MEDICINAL PRODUCT AND ITS APPLICATION |
Country Status (1)
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RU (1) | RU2009133970A (en) |
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2009
- 2009-09-11 RU RU2009133970/10A patent/RU2009133970A/en not_active Application Discontinuation
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20130404 |