RU2008137068A - METHOD FOR DETERMINING DNA-HYDROLYSIS ACTIVITY OF MOLECULES AND A DEVICE FOR ITS IMPLEMENTATION - Google Patents
METHOD FOR DETERMINING DNA-HYDROLYSIS ACTIVITY OF MOLECULES AND A DEVICE FOR ITS IMPLEMENTATION Download PDFInfo
- Publication number
- RU2008137068A RU2008137068A RU2008137068/13A RU2008137068A RU2008137068A RU 2008137068 A RU2008137068 A RU 2008137068A RU 2008137068/13 A RU2008137068/13 A RU 2008137068/13A RU 2008137068 A RU2008137068 A RU 2008137068A RU 2008137068 A RU2008137068 A RU 2008137068A
- Authority
- RU
- Russia
- Prior art keywords
- dna
- molecules
- modified
- electrode
- activity
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1. Способ определения ДНК-гидролизующей активности молекул, отличающийся тем, что определение осуществляют электрохимически, адсорбируя продукты гидролиза ДНК на рабочем электроде и регистрируя сигнал окисления продуктов гидролиза ДНК, величина которого пропорциональна ДНК-гидролизующей активности молекул. ! 2. Способ по п.1, отличающийся тем, что ДНК-гидролизующую активность молекул определяют в составе биологических материалов. ! 3. Способ по п.1, отличающийся тем, что ДНК-гидролизующую активность определяют у молекул, выделенных из биологических материалов. ! 4. Способ по п.1, отличающийся тем, что ДНК-гидролизующую активность определяют у небиологических молекул. ! 5. Устройство для проведения электрохимического анализа - электрохимический анализатор, отличающийся тем, что его применяют для определения активности ДНК-гидролизующих молекул. ! 6. Устройство по п.5, отличающееся тем, что используют модифицированный рабочий электрод. ! 7. Устройство по п.5, отличающееся тем, что используют электрод, модифицированный наноматериалами. ! 8. Устройство по пп.5 и 7, отличающееся тем, что используют электрод, модифицированный нанотрубками. ! 9. Устройство по пп.5 и 7, отличающееся тем, что используют электрод, модифицированный нановолокнами. ! 10. Устройство по пп.5 и 7, отличающееся тем, что используют электрод, модифицированный наноконусами. ! 11. Устройство по пп.5 и 7, отличающееся тем, что используют электрод, модифицированный углеродными наноматериалами.1. The method of determining the DNA hydrolyzing activity of molecules, characterized in that the determination is carried out electrochemically, adsorbing the products of DNA hydrolysis on the working electrode and registering the oxidation signal of the products of DNA hydrolysis, the magnitude of which is proportional to the DNA hydrolyzing activity of the molecules. ! 2. The method according to claim 1, characterized in that the DNA-hydrolyzing activity of the molecules is determined in the composition of biological materials. ! 3. The method according to claim 1, characterized in that the DNA hydrolyzing activity is determined in molecules isolated from biological materials. ! 4. The method according to claim 1, characterized in that the DNA hydrolyzing activity is determined in non-biological molecules. ! 5. Device for conducting electrochemical analysis - an electrochemical analyzer, characterized in that it is used to determine the activity of DNA hydrolyzing molecules. ! 6. The device according to claim 5, characterized in that a modified working electrode is used. ! 7. The device according to claim 5, characterized in that they use an electrode modified with nanomaterials. ! 8. The device according to PP.5 and 7, characterized in that they use an electrode modified with nanotubes. ! 9. The device according to PP.5 and 7, characterized in that they use an electrode modified with nanofibres. ! 10. The device according to PP.5 and 7, characterized in that they use an electrode modified with nanocones. ! 11. The device according to PP.5 and 7, characterized in that they use an electrode modified with carbon nanomaterials.
Claims (11)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2008137068/10A RU2413768C2 (en) | 2008-09-15 | 2008-09-15 | Method of determining dna-hydrolysing activity of molecules and device for its realisation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2008137068/10A RU2413768C2 (en) | 2008-09-15 | 2008-09-15 | Method of determining dna-hydrolysing activity of molecules and device for its realisation |
Publications (2)
Publication Number | Publication Date |
---|---|
RU2008137068A true RU2008137068A (en) | 2010-03-20 |
RU2413768C2 RU2413768C2 (en) | 2011-03-10 |
Family
ID=42137025
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2008137068/10A RU2413768C2 (en) | 2008-09-15 | 2008-09-15 | Method of determining dna-hydrolysing activity of molecules and device for its realisation |
Country Status (1)
Country | Link |
---|---|
RU (1) | RU2413768C2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2507512C2 (en) * | 2012-04-06 | 2014-02-20 | Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Уральский федеральный университет имени первого Президента России Б.Н. Ельцина" | Method of making modified electrode for electrochemical analysis (versions) |
-
2008
- 2008-09-15 RU RU2008137068/10A patent/RU2413768C2/en active
Also Published As
Publication number | Publication date |
---|---|
RU2413768C2 (en) | 2011-03-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Lahcen et al. | Label-free electrochemical sensor based on spore-imprinted polymer for Bacillus cereus spore detection | |
EP2419733A4 (en) | Methods and devices for detecting the presence of an analyte in a sample | |
CN103675066B (en) | A kind of preparation of sulfhydrylation graphene modified electrode and trace heavy metal detection method thereof | |
CN102253092B (en) | Composite film modified DNA sensor and its preparation method and application in detection of lignin peroxidase (Lip) specific coding gene segment | |
ATE532063T1 (en) | ELECTROCHEMICAL BIOSENSOR | |
CA2870354C (en) | Electrical impedance hematocrit and hba1c biosensor comprising sample plate and sample apparatus | |
EP3147372A3 (en) | Versatile and sensitive biosensor | |
Li et al. | Porous graphene oxide enhanced aptamer specific circulating-tumor-cell sensing interface on light addressable potentiometric sensor: clinical application and simulation | |
CY1115380T1 (en) | METHOD AND DEVELOPMENT FOR THE AID OF ELECTRO-PRODUCED NANOSOMATIC CHEMISTRY | |
WO2008157403A3 (en) | Method and apparatus for metal nanoparticle electrocatalytic amplification | |
IN2014CN03508A (en) | ||
RU2013118341A (en) | GETTING SERUM | |
CN102680549B (en) | A kind of 9-hydroxyphenyl fluorene assay method based on galvanochemistry hairpin DNA biosensor | |
Lian et al. | DNA wrapped metallic single-walled carbon nanotube sensor for Pb (II) detection | |
CN103901087B (en) | Modified electrode for detecting pyrocatechol and hydroquinone and preparation method thereof | |
SG159463A1 (en) | Method for detecting biomolecules and use thereof | |
CN105424787A (en) | Preparation method of silk screen printing sensor for detecting tetracycline and oxytetracycline at same time | |
Zhao et al. | An electrochemical sensor for l-tryptophan using a molecularly imprinted polymer film produced by copolymerization of o-phenylenediamine and hydroquinone | |
Zhou et al. | Stereoselectivity of tyrosine enantiomers in electrochemical redox reactions on gold matrices | |
JP2011058900A (en) | Redox species concentration measurement method | |
EP1746413A3 (en) | Electrochemical Biosensor and method of manufacture | |
EP2381253A3 (en) | Apparatus for detecting toxicity in water using sulfur-oxidizing bacteria | |
RU2008137068A (en) | METHOD FOR DETERMINING DNA-HYDROLYSIS ACTIVITY OF MOLECULES AND A DEVICE FOR ITS IMPLEMENTATION | |
CN203275352U (en) | Heavy metal ion detector | |
CN103884748A (en) | Preparation method of molecular imprinting electrochemical sensor for detecting serotonins |