RU2006121712A - METHOD FOR DETERMINING ANTIBODIES IN BLOOD SERUM - Google Patents

METHOD FOR DETERMINING ANTIBODIES IN BLOOD SERUM Download PDF

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Publication number
RU2006121712A
RU2006121712A RU2006121712/15A RU2006121712A RU2006121712A RU 2006121712 A RU2006121712 A RU 2006121712A RU 2006121712/15 A RU2006121712/15 A RU 2006121712/15A RU 2006121712 A RU2006121712 A RU 2006121712A RU 2006121712 A RU2006121712 A RU 2006121712A
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RU
Russia
Prior art keywords
blood serum
antibodies
dna
electrode
determining antibodies
Prior art date
Application number
RU2006121712/15A
Other languages
Russian (ru)
Other versions
RU2320990C1 (en
Inventor
Герман Константинович Будников (RU)
Герман Константинович Будников
Геннадий Артурович Евтюгин (RU)
Геннадий Артурович Евтюгин
Анна Вениаминовна Порфирьева (RU)
Анна Вениаминовна Порфирьева
Лиди Ивановна Анчикова (RU)
Лидия Ивановна Анчикова
Елена Юрьевна Подшивалина (RU)
Елена Юрьевна Подшивалина
Гульнара Рифатовна Вагапова (RU)
Гульнара Рифатовна Вагапова
Original Assignee
ГОУ ВПО "Казанский государственный университет им. В.И. Уль нова-Ленина" (RU)
ГОУ ВПО "Казанский государственный университет им. В.И. Ульянова-Ленина"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by ГОУ ВПО "Казанский государственный университет им. В.И. Уль нова-Ленина" (RU), ГОУ ВПО "Казанский государственный университет им. В.И. Ульянова-Ленина" filed Critical ГОУ ВПО "Казанский государственный университет им. В.И. Уль нова-Ленина" (RU)
Priority to RU2006121712/15A priority Critical patent/RU2320990C1/en
Publication of RU2006121712A publication Critical patent/RU2006121712A/en
Application granted granted Critical
Publication of RU2320990C1 publication Critical patent/RU2320990C1/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (1)

Способ определения антител в сыворотке крови, включающий инкубирование сыворотки крови и ДНК с последующей регистрацией взаимодействия ДНК и антител, при которой диагностическим признаком присутствия антител в сыворотке крови являются кривые измерения параметров электрохимической ячейки, состоящей из измерительного электрода и электрода сравнения, отличающийся тем, что инкубирование сыворотки крови и ДНК проводят на измерительном электроде, который предварительно покрывают полианилином, в качестве электрода сравнения берут хлоридсеребряный электрод, измеряют э.д.с. во времени до и после контакта измерительного электрода с сывороткой крови, причем сыворотку крови предварительно разбавляют в объемном соотношении 1:(20-50).A method for determining antibodies in blood serum, including incubation of blood serum and DNA with subsequent registration of the interaction of DNA and antibodies, in which the diagnostic sign of the presence of antibodies in the blood serum is the measurement curves of the parameters of the electrochemical cell, consisting of a measuring electrode and a reference electrode, characterized in that the incubation blood serum and DNA are carried out on a measuring electrode, which is pre-coated with polyaniline, chlorine is taken as a reference electrode silver electrode, measured emf in time before and after the contact of the measuring electrode with blood serum, and blood serum is pre-diluted in a volume ratio of 1: (20-50).
RU2006121712/15A 2006-06-07 2006-06-07 Method for determining antibodies in blood serum RU2320990C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2006121712/15A RU2320990C1 (en) 2006-06-07 2006-06-07 Method for determining antibodies in blood serum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2006121712/15A RU2320990C1 (en) 2006-06-07 2006-06-07 Method for determining antibodies in blood serum

Publications (2)

Publication Number Publication Date
RU2006121712A true RU2006121712A (en) 2008-01-10
RU2320990C1 RU2320990C1 (en) 2008-03-27

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RU2006121712/15A RU2320990C1 (en) 2006-06-07 2006-06-07 Method for determining antibodies in blood serum

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RU (1) RU2320990C1 (en)

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Publication number Publication date
RU2320990C1 (en) 2008-03-27

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20090608

NF4A Reinstatement of patent

Effective date: 20101220

MM4A The patent is invalid due to non-payment of fees

Effective date: 20180608