RU2006144369A - METHOD FOR EVALUATING CLINICAL SIGNIFICANCE OF CLOSTRIDIA - Google Patents
METHOD FOR EVALUATING CLINICAL SIGNIFICANCE OF CLOSTRIDIA Download PDFInfo
- Publication number
- RU2006144369A RU2006144369A RU2006144369/15A RU2006144369A RU2006144369A RU 2006144369 A RU2006144369 A RU 2006144369A RU 2006144369/15 A RU2006144369/15 A RU 2006144369/15A RU 2006144369 A RU2006144369 A RU 2006144369A RU 2006144369 A RU2006144369 A RU 2006144369A
- Authority
- RU
- Russia
- Prior art keywords
- monolayer
- clostridia
- clinical significance
- standard amount
- medium
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Способ оценки клинической значимости клостридий, отличающийся тем, что в условиях анаэростата в течение 2 ч стандартное количество суточной культуры микроорганизмов (1·10КОЕ/мл) в стандартном количестве накопительной среды СКС 0,2 мл инкубируют совместно с монослоем фибробластов кожи эмбриона человека и после трехкратного отмывания клеток монослоя средой Игла методом микроскопии оценивают наличие и силу действия токсина по степени разрушения монослоя в соответствии со шкалой Фармакопеи США.A method for assessing the clinical significance of clostridia, characterized in that under anaerostat conditions for 2 hours a standard amount of daily culture of microorganisms (1 · 10 CFU / ml) in a standard amount of SCS storage medium 0.2 ml is incubated together with a monolayer of fibroblasts of the skin of a human embryo and after three washing the monolayer cells with Eagle’s medium, the presence and strength of the toxin are evaluated by microscopy according to the degree of destruction of the monolayer in accordance with the US Pharmacopoeia scale.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2006144369/15A RU2348037C2 (en) | 2006-12-12 | 2006-12-12 | Method for assessment of clinical significance of clostridia |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2006144369/15A RU2348037C2 (en) | 2006-12-12 | 2006-12-12 | Method for assessment of clinical significance of clostridia |
Publications (2)
Publication Number | Publication Date |
---|---|
RU2006144369A true RU2006144369A (en) | 2008-06-20 |
RU2348037C2 RU2348037C2 (en) | 2009-02-27 |
Family
ID=40530035
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2006144369/15A RU2348037C2 (en) | 2006-12-12 | 2006-12-12 | Method for assessment of clinical significance of clostridia |
Country Status (1)
Country | Link |
---|---|
RU (1) | RU2348037C2 (en) |
-
2006
- 2006-12-12 RU RU2006144369/15A patent/RU2348037C2/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
RU2348037C2 (en) | 2009-02-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EA200870046A1 (en) | NUCLEAR REPROGRAMMING FACTOR | |
ES2422739T3 (en) | Composition for the diagnosis, prevention or treatment of diseases related to cells expressing IL-8 or GRO-, comprising UCB MSC | |
GB2434157A (en) | Platelets from stem cells | |
RU2012105922A (en) | DIFFERENTIATION OF HUMAN EMBRYONAL STEM CELLS | |
AR055176A1 (en) | DUPLICATION OF CHROMOSOME IN HAPLOID EMBRYOS OF CORN | |
BR112014006244A2 (en) | microorganisms and methods for producing alkenes | |
CN103898058B (en) | A kind of three-dimensional culture method of novel gum knurl stem cell and its application | |
Nazempour et al. | Combined effects of oscillating hydrostatic pressure, perfusion and encapsulation in a novel bioreactor for enhancing extracellular matrix synthesis by bovine chondrocytes | |
ATE371019T1 (en) | ARTIFICIAL IMMUNE ORGAN | |
ATE465637T1 (en) | CYSTEINE GRANULES AND THEIR USE AS GROWTH STIMULANTS FOR BIFIDOBACETERIUM ANIMALIS LACTIS | |
FR2903419B1 (en) | NOVEL TRICHODERMA ATROVIRIDE STRAIN, A CULTURE MEDIUM CONTAINING IT, AND THE USE OF SAID STRAIN, IN PARTICULAR AS STIMULANT OF GERMINATION AND / OR GROWTH OF PLANTS | |
EA201101626A1 (en) | BACTERIA WITH HIGHLY EFFICIENT METABOLISM | |
AR046123A1 (en) | SIMULATION OF MOTHER SIMIL CELLS, USE OF THE SAME | |
MY148336A (en) | Collection and selection methods to isolate a stem cell population from human adult periodontal follicular tissues | |
EA200800722A1 (en) | MEANS ACTIVATING STEM CELLS AND / OR PRECAUTIONAL CELLS | |
RU2008149763A (en) | STRAIN OF BACTERIA CLOSTRIDIUM ACETOBUTYLICUM-PRODUCER OF n-BUTYL ALCOHOL, ACETONE AND ETHANOL | |
RU2006144369A (en) | METHOD FOR EVALUATING CLINICAL SIGNIFICANCE OF CLOSTRIDIA | |
WO2007113591A3 (en) | Three dimensional cell culture | |
Wenger et al. | Pneumatic pressure bioreactor for cyclic hydrostatic stress application: mechanobiology effects on periodontal ligament cells | |
RU2005110248A (en) | METHOD FOR ASSESSING SPECIFIC ACTIVITY OF BACTERIOPHAGES | |
MX336067B (en) | Stem cell for therapeutic use which is derived from human monocyte, and method for inducing same. | |
ES2660763T3 (en) | Genetically encoded probe for the quantification of pyruvate concentration and methods of use thereof | |
MX2007015127A (en) | Uses of oligonucleotides stimulatory of the mesenchymal stem cell proliferation. | |
CN102391985B (en) | Method for performing procedural oxygen concentration pretreatment before transplantation of mesenchymal stem cells | |
MD3128F1 (en) | Process for Spirulina platensis biomass obtaining |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20081213 |