RU2005136009A - METHOD FOR PRODUCING THE LACCASE ENZYME DRUG - Google Patents

METHOD FOR PRODUCING THE LACCASE ENZYME DRUG Download PDF

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Publication number
RU2005136009A
RU2005136009A RU2005136009/13A RU2005136009A RU2005136009A RU 2005136009 A RU2005136009 A RU 2005136009A RU 2005136009/13 A RU2005136009/13 A RU 2005136009/13A RU 2005136009 A RU2005136009 A RU 2005136009A RU 2005136009 A RU2005136009 A RU 2005136009A
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RU
Russia
Prior art keywords
range
producing
concentration
temperature
laccase enzyme
Prior art date
Application number
RU2005136009/13A
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Russian (ru)
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RU2349644C2 (en
Inventor
Елена Сергеевна Горшина (RU)
Елена Сергеевна Горшина
Валентин Васильевич Бирюков (RU)
Валентин Васильевич Бирюков
Тать на Витальевна Русинова (RU)
Татьяна Витальевна Русинова
Александр Иванович Ярополов (RU)
Александр Иванович Ярополов
Ольга Владимировна Морозова (RU)
Ольга Владимировна Морозова
Сергей Валерьевич Шлеев (RU)
Сергей Валерьевич Шлеев
Игорь Николаевич Щеблыкин (RU)
Игорь Николаевич Щеблыкин
Original Assignee
Государственное образовательное учреждение ОГРН 1037739174406"Московский государственный университет инженерной экологии" Федерального агентства по образованию (RU)
Государственное образовательное учреждение ОГРН 1037739174406"Московский государственный университет инженерной экологии" Федерального агентства по образованию
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Application filed by Государственное образовательное учреждение ОГРН 1037739174406"Московский государственный университет инженерной экологии" Федерального агентства по образованию (RU), Государственное образовательное учреждение ОГРН 1037739174406"Московский государственный университет инженерной экологии" Федерального агентства по образованию filed Critical Государственное образовательное учреждение ОГРН 1037739174406"Московский государственный университет инженерной экологии" Федерального агентства по образованию (RU)
Priority to RU2005136009/13A priority Critical patent/RU2349644C2/en
Publication of RU2005136009A publication Critical patent/RU2005136009A/en
Application granted granted Critical
Publication of RU2349644C2 publication Critical patent/RU2349644C2/en

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Claims (1)

Способ получения ферментного препарата лакказы, предусматривающий глубинное культивирование клеток базидиального гриба Trametes на среде, содержащей углеводы, аммонийные и фосфоросодержащие соли, источники микроэлементов, отличающийся тем, что параметры культивирования поддерживают в зависимости от концентрации в среде растворенного кислорода, в том числе при концентрации кислорода более 50% от насыщения температуру поддерживают в пределах 25-27°С, величину рН - в пределах 3,0-7,0; при концентрации кислорода в пределах 5-50% от насыщения - температуру в пределах 27-29°С, а величину рН в пределах 4,0-5,0, при концентрации растворенного кислорода 0-5% - температуру 30-33°С и рН 4,0-6,0.A method for producing an enzyme preparation of laccase, which involves the deep cultivation of Trametes basidiomycete cells on a medium containing carbohydrates, ammonium and phosphorus salts, trace elements, characterized in that the cultivation parameters are supported depending on the concentration of dissolved oxygen in the medium, including when the oxygen concentration is more 50% of saturation, the temperature is maintained in the range of 25-27 ° C, the pH is in the range of 3.0-7.0; when the oxygen concentration is in the range of 5-50% of saturation, the temperature is in the range of 27-29 ° C, and the pH is in the range of 4.0-5.0, when the concentration of dissolved oxygen is 0-5%, the temperature is 30-33 ° C and pH 4.0-6.0.
RU2005136009/13A 2005-11-21 2005-11-21 Method of obtaining laccase enzyme medicine RU2349644C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2005136009/13A RU2349644C2 (en) 2005-11-21 2005-11-21 Method of obtaining laccase enzyme medicine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2005136009/13A RU2349644C2 (en) 2005-11-21 2005-11-21 Method of obtaining laccase enzyme medicine

Publications (2)

Publication Number Publication Date
RU2005136009A true RU2005136009A (en) 2007-05-27
RU2349644C2 RU2349644C2 (en) 2009-03-20

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RU2005136009/13A RU2349644C2 (en) 2005-11-21 2005-11-21 Method of obtaining laccase enzyme medicine

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RU (1) RU2349644C2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2770690C1 (en) * 2021-06-23 2022-04-21 Федеральное государственное бюджетное образовательное учреждение высшего образования "Уфимский государственный нефтяной технический университет" Fomes fomentarius vkpm f-1531 strain - producer of phenol oxidase enzymes

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RU2349644C2 (en) 2009-03-20

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Effective date: 20111122