RU2006133049A - METHOD FOR DETERMINING ANTAGONISTICALLY ACTIVE BACTERIA STRAINS - Google Patents

METHOD FOR DETERMINING ANTAGONISTICALLY ACTIVE BACTERIA STRAINS Download PDF

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Publication number
RU2006133049A
RU2006133049A RU2006133049/15A RU2006133049A RU2006133049A RU 2006133049 A RU2006133049 A RU 2006133049A RU 2006133049/15 A RU2006133049/15 A RU 2006133049/15A RU 2006133049 A RU2006133049 A RU 2006133049A RU 2006133049 A RU2006133049 A RU 2006133049A
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RU
Russia
Prior art keywords
cultures
determining
strain
test
bacteria strains
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RU2006133049/15A
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Russian (ru)
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RU2328734C1 (en
Inventor
Василий Николаевич Афонюшкин (RU)
Василий Николаевич Афонюшкин
Юрий Георгиевич Юшков (RU)
Юрий Георгиевич Юшков
Ольга Владимировна Шкред (RU)
Ольга Владимировна Шкред
Original Assignee
Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего ВостокаСибирского отделени Россельхозакадемии (ГНУ ИЭВСиДВ СО РАСХН) (RU)
Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего Востока Сибирского отделения Россельхозакадемии (ГНУ ИЭВСиДВ СО РАСХН)
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Application filed by Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего ВостокаСибирского отделени Россельхозакадемии (ГНУ ИЭВСиДВ СО РАСХН) (RU), Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего Востока Сибирского отделения Россельхозакадемии (ГНУ ИЭВСиДВ СО РАСХН) filed Critical Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего ВостокаСибирского отделени Россельхозакадемии (ГНУ ИЭВСиДВ СО РАСХН) (RU)
Priority to RU2006133049/15A priority Critical patent/RU2328734C1/en
Publication of RU2006133049A publication Critical patent/RU2006133049A/en
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Publication of RU2328734C1 publication Critical patent/RU2328734C1/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (3)

1. Способ определения антагонистически активных штаммов бактерий, включающий выделение чистых культур бактерий и изучение их роста в присутствии тест-штамма, отличающийся тем, что первичный посев производят на богатую питательную среду типа уриселект 4, после чего осуществляют пересев на мясопептонный агар культур, различающихся по морфологическим критериям и ферментативной активности, а в качестве тест-штамма используют Р.vulgaris.1. The method of determining antagonistically active strains of bacteria, including the selection of pure cultures of bacteria and the study of their growth in the presence of a test strain, characterized in that the primary sowing is performed on a rich nutrient medium such as uriselect 4, after which reseeding is carried out on meat-peptone agar cultures differing in morphological criteria and enzymatic activity, and P.vulgaris is used as a test strain. 2. Способ по п.1, отличающийся тем, что посев исследуемых культур и тест штамма производят одновременно.2. The method according to claim 1, characterized in that the sowing of the studied cultures and the strain test are performed simultaneously. 3. Способ по п.1, отличающийся тем, что культуры потенциальных пробиотических штаммов, которые не были подавлены ползучим ростом подвижного штамма Proteus vulgaris, считаются антагонистичными в отношении данного вида микроорганизма.3. The method according to claim 1, characterized in that cultures of potential probiotic strains that were not suppressed by the creeping growth of the motile strain of Proteus vulgaris are considered antagonistic to this type of microorganism.
RU2006133049/15A 2006-09-14 2006-09-14 Method of antagonist active bacteria strain evaluation RU2328734C1 (en)

Priority Applications (1)

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RU2006133049/15A RU2328734C1 (en) 2006-09-14 2006-09-14 Method of antagonist active bacteria strain evaluation

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Application Number Priority Date Filing Date Title
RU2006133049/15A RU2328734C1 (en) 2006-09-14 2006-09-14 Method of antagonist active bacteria strain evaluation

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RU2006133049A true RU2006133049A (en) 2008-03-20
RU2328734C1 RU2328734C1 (en) 2008-07-10

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2451085C2 (en) * 2010-06-02 2012-05-20 Государственное научное учреждение Якутский научно-исследовательский институт сельского хозяйства Россельхозакадемии Method for estimating antibacterial activity of aerobic sporous microorganisms bacillus subtilis
RU2704115C1 (en) * 2018-10-22 2019-10-24 Федеральное государственное бюджетное учреждение науки Сибирский федеральный научный центр агробиотехнологий Российской академии наук (СФНЦА РАН) Method of determining antagonist activity of bacterial strains with respect to pseudomonas aeruginosa
RU2711957C1 (en) * 2019-05-14 2020-01-23 Артем Викторович Лямин Method for primary sowing of clinical material for isolating non-tuberculosis micobacteria

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Effective date: 20190915