RU2007146813A - METHOD FOR DETERMINING THE CONCENTRATION OF BENZYLPENICILLINE DRUGS IN BIOLOGICAL LIQUIDS - Google Patents

METHOD FOR DETERMINING THE CONCENTRATION OF BENZYLPENICILLINE DRUGS IN BIOLOGICAL LIQUIDS Download PDF

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RU2007146813A
RU2007146813A RU2007146813/15A RU2007146813A RU2007146813A RU 2007146813 A RU2007146813 A RU 2007146813A RU 2007146813/15 A RU2007146813/15 A RU 2007146813/15A RU 2007146813 A RU2007146813 A RU 2007146813A RU 2007146813 A RU2007146813 A RU 2007146813A
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agar
test
concentration
determining
standard solutions
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RU2007146813/15A
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Russian (ru)
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RU2379686C2 (en
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Надежда Константиновна Левчик (RU)
Надежда Константиновна Левчик
Евгения Валерьевна Мельникова (RU)
Евгения Валерьевна Мельникова
Марина Владиславовна Пономарева (RU)
Марина Владиславовна Пономарева
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Федеральное агентство по высокотехнологичной медицинской помощи Федеральное государственное учреждение "Уральский научно-исследоват
Федеральное агентство по высокотехнологичной медицинской помощи Федеральное государственное учреждение "Уральский научно-исследовательский институт дерматовенерологии и иммунопатологии" (ФГУ УрНИИДВиИ Росмедтехнологий)
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Abstract

Способ определения концентрации препаратов бензилпенициллина в биологических жидкостях путем использования микробиологического метода диффузии в агар, включающий приготовление препарата тест-микроба, создание слоя агара, вырезание в застывшем агаре лунок, введение в них растворов стандарта и предварительное разведение испытуемых образцов, отличающийся тем, что в качестве тест-микроба используют Staphylococcus aureus ATCC 25923 в количестве 3·106 микробных тел на мл среды, на стекле создают равномерный слой агара толщиной 1-2 мм, в застывшем агаре вырезают лунки диаметром 2 мм, в которые вносят растворы стандарта в концентрациях 0,5; 1,5; 3; 4; 5; 6 мкг/мл и испытуемые образцы.A method for determining the concentration of benzylpenicillin preparations in biological fluids by using the microbiological method of diffusion into agar, which includes preparing a test microbe preparation, creating an agar layer, cutting holes in the frozen agar, introducing standard solutions into them and pre-diluting the test samples, characterized in that as the test microbe is used Staphylococcus aureus ATCC 25923 in the amount of 3 · 106 microbial bodies per ml of medium, on the glass create a uniform layer of agar 1-2 mm thick, in the frozen agar a notch holes 2 mm in diameter are introduced into which standard solutions are added in concentrations of 0.5; 1.5; 3; four; 5; 6 μg / ml and test samples.

Claims (1)

Способ определения концентрации препаратов бензилпенициллина в биологических жидкостях путем использования микробиологического метода диффузии в агар, включающий приготовление препарата тест-микроба, создание слоя агара, вырезание в застывшем агаре лунок, введение в них растворов стандарта и предварительное разведение испытуемых образцов, отличающийся тем, что в качестве тест-микроба используют Staphylococcus aureus ATCC 25923 в количестве 3·106 микробных тел на мл среды, на стекле создают равномерный слой агара толщиной 1-2 мм, в застывшем агаре вырезают лунки диаметром 2 мм, в которые вносят растворы стандарта в концентрациях 0,5; 1,5; 3; 4; 5; 6 мкг/мл и испытуемые образцы. A method for determining the concentration of benzylpenicillin preparations in biological fluids by using the microbiological method of diffusion into agar, including preparing a test microbe preparation, creating an agar layer, cutting holes in the frozen agar, introducing standard solutions into them and pre-diluting the test samples, characterized in that as test microbe used Staphylococcus aureus ATCC 25923 in an amount of 3 × 10 6 microbial cells per ml of medium, on glass create a uniform layer of agar 1-2 mm thick frozen in agar expr zayut wells 2 mm in diameter, in which make standard solutions at concentrations of 0.5; 1.5; 3; four; 5; 6 μg / ml and test samples.
RU2007146813/15A 2007-12-17 2007-12-17 Method of biological fluid analysis for concentration of benzylpenicillin preparations RU2379686C2 (en)

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RU2007146813/15A RU2379686C2 (en) 2007-12-17 2007-12-17 Method of biological fluid analysis for concentration of benzylpenicillin preparations

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RU2007146813/15A RU2379686C2 (en) 2007-12-17 2007-12-17 Method of biological fluid analysis for concentration of benzylpenicillin preparations

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RU2379686C2 RU2379686C2 (en) 2010-01-20

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RU2751795C1 (en) * 2020-07-21 2021-07-16 Общество с ограниченной ответственностью "Экотол-Сервис" Method for determining the concentration of didecyldimethylammonium chloride and polyhexamethylenebiguanidine hydrochloride in fecal waste from vehicles

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Effective date: 20091218